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Rabbit Recombinant Monoclonal FCGR2 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (AB228971), expandable thumbnail
  • Western blot - Anti-CD16 + CD32 antibody [EPR22345] (AB228971), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (AB228971), expandable thumbnail
  • Western blot - Anti-CD16 + CD32 antibody [EPR22345] (AB228971), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytIPWBIHC-P
Mouse
Not recommended
Not recommended
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.

Additional Targets

Fcgr3

Alternative names

CD32, Fcgr2b, Ly-17, Fcgr2, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, IgG Fc receptor II beta, Lymphocyte antigen 17, Fc-gamma RII, Fc-gamma-RIIB, FcRII

Recommended products

Rabbit Recombinant Monoclonal FCGR2 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22345
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD16/CD32 also known as FcγRIII and FcγRII are types of Fc gamma receptors. CD16 refers to FcγRIII while CD32 refers to FcγRII. These proteins are involved in binding the Fc region of IgG antibodies. CD16 has a mass of about 50-65 kDa whereas CD32 varies around 40-45 kDa depending on its isoform. These receptors are expressed on the surface of various immune cells like natural killer cells macrophages monocytes and some T cells playing important roles in immune responses.

Biological function summary

CD16/CD32 are critical for mediating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. These proteins do not generally form a part of a larger complex but interact directly with immunoglobulins. They enhance the ability of immune cells to clear pathogens and infected cells from the body. These receptors contribute to the activation of signaling pathways that promote the destruction of targets opsonized with antibodies thereby linking adaptive and innate immune responses.

Pathways

These receptors influence the immune signaling cascades such as the NF-kB and MAPK pathways. These pathways are important for inflammatory responses and cellular activation. CD16/CD32 interactions lead to pathways that involve downstream signaling molecules like Syk and PI3K. This allows for immune modulation and assists in communicating between different types of immune responses orchestrating a coordinated attack against pathogens.

Associated diseases and disorders

CD16/CD32 have been connected to autoimmune diseases and certain cancers. These receptors are implicated in disorders like rheumatoid arthritis where their function can become dysregulated contributing to chronic inflammation. In cancers CD16 is important for mediating immune cell interactions in therapies that use antibodies to target cancerous cells. CD32's expression also links to conditions like systemic lupus erythematosus where altered receptor function impacts disease progression often interacting with proteins involved in immune regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on Kupffer cells and sinusoidal cells of mouse liver (PMID:26512139) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971), expandable thumbnail

    Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

    Lane 1: His-tagged mouse CD16 recombinant protein (aa31-215), 20 ng

    Lane 2: His-tagged mouse CD32 recombinant protein (aa30-210), 20 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 21 kDa

    Exposure time: 6s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on stromal cells in mouse colon (PMID:26512139) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971), expandable thumbnail

    Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time:
    Lanes 1,2,3 and 5: 3 minutes;
    Lanes 4: 15 seconds.

    Bands between 30-55 KD are likely to be glycosylated forms of CD16 and CD32 (PMID: 9378972, PMID: 19503602).

    Negative control: bEnd.3 (PMID: 23911392)

    All lanes: Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

    Lane 1: Mouse spleen lysate at 20 µg

    Lane 2: Mouse thymus lysate at 20 µg

    Lane 3: Mouse liver lysate at 20 µg

    Lane 4: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

    Lane 5: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30-55 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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