AB228971

Anti-CD16 + CD32 antibody [EPR22345]

Name: Anti-CD16 + CD32 antibody [EPR22345] (ab228971) | Abcam
Brand: Abcam Limited
SKU: AB228971
Price: 210 USD
Availability: InStock
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal FCGR2 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 7 publications.

View Alternative Names

CD32, Fcgr2b, Ly-17, Fcgr2, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, IgG Fc receptor II beta, Lymphocyte antigen 17, Fc-gamma RII, Fc-gamma-RIIB, FcRII, CD16, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, Fcgr3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD16 with ab228971 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on stromal cells in mouse colon (PMID : 26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16 with ab228971 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on Kupffer cells and sinusoidal cells of mouse liver (PMID : 26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer pH 9.0).

Supplier Data

Western blot - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

Lane 1:

His-tagged mouse CD16 recombinant protein (aa31-215), 20 ng

Lane 2:

His-tagged mouse CD32 recombinant protein (aa30-210), 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 21 kDa

false

Exposure time: 6s

Lab

Western blot - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lanes 1,2,3 and 5 : 3 minutes; Lanes 4 : 15 seconds. Bands between 30-55 KD are likely to be glycosylated forms of CD16 and CD32 (PMID : 9378972, PMID : 19503602). Negative control : bEnd.3 (PMID : 23911392)

All lanes:

Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution

Lane 1:

Mouse spleen lysate at 20 µg

Lane 2:

Mouse thymus lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

Lane 5:

bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30-55 kDa

false

Carrier free

Anti-CD16 + CD32 antibody [EPR22345] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22345

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD16/CD32 also known as FcγRIII and FcγRII are types of Fc gamma receptors. CD16 refers to FcγRIII while CD32 refers to FcγRII. These proteins are involved in binding the Fc region of IgG antibodies. CD16 has a mass of about 50-65 kDa whereas CD32 varies around 40-45 kDa depending on its isoform. These receptors are expressed on the surface of various immune cells like natural killer cells macrophages monocytes and some T cells playing important roles in immune responses.

Biological function summary

CD16/CD32 are critical for mediating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. These proteins do not generally form a part of a larger complex but interact directly with immunoglobulins. They enhance the ability of immune cells to clear pathogens and infected cells from the body. These receptors contribute to the activation of signaling pathways that promote the destruction of targets opsonized with antibodies thereby linking adaptive and innate immune responses.

Pathways

These receptors influence the immune signaling cascades such as the NF-kB and MAPK pathways. These pathways are important for inflammatory responses and cellular activation. CD16/CD32 interactions lead to pathways that involve downstream signaling molecules like Syk and PI3K. This allows for immune modulation and assists in communicating between different types of immune responses orchestrating a coordinated attack against pathogens.

CD16/CD32 have been connected to autoimmune diseases and certain cancers. These receptors are implicated in disorders like rheumatoid arthritis where their function can become dysregulated contributing to chronic inflammation. In cancers CD16 is important for mediating immune cell interactions in therapies that use antibodies to target cancerous cells. CD32's expression also links to conditions like systemic lupus erythematosus where altered receptor function impacts disease progression often interacting with proteins involved in immune regulation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.

See full target information Fcgr2

Additional targets

Fcgr3

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Research and practice in thrombosis and haemostasis 9:103016 PubMed40994889

2025

Optimization of a Western blot protocol for the detection of low levels of tissue factor in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Megan V Perkins,Ana T A Sachetto,Nigel Mackman

Inflammation 48:713-729 PubMed39117789

2024

Neuroprotective Effects of AER-271 in a tMCAO Mouse Model: Modulation of Autophagy, Apoptosis, and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglong Mo,Chengmin Yang,Xingwu Zheng,Hui Lv,Sanyin Mao,Ning Liu,Qin Yang,Bao Liao,Meiling Yang,Zhicheng Lu,Lina Tang,Xiaorui Huang,Chongdong Jian,Xuebin Li,Jingwei Shang

Inflammation 48:541-556 PubMed38951357

2024

Autophagy Regulation Attenuates Neuroinflammation and Cognitive Decline in an Alzheimer's Disease Mouse Model with Chronic Cerebral Hypoperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Yang,Chengmin Yang,Hui Lv,Xingwu Zheng,Sanyin Mao,Ning Liu,Shenglong Mo,Bao Liao,Meiling Yang,Zhicheng Lu,Lina Tang,Xiaorui Huang,Chongdong Jian,Jingwei Shang

Tissue engineering and regenerative medicine 21:437-453 PubMed38308742

2024

α-Gal Nanoparticles in CNS Trauma: II. Immunomodulation Following Spinal Cord Injury (SCI) Improves Functional Outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Bhavani Gopalakrishnan,Uri Galili,Megan Saenger,Noah J Burket,Wendy Koss,Manjari S Lokender,Kaitlyn M Wolfe,Samantha J Husak,Collin J Stark,Luis Solorio,Abigail Cox,August Dunbar,Riyi Shi,Jianming Li

Alzheimer's research & therapy 15:33 PubMed36797783

2023

Early activation of Toll-like receptor-3 reduces the pathological progression of Alzheimer's disease in APP/PS1 mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Shang Wang,Taiyang Zhu,Wanyan Ni,Chao Zhou,Hui Zhou,Li Lin,Yuting Hu,Xiaoyu Sun,Jingjing Han,Yan Zhou,Guoliang Jin,Jie Zu,Hongjuan Shi,Xingxing Yang,Zuohui Zhang,Fang Hua

Frontiers in medicine 8:796743 PubMed35141243

2022

Shionone Attenuates Sepsis-Induced Acute Kidney Injury by Regulating Macrophage Polarization the ECM1/STAT5 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Biao Zhang,Yi Xue,Jin Zhao,Huojun Jiang,Jiaoli Zhu,Hao Yin,Yizhen Qiu,Aihao Hu,Lingqi Xu,Yi Song,Xin Wang

Frontiers in immunology 11:1746 PubMed33013828

2020

hUCMSCs Mitigate LPS-Induced Trained Immunity in Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wei Feng,Cheng Wu,Feng-Yin Liang,Tuo Lin,Wan-Qi Li,Ying-Hua Jing,Pei Dai,Hui-Xian Yu,Yue Lan,Zhong Pei,Guang-Qing Xu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD16 + CD32 antibody [EPR22345]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Western blot - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Western blot - Anti-CD16 + CD32 antibody [EPR22345] (AB228971)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (7)

Product promise