Rabbit Recombinant Monoclonal FCGR2 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
ICC/IF | Flow Cyt | IP | WB | IHC-P | |
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Mouse | Not recommended | Not recommended | Not recommended | Tested | Tested |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
Fcgr3
CD32, Fcgr2b, Ly-17, Fcgr2, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, IgG Fc receptor II beta, Lymphocyte antigen 17, Fc-gamma RII, Fc-gamma-RIIB, FcRII
Rabbit Recombinant Monoclonal FCGR2 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
CD16/CD32 also known as FcγRIII and FcγRII are types of Fc gamma receptors. CD16 refers to FcγRIII while CD32 refers to FcγRII. These proteins are involved in binding the Fc region of IgG antibodies. CD16 has a mass of about 50-65 kDa whereas CD32 varies around 40-45 kDa depending on its isoform. These receptors are expressed on the surface of various immune cells like natural killer cells macrophages monocytes and some T cells playing important roles in immune responses.
CD16/CD32 are critical for mediating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis. These proteins do not generally form a part of a larger complex but interact directly with immunoglobulins. They enhance the ability of immune cells to clear pathogens and infected cells from the body. These receptors contribute to the activation of signaling pathways that promote the destruction of targets opsonized with antibodies thereby linking adaptive and innate immune responses.
These receptors influence the immune signaling cascades such as the NF-kB and MAPK pathways. These pathways are important for inflammatory responses and cellular activation. CD16/CD32 interactions lead to pathways that involve downstream signaling molecules like Syk and PI3K. This allows for immune modulation and assists in communicating between different types of immune responses orchestrating a coordinated attack against pathogens.
CD16/CD32 have been connected to autoimmune diseases and certain cancers. These receptors are implicated in disorders like rheumatoid arthritis where their function can become dysregulated contributing to chronic inflammation. In cancers CD16 is important for mediating immune cell interactions in therapies that use antibodies to target cancerous cells. CD32's expression also links to conditions like systemic lupus erythematosus where altered receptor function impacts disease progression often interacting with proteins involved in immune regulation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on Kupffer cells and sinusoidal cells of mouse liver (PMID:26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution
Lane 1: His-tagged mouse CD16 recombinant protein (aa31-215), 20 ng
Lane 2: His-tagged mouse CD32 recombinant protein (aa30-210), 20 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 21 kDa
Exposure time: 6s
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on stromal cells in mouse colon (PMID:26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1,2,3 and 5: 3 minutes;
Lanes 4: 15 seconds.
Bands between 30-55 KD are likely to be glycosylated forms of CD16 and CD32 (PMID: 9378972, PMID: 19503602).
Negative control: bEnd.3 (PMID: 23911392)
All lanes: Western blot - Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution
Lane 1: Mouse spleen lysate at 20 µg
Lane 2: Mouse thymus lysate at 20 µg
Lane 3: Mouse liver lysate at 20 µg
Lane 4: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
Lane 5: bEnd.3 (mouse brain endothelioma) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 30-55 kDa
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