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Rabbit Recombinant Monoclonal CD160 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (AB274374), expandable thumbnail
  • Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (AB274374), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

CD160 antigenReceptor on immune cells capable to deliver stimulatory or inhibitory signals that regulate cell activation and differentiation. Exists as a GPI-anchored and as a transmembrane form, each likely initiating distinct signaling pathways via phosphoinositol 3-kinase in activated NK cells and via LCK and CD247/CD3 zeta chain in activated T cells (PubMed:11978774, PubMed:17307798, PubMed:19109136). Receptor for both classical and non-classical MHC class I molecules (PubMed:12486241, PubMed:9973372). In the context of acute viral infection, recognizes HLA-C and triggers NK cell cytotoxic activity, likely playing a role in anti-viral innate immune response (PubMed:12486241). On CD8+ T cells, binds HLA-A2-B2M in complex with a viral peptide and provides a costimulatory signal to activated/memory T cells (PubMed:9973372). Upon persistent antigen stimulation, such as occurs during chronic viral infection, may progressively inhibit TCR signaling in memory CD8+ T cells, contributing to T cell exhaustion (PubMed:25255144). On endothelial cells, recognizes HLA-G and controls angiogenesis in immune privileged sites (PubMed:16809620). Receptor or ligand for TNF superfamily member TNFRSF14, participating in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. Upon ligation of TNFRSF14, provides stimulatory signal to NK cells enhancing IFNG production and anti-tumor immune response (By similarity). On activated CD4+ T cells, interacts with TNFRSF14 and down-regulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (PubMed:18193050). In the context of bacterial infection, acts as a ligand for TNFRSF14 on epithelial cells, triggering the production of antimicrobial proteins and pro-inflammatory cytokines (By similarity).CD160 antigen, soluble formThe soluble GPI-cleaved form, usually released by activated lymphocytes, might play an immune regulatory role by limiting lymphocyte effector functions.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD160 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23644-24

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD160 also known as BY55 is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular weight of approximately 27 kDa. This protein is expressed on the surface of natural killer (NK) cells CD8+ T cells and certain NKT cells. It functions as a receptor for MHC class I molecules and HLA-C playing a role in regulating immune responses. Researchers have also identified its expression in some endothelial cells.

Biological function summary

The protein CD160 plays a role in immune modulation by interacting with major histocompatibility complex (MHC) class I ligands. It participates in the immune response by enhancing cytotoxic activity in NK and CD8+ T cells therefore influencing cell-mediated immunity. CD160 does not associate with large signal transduction complexes but operates as a standalone receptor capable of mediating cellular effects upon ligand binding. Although primarily known for its role in immune modulation CD160 can impact endothelial functions suggesting a broader biological significance.

Pathways

CD160 engages in the pathway associated with immune cell signaling. It operates within the natural killer cell signaling cascade contributing to the regulation of cell-mediated cytotoxicity. In the context of adaptive immunity CD160 influences the T cell receptor signaling pathway indirectly coordinating its actions with proteins such as NKG2D and PD-1. These interactions are important in how immune cells assess threats and modulate responses.

Associated diseases and disorders

CD160 has implications in chronic inflammatory and autoimmune conditions. It is linked to disorders such as inflammatory bowel disease and psoriasis where immune regulation is disrupted. Its interaction with other proteins such as PD-1 may either exacerbate or alleviate symptoms depending on the pathological context. Understanding CD160's role provides insights into therapeutic strategies aimed at modulating immune responses in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374), expandable thumbnail

    Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD160 with ab274374 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab274374. Then stained with anti-CD8 alpha conjugated to Pacific blue.

    Gated on viable cells.

  • Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374), expandable thumbnail

    Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD160 with ab274374 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Monocytes (PMID: 30770415). Monocytes are the SSC high population. No positive signal was detected in this population.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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