AB234107

Anti-CD161 antibody [EPR21236]

Name: Anti-CD161 antibody [EPR21236] (ab234107) | Abcam
Brand: Abcam Limited
SKU: AB234107
Price: 345 USD
Availability: InStock
Rating: 4 (2 reviews)

BOND RX™ Validated
Recombinant
What is this?

(2 Reviews)

(9 Publications)

Rabbit Recombinant Monoclonal KLRBA antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 9 publications.

View Alternative Names

CD161a, Ly55, Ly55a, Nkrp1a, Klrb1a, Killer cell lectin-like receptor subfamily B member 1A, NKR-P1A, CD161 antigen-like family member A, Lymphocyte antigen 55A, NKR-P1.7, Natural killer cell surface protein P1-2, Ly-55A, NKR-P1 2

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR21236] (AB234107)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR21236] (AB234107)

IHC image of CD161 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica BOND^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab234107, 0.01μg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21236

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.01-0.025 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD161 also known as NKRP1A or KLRB1 is a type II transmembrane protein expressed primarily on natural killer (NK) cells and subsets of T cells. Weighing approximately 30-40 kDa CD161 functions as a C-type lectin receptor involved in the immune response. Its expression can be detected in various tissues including the liver and spleen. Researchers utilize tools such as mouse CD161 PE as well as CD161 APC antibodies for study of its cell surface expression and role in immune modulation.

Biological function summary

CD161 plays a role in immune system regulation by mediating NK cell and T cell activity. It does not operate as part of a complex but interacts with other cellular receptors to modulate immune responses. For example it serves as an inhibitory receptor often through engagement with its ligand LLT1 to temper immune responses and prevent overactivation. This balance is key to maintaining immune homeostasis and facilitates pathogen clearance while avoiding excessive tissue damage.

Pathways

CD161 is involved in the immune checkpoint pathways and is linked to inflammatory response signaling. These pathways play an important role in the immune system's ability to discern self from non-self determining the intensity and character of the body's defense mechanisms. Other proteins such as CTLA-4 and PD-1 also function within these pathways contributing to the regulation of immune cell activity and preventing autoimmunity.

CD161 is associated with inflammatory diseases and cancer. Its involvement in immune checkpoints implicates it in conditions such as autoimmune diseases where it may fail to properly regulate immune activity including multiple sclerosis. In cancer CD161 expression on T cells has been examined in the context of its inhibitory role potentially modulating the immune system's ability to target tumor cells effectively. In these scenarios its interaction with LLT1 and other immune checkpoint proteins influences the disease course and therapeutic outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a stimulatory role on natural killer (NK) cell cytotoxicity.

See full target information Klrb1a

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39656086

2024

Tissue-resident natural killer cells support survival in pancreatic cancer through promotion of cDC1-CD8 T activity.

Applications

Unspecified application

Species

Unspecified reactive species

Simei Go,Constantinos Demetriou,Giampiero Valenzano,Sophie Hughes,Simone Lanfredini,Helen Ferry,Edward Arbe-Barnes,Shivan Sivakumar,Rachel Bashford-Rogers,Mark R Middleton,Somnath Mukherjee,Jennifer Morton,Keaton Jones,Eric O Neill

Science translational medicine 16:eadk0642 PubMed39292804

2024

Intratumoral radiation dose heterogeneity augments antitumor immunity in mice and primes responses to checkpoint blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Justin C Jagodinsky,Jessica M Vera,Won Jong Jin,Amanda G Shea,Paul A Clark,Raghava N Sriramaneni,Thomas C Havighurst,Ishan Chakravarthy,Raad H Allawi,KyungMann Kim,Paul M Harari,Paul M Sondel,Michael A Newton,Marka R Crittenden,Michael J Gough,Jessica R Miller,Irene M Ong,Zachary S Morris

Nature communications 15:1496 PubMed38383468

2024

Exosomes define a local and systemic communication network in healthy pancreas and pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Adem,Nuno Bastos,Carolina F Ruivo,Sara Sousa-Alves,Carolina Dias,Patrícia F Vieira,Inês A Batista,Bruno Cavadas,Dieter Saur,José C Machado,Dawen Cai,Sonia A Melo

Frontiers in immunology 14:1250920 PubMed38077388

2023

Autophagy-mediated NKG2D internalization impairs NK cell function and exacerbates radiation pneumonitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqing Wang,Xinyue Ma,Xinyu Zhang,Dizhi Jiang,Hongyuan Mao,Zerun Li,Yu Tian,Bo Cheng

Cell reports. Medicine 4:101206 PubMed37769655

2023

Ablation of ERO1A induces lethal endoplasmic reticulum stress responses and immunogenic cell death to activate anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Lihui Liu,Sini Li,Yan Qu,Hua Bai,Xiangyu Pan,Jian Wang,Zhijie Wang,Jianchun Duan,Jia Zhong,Rui Wan,Kailun Fei,Jiachen Xu,Li Yuan,Chao Wang,Pei Xue,Xue Zhang,Zixiao Ma,Jie Wang

Frontiers in immunology 14:1133177 PubMed36993983

2023

Protective anti-tumor vaccination against glioblastoma expressing the MHC class II transactivator CIITA.

Applications

Unspecified application

Species

Unspecified reactive species

Fabrizio Celesti,Andrea Gatta,Mariam Shallak,Anna Maria Chiaravalli,Michele Cerati,Fausto Sessa,Roberto S Accolla,Greta Forlani

The Journal of cell biology 220: PubMed34287648

2021

The glycoprotein GP130 governs the surface presentation of the G protein-coupled receptor APLNR.

Applications

Unspecified application

Species

Unspecified reactive species

Kilian Trillet,Kathryn A Jacobs,Gwennan André-Grégoire,An Thys,Clément Maghe,Jonathan Cruard,Stéphane Minvielle,Sara Gonzalez Diest,Guillaume Montagnac,Nicolas Bidère,Julie Gavard

PloS one 15:e0226444 PubMed32240177

2020

Temporal and spatial modulation of the tumor and systemic immune response in the murine Gl261 glioma model.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly J McKelvey,Amanda L Hudson,Ramyashree Prasanna Kumar,James S Wilmott,Grace H Attrill,Georgina V Long,Richard A Scolyer,Stephen J Clarke,Helen R Wheeler,Connie I Diakos,Viive M Howell

Nature communications 11:1335 PubMed32165639

2020

Cancer immune control needs senescence induction by interferon-dependent cell cycle regulator pathways in tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Ellen Brenner,Barbara F Schörg,Fatima Ahmetlić,Thomas Wieder,Franz Joachim Hilke,Nadine Simon,Christopher Schroeder,German Demidov,Tanja Riedel,Birgit Fehrenbacher,Martin Schaller,Andrea Forschner,Thomas Eigentler,Heike Niessner,Tobias Sinnberg,Katharina S Böhm,Nadine Hömberg,Heidi Braumüller,Daniel Dauch,Stefan Zwirner,Lars Zender,Dominik Sonanini,Albert Geishauser,Jürgen Bauer,Martin Eichner,Katja J Jarick,Andreas Beilhack,Saskia Biskup,Dennis Döcker,Dirk Schadendorf,Leticia Quintanilla-Martinez,Bernd J Pichler,Manfred Kneilling,Ralph Mocikat,Martin Röcken

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