Anti-CD161 antibody [EPR26340-6] (BSA and Azide free)

• mIHC

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Multiplex immunohistochemistry - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded sections of human NK-T lymphoma tissue labeling CD-161 with ab302564 at 1 : 100 dilution (4.96 μg/mL) colored in red (Opal™570, red). Panel A : merged staining of anti-CD3 (ab16669, grey; Opal™690), anti-CD161(ab302564, red; Opal™570) and anti-NKG2A (ab260035, green; Opal™520) on human NK-T lymphoma. Panel B : anti-CD161 staining only. ab302564 (Opal™ 570, Red) at 1/100 dilution (4.96 µg/mL) Panel C : anti-NKG2A staining only. ; ab260035, (Opal™ 520, Green) NKG2A at 1/2000 dilution (0.283 µg/mL) Panel D : anti-CD3 staining only. CD3 staining only; ab16669 (Opal™ 690, Gray) at 1/2000 dilution (0.0675 µg/mL) The section was incubated in three rounds of staining : in the order of ab16669 for 30 mins, ab302564 for 30 mins and ab260035 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear stained with DAPI. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling CD161 with ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human skeletal muscle. The section was incubated with ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling CD161 with ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of human endometrium. The section was incubated with ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD161 with ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T ((human tissue labeling CD161 with ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T transfected with a Myc-His tagged CD161 construct, no staining on (B) HEK-293T transfected with empty plasmid. The section was incubated with ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD161 with ab302564 at 1/100 dilution (4.96 µg/mL) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on immune cells of human colon. The section was incubated with ab302564 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling CD161 with ab302564 at 1/50 (9.92 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membrane and cytoplasmic staining in subsets of human PBMC. is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• Flow Cyt

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Flow Cytometry - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD161 with ab302564 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : B lymphocytes (CD19+). Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565)

This data was developed using ab302564, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD161 with ab302564 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD4 conjugated to APC/Fire™ 750. Gated on viable cells.