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Rabbit Recombinant Monoclonal KLRB1 antibody. Carrier free. Suitable for ICC/IF, IHC-P, mIHC, Flow Cyt and reacts with Human samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (AB302565), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PmIHCFlow CytIP
Human
Tested
Not recommended
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

    Alternative names

    Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, HNKR-P1a, Natural killer cell surface protein P1A, NKR-P1A, KLRB1, NKRP1A, CLEC5B

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    Rabbit Recombinant Monoclonal KLRB1 antibody. Carrier free. Suitable for ICC/IF, IHC-P, mIHC, Flow Cyt and reacts with Human samples.

    Alternative names

    Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, HNKR-P1a, Natural killer cell surface protein P1A, NKR-P1A, KLRB1, NKRP1A, CLEC5B

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR26340-6

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    +4°C

    Notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Supplementary info

    Activity summary

    CD161 also known as NKRP1A or KLRB1 is a type II transmembrane protein expressed primarily on natural killer (NK) cells and subsets of T cells. Weighing approximately 30-40 kDa CD161 functions as a C-type lectin receptor involved in the immune response. Its expression can be detected in various tissues including the liver and spleen. Researchers utilize tools such as mouse CD161 PE as well as CD161 APC antibodies for study of its cell surface expression and role in immune modulation.

    Biological function summary

    CD161 plays a role in immune system regulation by mediating NK cell and T cell activity. It does not operate as part of a complex but interacts with other cellular receptors to modulate immune responses. For example it serves as an inhibitory receptor often through engagement with its ligand LLT1 to temper immune responses and prevent overactivation. This balance is key to maintaining immune homeostasis and facilitates pathogen clearance while avoiding excessive tissue damage.

    Pathways

    CD161 is involved in the immune checkpoint pathways and is linked to inflammatory response signaling. These pathways play an important role in the immune system's ability to discern self from non-self determining the intensity and character of the body's defense mechanisms. Other proteins such as CTLA-4 and PD-1 also function within these pathways contributing to the regulation of immune cell activity and preventing autoimmunity.

    Associated diseases and disorders

    CD161 is associated with inflammatory diseases and cancer. Its involvement in immune checkpoints implicates it in conditions such as autoimmune diseases where it may fail to properly regulate immune activity including multiple sclerosis. In cancer CD161 expression on T cells has been examined in the context of its inhibitory role potentially modulating the immune system's ability to target tumor cells effectively. In these scenarios its interaction with LLT1 and other immune checkpoint proteins influences the disease course and therapeutic outcomes.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    8 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/100 dilution (4.96 µg/mL) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit). Positive staining on immune cells of human colon. The section was incubated with Anti-CD161 antibody [EPR26340-6] ab302564 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (BOND™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

    • Flow Cytometry (Intracellular) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Flow Cytometry (Intracellular) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.
      Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: B lymphocytes (CD19+).Gated on viable cells.

    • Flow Cytometry (Intracellular) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Flow Cytometry (Intracellular) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.
      Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD4 conjugated to APC/Fire™ 750.Gated on viable cells.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.
      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/50 (9.92 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membrane and cytoplasmic staining in subsets of human PBMC. is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human skeletal muscle. The section was incubated with Anti-CD161 antibody [EPR26340-6] ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of human endometrium. The section was incubated with Anti-CD161 antibody [EPR26340-6] ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded (A) HEK-293T ((human tissue labeling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T transfected with a Myc-His tagged CD161 construct, no staining on (B) HEK-293T transfected with empty plasmid. The section was incubated with Anti-CD161 antibody [EPR26340-6] ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD161 antibody [EPR26340-6] (BSA and Azide free) (ab302565)

      This data was developed using Anti-CD161 antibody [EPR26340-6] ab302564, the same antibody clone in a different buffer formulation.
      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD161 with Anti-CD161 antibody [EPR26340-6] ab302564 at 1/100 (4.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with Anti-CD161 antibody [EPR26340-6] ab302564 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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