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AB189915

Anti-CD163 antibody [EPR14643-36] - C-terminal

4

(3 Reviews)

|

(23 Publications)

Rabbit Recombinant Monoclonal CD163 antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 23 publications.

View Alternative Names

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)

Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed.Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)

Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)

Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.

All lanes:

Immunoprecipitation - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915)

Predicted band size: 125 kDa

false

Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)
  • WB

Supplier Data

Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (AB189915)

All lanes:

Western blot - Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915) at 1/5000 dilution

Lane 1:

Human placenta lysate at 20 µg

Lane 2:

Human fetal liver lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 125 kDa

false

  • Carrier free

    Anti-CD163 antibody [EPR14643-36] - Low endotoxin, Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD163 antibody [EPR14643-36] - C-terminal

  • Biotin

    Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14643-36

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/40", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval using EDTA based pH 9.0 buffer.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
See full target information CD163
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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5481 PubMed40593590

2025

Single-cell analysis reveals a longitudinal trajectory of meningioma evolution and heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Yoon Lee,Eun Jung Lee,Bo Yeon Seo,Jiwon Kim,Youjin Song,Dayoung Lee,Namsung Moon,Harim Koo,Chul-Kee Park,Min-Sung Kim,Serk In Park,Do-Hyun Nam,Doo-Sik Kong,Jason K Sa

Frontiers in oncology 15:1503517 PubMed39980556

2025

High and low dose radiotherapy combined with ICIs for MSS colorectal cancer patients with liver metastases: a phase I study (HaRyPOT).

Applications

Unspecified application

Species

Unspecified reactive species

Yuxuan Ding,Yong Feng,Yangfan Ye,Jiayi Shen,Chang Guo,Xia He,Liangjun Zhu,Lijun Wang

Theranostics 14:5123-5140 PubMed39267775

2024

EBV-associated epithelial cancers cells promote vasculogenic mimicry formation via a secretory cross-talk with the immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Xiang,Fengze Sun,Tingting Liu,Jingjing Zhao,Jieying Yang,Dijun Ouyang,Hao Chen,Qian Zhu,Qijing Wang,Yongqiang Li,Jia He,Chaopin Yang,Xinyi Yang,Yuanyuan Chen,Yan Tang,Desheng Weng,Qiuzhong Pan,Qi Yang,Jianchuan Xia

Frontiers in oncology 13:1226494 PubMed38023213

2023

Genomic profiling and immune landscape of olfactory neuroblastoma in China.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyun Yang,Zhiyi Wan,Enli Zhang,Yingshi Piao

World journal of surgical oncology 21:90 PubMed36899373

2023

Integrative analyses identify CD73 as a prognostic biomarker and immunotherapeutic target in intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Ye Sun,Zhang-Fu Yang,Zhu-Tao Wang,Gao Liu,Cheng Zhou,Jian Zhou,Jia Fan,Wei Gan,Yong Yi,Shuang-Jian Qiu

eLife 11: PubMed36201245

2022

Label-free imaging of M1 and M2 macrophage phenotypes in the human dermis in vivo using two-photon excited FLIM.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Kröger,Jörg Scheffel,Evgeny A Shirshin,Johannes Schleusener,Martina C Meinke,Jürgen Lademann,Marcus Maurer,Maxim E Darvin

Annals of clinical and translational neurology 9:1136-1146 PubMed35750465

2022

Phosphorylated alpha-synuclein in Iba1-positive macrophages in the skin of patients with Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hideki Oizumi,Kenshi Yamasaki,Hiroyoshi Suzuki,Saki Ohshiro,Yuko Saito,Shigeo Murayama,Yoko Sugimura,Takafumi Hasegawa,Kohji Fukunaga,Atsushi Takeda

EBioMedicine 78:103958 PubMed35316682

2022

Dynamic profiling of immune microenvironment during pancreatic cancer development suggests early intervention and combination strategy of immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Yang,Qi Zhang,Junli Wang,Yu Lou,Zhengtao Hong,Shumei Wei,Ke Sun,Jianing Wang,Yiwen Chen,Jianpeng Sheng,Wei Su,Xueli Bai,Tingbo Liang

Gut and liver 16:645-659 PubMed34933280

2021

CD40 Agonists Alter the Pancreatic Cancer Microenvironment by Shifting the Macrophage Phenotype toward M1 and Suppress Human Pancreatic Cancer in Organotypic Slice Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Chae Yoon Lim,Jae Hyuck Chang,Won Sun Lee,Jeana Kim,Il Young Park

Cell death & disease 12:1140 PubMed34880206

2021

Comprehensive transcriptomic characterization reveals core genes and module associated with immunological changes via 1619 samples of brain glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Wenping Ma,Wenhua Fan,Changyuan Ren,Jianbao Xu,Fan Zeng,Zhaoshi Bao,Tao Jiang,Zheng Zhao
View all publications
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Product promise

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