Anti-CD163 antibody [EPR19518] - BSA and Azide free

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Fluorescence multiplex immunohistochemical analysis of human liver (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-MASP2 stained on cytoplasm of hepatocytes (ab277520; gray; Opal™690) at 1 : 100 ( 5.22 μg/ml) [Panel B] , anti-CD163 stained on Kupffer cells (ab213612; green; Opal™520) at 1 : 8000 ( 0.13 μg/ml) [Panel C], and anti-eNOS tained on endothelial cells (ab252439; red; Opal™570) at 1 : 200 ( 3.005 μg/ml) [Panel D] on human liver. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab277520, ab213612, and ab252439 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining eNOS with ab317582 at a 1 : 1000 (1.004 ug/ml) dilution, ab317582 anti-eNOS used at 1 : 500 (1.032 ug/ml) dilution and ab213612 anti-CD163 used at a 1 : 8000 (0.13 ug/ml) dilution.

Panel A : merged staining of anti-GLUT2 (magenta; Opal™690), anti-eNOS (green; Opal™520) and anti-CD163 (gray; Opal™570) on human liver.
Panel B : anti-GLUT2 staining membrane of hepatocytes in human liver.
Panel C : anti-eNOS staining endothelium in human liver.
Panel D : anti-CD163 staining Kupffer cells in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab260003, ab317582 and ab213612 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Tissue Microarrays stained for "Anti-CD163 antibody [EPR19518]" using "ab182422"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab182422 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver labelling GLUT2 with ab260003 at 1/1000 (B), eNOS with ab317582 at 1/500 dilution (C) and CD163 with ab213612 at 1/8000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and Nuclear DNA was labeled with DAPI (shown in blue). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-GLUT2 (magenta; Opal™690), anti-eNOS (green; Opal™520) and anti-CD163 (gray; Opal™570) on human liver.
Panel B : anti-GLUT2 staining membrane of hepatocytes in human liver.
Panel C : anti-eNOS staining endothelium in human liver.
Panel D : anti-CD163 staining Kupffer cells in human liver.

The section was incubated in three rounds of staining : in the order of ab260003, ab317582 and ab213612 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Fluorescence multiplex immunohistochemical analysis of human liver (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-eNOS stained on endothelial cells (ab252439; red; Opal™570) at 1 : 1000 ( 1.004 μg/ml) [Panel B], anti-CD163 stained on Kupffer cells (ab213612; green; Opal™520) at 1 : 8000 ( 0.13 μg/ml) [Panel B], and anti-Glucose Transporter GLUT2 stained on membrane of hepatocytes (ab234440; gray; Opal™690) at 1 : 200 ( 3.005 μg/ml) [Panel D] on human liver. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab234440, ab213612, and ab252439 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD163 with ab182422 at 2 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab182422 anti CD163 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This data was developed using ab182422, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) treated with 50 ng/mL IL10 for 24 hours (Lower) / Untreated control (Upper) cells labelling CD163 with ab182422 at 1/100 compared with a Rabbit monoclonal IgG (ab172730) /Left isotype control and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 was used as the secondary antibody.

Gated on viable CD14 positive cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This data was developed using ab182422, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of SU-DHL-1 (human histiocytic lymphoblast-like cell) cells labelling CD163 with ab182422 at 1/100 compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 was used as the secondary antibody.

Gated on viable cells.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Immunohistochemical analysis of formalin fixed paraffin embedded Human tonsil labelling CD163 with ab213612 at a concentration of 0.5 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab213612 anti CD163 antibody EPR19518 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• mIHC

Collaborator

Multiplex immunohistochemistry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

10-color fluorescence multiplex immunohistochemical analysis of human lung cancer tissue (formalin-fixed paraffin-embedded section).

Merged staining of anti-FOXP3 (ab215206; Cyan; TG540N), anti-PD1 (ab52587; Red; TG700N), anti-CD163 (ab182422; Brown; TG650N), anti-HLA-DR (ab92511; Yellow; TG570N), anti-CD4 (ab133616; Violet; TG620N), anti-CD8 alpha (ab101500; Purple; TG540S), anti-CD20 (ab9475; Grey; TG660S), anti-CD68 (ab192847; Green; TG520N), anti-Cytokeratin 19 (ab52625; Light blue; TG440N). TG470SN (dark blue) was used as a nuclear counter stain. The inset image shows the separate CD68 signal.

The section was incubated in nine rounds of staining; in the order of ab215206 (1/100 dilution), ab52587 (1/200 dilution), ab182422 (1/300 dilution), ab92511 (1/200 dilution), ab133616 (1/600 dilution), ab101500 (1/300 dilution), ab9475 (1/100 dilution), ab192847 (1/300 dilution), ab52625 (1/400 dilution); each using a separate fluorescent tyramide signal amplification system.

Sodium citrate antigen retrieval (pH6.0) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

Image acquisition was performed with TissueFAXS Spectra (TissueGnostics).

This image is courtesy of TissueGnostics Asia Pacific Limited

• Flow Cyt

Lab

Flow Cytometry - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Flow cytometry analysis of human PBMC cells (Human peripheral blood mononuclear cell) labeling with ab182422 at 1/60 dilution, 11.23 μg/ml (red). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) was used as the secondary antibody at 1/2000.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This IHC data was generated using the same anti-CD163 antibody clone, EPR19518, in a different buffer formulation (cat# ab182422).

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on Kupffer cells of Human liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Hofbauer cells in human placenta is observed. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD163 with ab182422 at 2 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab182422 anti CD163 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD163 with ab182422 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed some cytoplasmic staining on mouse liver. The nuclear counterstain is DAPI (blue). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse spleen is observed. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Kupffer cells of mouse liver is observed. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling CD163 with ab182422 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Kupffer cells of rat liver is observed. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182422).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

Tissue Microarrays stained for "Anti-CD163 antibody [EPR19518]" using "ab182422"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab182422 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This IHC data was generated using the same anti-CD163 antibody clone, EPR19518, in a different buffer formulation (cat# ab182422).

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CD163 with ab182422 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

The result showed some cytoplasmic staining on mouse spleen.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

• WB

Lab

Western blot - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

This data was developed using ab182422, the same antibody clone in a different buffer formulation. Different batches of ab182422 were tested on Rat liver lysate at 2.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 150 kDa.

All lanes:

Western blot - Anti-CD163 antibody [EPR19518] (<a href='/en-us/products/primary-antibodies/cd163-antibody-epr19518-ab182422'>ab182422</a>)

Predicted band size: 125 kDa

false

• WB

Lab

Western blot - Anti-CD163 antibody [EPR19518] - BSA and Azide free (AB213612)

All lanes:

Western blot - Anti-CD163 antibody [EPR19518] - BSA and Azide free (ab213612)

Predicted band size: 125 kDa

false

Exposure time: 30s