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Rat Recombinant Monoclonal CD163 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (AB289979), expandable thumbnail
  • Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (AB289979), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (AB289979), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (AB289979), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (AB289979), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

3 products for Alternative Product

Target data

Function

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Alternative names

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

Recommended products

Rat Recombinant Monoclonal CD163 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19518
Purification technique
Ion exchange chromatography
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

This rat monoclonal chimeric antibody has been engineered from RabMab parent antibody (Anti-Iba1 antibody [EPR16588] ab178846). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using across absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.

Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

Associated diseases and disorders

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979), expandable thumbnail

    Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:9712057 & 16517975). Negative control: U937, THP-1 (PMID:16368951, 10648003). Exposure time: 37 seconds

    All lanes: Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979) at 1/1000 dilution

    Lane 1: SU-DHL-1 (human large cell lymphoma histiocytic), whole cell lysate at 20 µg

    Lane 2: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

    Lane 3: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (Goat Anti-Rat IgG Fc (HRP) preadsorbed ab97090) at 1/20000 dilution

    Observed band size: 150 kDa

    Exposure time: 37s

  • Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979), expandable thumbnail

    Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:9712057 & 16517975). Exposure time: Lane 1: 81 seconds
    Lane 2: 37 seconds

    All lanes: Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (Goat Anti-Rat IgG Fc (HRP) preadsorbed ab97090) at 1/20000 dilution

    Observed band size: 150 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in human tonsil. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kupffer cells in the human liver. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in the human placenta. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in mouse spleen. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (ab289979)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD163 with ab289979 at 1/1000 (1.066 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in rat spleen. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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