Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free (AB290004)

This data was developed using ab289979, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kupffer cells in the human liver. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free (AB290004)

This data was developed using ab289979, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in the human placenta. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free (AB290004)

This data was developed using ab289979, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in human tonsil. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free (AB290004)

This data was developed using ab289979, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD163 with ab289979 at 1/1000 (1.066 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in rat spleen. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free (AB290004)

This data was developed using ab289979, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD163 with ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in mouse spleen. The section was incubated with ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free (AB290004)

This data was developed using ab289979, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID : 9712057 & 16517975). Negative control : U937, THP-1 (PMID : 16368951, 10648003). Exposure time : 37 seconds

All lanes:

Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cd163-antibody-epr19518-rat-igg2a-chimeric-ab289979'>ab289979</a>) at 1/1000 dilution

Lane 1:

SU-DHL-1 (human large cell lymphoma histiocytic), whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

Lane 3:

U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-fc-hrp-preadsorbed-ab97090'>ab97090</a>) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) - BSA and Azide free (AB290004)

This data was developed using ab289979, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID : 9712057 & 16517975). Exposure time : Lane 1 : 81 seconds Lane 2 : 37 seconds

All lanes:

Western blot - Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) (<a href='/en-us/products/primary-antibodies/cd163-antibody-epr19518-rat-igg2a-chimeric-ab289979'>ab289979</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-fc-hrp-preadsorbed-ab97090'>ab97090</a>) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 37s