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Rat Recombinant Monoclonal CD163 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (AB290004), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (AB290004), expandable thumbnail
  • Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (AB290004), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (AB290004), expandable thumbnail
  • Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (AB290004), expandable thumbnail

Key facts

Isotype

IgG2a

Host species

Rat

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

3 products for Alternative Product

Target data

Function

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Alternative names

Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, M130, CD163

Recommended products

Rat Recombinant Monoclonal CD163 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Alternative names

Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, M130, CD163

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR19518

Purification technique

Ion exchange chromatography

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab290004 is a carrier free version of Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979.

This rat monoclonal chimeric antibody has been engineered from RabMab parent antibody (Anti-Iba1 antibody [EPR16588] ab178846). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using across absorbed Fc-reactive secondary antibodies are recommended.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.

Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

Associated diseases and disorders

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004)

    This data was developed using Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD163 with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 at 1/1000 (1.066 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in rat spleen. The section was incubated with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004)

    This data was developed using Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD163 with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in mouse spleen. The section was incubated with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004), expandable thumbnail

    Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004)

    This data was developed using Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:9712057 & 16517975). Negative control: U937, THP-1 (PMID:16368951, 10648003). Exposure time: 37 seconds

    All lanes: Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) (Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979) at 1/1000 dilution

    Lane 1: SU-DHL-1 (human large cell lymphoma histiocytic), whole cell lysate at 20 µg

    Lane 2: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

    Lane 3: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (Goat Anti-Rat IgG Fc (HRP) preadsorbed ab97090) at 1/20000 dilution

    Observed band size: 150 kDa

    Exposure time: 37s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:9712057 & 16517975). Negative control: U937, THP-1 (PMID:16368951, 10648003). Exposure time: 37 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004)

    This data was developed using Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD163 with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in the human placenta. The section was incubated with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004), expandable thumbnail

    Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004)

    This data was developed using Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:9712057 & 16517975). Exposure time: Lane 1: 81 seconds
    Lane 2: 37 seconds

    All lanes: Western blot - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) (Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rat IgG Fc (HRP) preadsorbed (Goat Anti-Rat IgG Fc (HRP) preadsorbed ab97090) at 1/20000 dilution

    Observed band size: 150 kDa

    Exposure time: 37s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:9712057 & 16517975). Exposure time: Lane 1: 81 seconds
    Lane 2: 37 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004)

    This data was developed using Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD163 with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kupffer cells in the human liver. The section was incubated with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [EPR19518] – Rat IgG2a (Chimeric) – BSA and Azide free (ab290004)

    This data was developed using Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD163 with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 at 1/250 (4.264 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages in human tonsil. The section was incubated with Anti-CD163 antibody [EPR19518] - Rat IgG2a (Chimeric) ab289979 for 30 mins at room temperature, followed by anti-Rat IgG antibody (Rabbit Anti-Rat IgG H&L preadsorbed ab102248) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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