Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)

Rabbit Recombinant Multiclonal CD163 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-CD163 antibody [RM1114] - BSA and Azide free (AB316219), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt (Intra)	ICC/IF	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Tested	Expected	Not recommended
Rat	Expected	Expected	Tested	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information CD163

Function

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Alternative names

CD163, M130, CD163, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

Recommended products

Rabbit Recombinant Multiclonal CD163 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1114
Purification technique: Affinity purification Protein A
Specificity: Not suitable for mouse and rat WB.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab316219 is the carrirer-free version of Anti-CD163 antibody [RM1114] ab316218.

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary: CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
Biological function summary: CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.
Pathways: CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.
Associated diseases and disorders: CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Flow Cytometry (Intracellular) - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte, Left) / SU-DHL-1 (human histiocytic lymphoblast-like cell, Right) cells labelling CD163 with Anti-CD163 antibody [RM1114] ab316218 at 1/500 dilution (0.1 ug)/(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: THP-1.
Immunocytochemistry/ Immunofluorescence - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SU-DHL-1 (human histiocytic lymphoblast-like cell) cells labelling CD163 with Anti-CD163 antibody [RM1114] ab316218 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic staining in SU-DHL-1 cell line.

Negative control: THP-1 (PMID: 10648003).

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Left Panel is applied with Anti-CD163 antibody [RM1114] (Anti-CD163 antibody [RM1114] ab316218) at 1/50 dilution;

Right panel is applied with Anti-CD163 antibody (Anti-CD163 antibody ab87099) at 1/50 dilution.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Western blot - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Negative control: U-937 and THP-1(PMID:10648003).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 180 seconds; Lane 3-6: 26 seconds
All lanes: Western blot - Anti-CD163 antibody [RM1114] (Anti-CD163 antibody [RM1114] ab316218) at 1/1000 dilution
Lane 1: Human spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human placenta tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 5: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 6: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 150 kDa, 36 kDa
Western blot - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Left Panel is applied with Anti-CD163 antibody [RM1114] (Anti-CD163 antibody [RM1114] ab316218) at 1/1000 dilution (0.494 ug/ml); Right panel is applied with Anti-CD163 antibody (Anti-CD163 antibody ab87099) at 1/1000 dilution (0.2 ug/ml).
Exposure time: Lanes 1-2: 180 seconds; Lane 3-4: 26 seconds
All lanes: Western blot - Anti-CD163 antibody [RM1114] (Anti-CD163 antibody [RM1114] ab316218) at 1/1000 dilution
Lane 1: Human spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human placenta tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 150 kDa
Immunoprecipitation - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
CD163 was immunoprecipitated from 0.35 mg SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate with Anti-CD163 antibody [RM1114] ab316218 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD163 antibody [RM1114] ab316218 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate

Lane 2: Anti-CD163 antibody [RM1114] ab316218 IP in SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD163 antibody [RM1114] ab316218 in SU-DHL-1 whole cell lysate
All lanes: Immunoprecipitation - Anti-CD163 antibody [RM1114] (Anti-CD163 antibody [RM1114] ab316218) at 1/30 dilution
All lanes: SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366)
Exposure time: 15s
CD163 was immunoprecipitated from 0.35 mg SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate with Anti-CD163 antibody [RM1114] ab316218 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD163 antibody [RM1114] ab316218 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate

Lane 2: Anti-CD163 antibody [RM1114] ab316218 IP in SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD163 antibody [RM1114] ab316218 in SU-DHL-1 whole cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD163 with Anti-CD163 antibody [RM1114] ab316218 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen. The section was incubated with Anti-CD163 antibody [RM1114] ab316218 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD163 with Anti-CD163 antibody [RM1114] ab316218 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on kupffer cells of mouse liver. The section was incubated with Anti-CD163 antibody [RM1114] ab316218 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD163 with Anti-CD163 antibody [RM1114] ab316218 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with Anti-CD163 antibody [RM1114] ab316218 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD163 with Anti-CD163 antibody [RM1114] ab316218 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human spleen in (A) Anti-CD163 antibody [RM1114] ab316218 and (B) Anti-CD163 antibody ab87099.

The image on (A) Anti-CD163 antibody [RM1114] ab316218 is applied with Anti-CD163 antibody at 1/500 dilution and (B) Anti-CD163 antibody ab87099 is applied with Anti-CD163 antibody at 1/200 dilution.

The section was incubated with Anti-CD163 antibody [RM1114] ab316218 or Anti-CD163 antibody ab87099 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [RM1114] - BSA and Azide free (ab316219)
This data was developed using Anti-CD163 antibody [RM1114] ab316218, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CD163 with Anti-CD163 antibody [RM1114] ab316218 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on immune cells of human colon in (A) Anti-CD163 antibody [RM1114] ab316218, non-specific staining on human colon in (B) Anti-CD163 antibody ab87099.

The image on (A) Anti-CD163 antibody [RM1114] ab316218 is applied with Anti-CD163 antibody at 1/500 dilution and (B) Anti-CD163 antibody ab87099 is applied with Anti-CD163 antibody at 1/200 dilution.

The section was incubated with Anti-CD163 antibody [RM1114] ab316218 or Anti-CD163 antibody ab87099 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.