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AB17051

Anti-CD163 antibody [RM3/1]

1

(1 Review)

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(17 Publications)

Mouse Monoclonal CD163 antibody. Suitable for IHC-Fr, Flow Cyt (Intra) and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Cell preparation containing CD163 protein.

View Alternative Names

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

2 Images
Immunohistochemistry (Frozen sections) - Anti-CD163 antibody [RM3/1] (AB17051)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD163 antibody [RM3/1] (AB17051)

Immunohistochemistry analysis of frozen human tonsil tissue sections (interfollicular) stained for CD163 using ab17051 at a 1/10 dilution.

Flow Cytometry (Intracellular) - Anti-CD163 antibody [RM3/1] (AB17051)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD163 antibody [RM3/1] (AB17051)

Flow cytometric detection of CD163 in THP-1 cells. The black line represents cells only, the red line the isotype control and the blue line ab17051 in a concentration of 2 μg/250000 cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RM3/1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-Fr

applications

Immunogen

Cell preparation containing CD163 protein. The exact immunogen used to generate this antibody is proprietary information.

Q86VB7

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Reactivity data

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Product details

ab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment.
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Properties and storage information

Form
Liquid
Purification notes
0.2 µm filtered.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
See full target information CD163
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 52:815-831 PubMed32444799

2020

The FBW7-MCL-1 axis is key in M1 and M2 macrophage-related colon cancer cell progression: validating the immunotherapeutic value of targeting PI3Kγ.

Applications

Unspecified application

Species

Unspecified reactive species

Yeo Song Lee,Su Jeong Song,Hye Kyung Hong,Bo Young Oh,Woo Yong Lee,Yong Beom Cho

Nature immunology 21:615-625 PubMed32251403

2020

Blocking elevated p38 MAPK restores efferocytosis and inflammatory resolution in the elderly.

Applications

Unspecified application

Species

Unspecified reactive species

Roel P H De Maeyer,Rachel C van de Merwe,Rikah Louie,Olivia V Bracken,Oliver P Devine,Daniel R Goldstein,Mohib Uddin,Arne N Akbar,Derek W Gilroy

Experimental and therapeutic medicine 18:2503-2511 PubMed31572502

2019

The influence of PM on lung injury and cytokines in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yang,Yi Chen,Zhi Yu,Hui Ding,Zhongfu Ma

Cancer research 79:3891-3902 PubMed31186231

2019

SUMO-Specific Protease 1 Is Critical for Myeloid-Derived Suppressor Cell Development and Function.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Huang,Yong Zuo,Xiuzhi Wang,Xuefeng Wu,Hongsheng Tan,Qiuju Fan,Baijun Dong,Wei Xue,Guo-Qiang Chen,Jinke Cheng

Cancer cell international 18:95 PubMed29989015

2018

MiR-98 modulates macrophage polarization and suppresses the effects of tumor-associated macrophages on promoting invasion and epithelial-mesenchymal transition of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Li,Pengfei Sun,Chengsheng Zhang,Zongchao Li,Kai Cui,Wuyuan Zhou

Oncotarget 8:99801-99815 PubMed29245941

2017

Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer.

Applications

IHC-P, IHC-Fr

Species

Human, Human

Shuai Zhang,Dehai Che,Fang Yang,Chunling Chi,Hongxue Meng,Jing Shen,Li Qi,Fang Liu,Liyan Lv,Yue Li,Qingwei Meng,Junning Liu,Lihua Shang,Yan Yu

Cancer immunology, immunotherapy : CII 65:931-40 PubMed27271551

2016

Immunoglobulin G4 (IgG4)-positive plasma cell infiltration is associated with the clinicopathologic traits and prognosis of pancreatic cancer after curative resection.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaofei Liu,Zheyu Niu,Yuan Li,Mengyi Wang,Boju Pan,Zhaohui Lu,Quan Liao,Yupei Zhao

The Journal of rheumatology 42:979-87 PubMed25877505

2015

Elevated Serum Levels of Soluble CD163 in Polymyositis and Dermatomyositis: Associated with Macrophage Infiltration in Muscle Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Lin Peng,Yin-Li Zhang,Xiao-Ming Shu,Han-Bo Yang,Lu Zhang,Fang Chen,Xin Lu,Guo-Chun Wang

Connective tissue research 55:177-86 PubMed24649870

2014

Interleukin-1 receptor antagonist modulates inflammation and scarring after ligament injury.

Applications

Unspecified application

Species

Unspecified reactive species

Connie S Chamberlain,Ellen M Leiferman,Kayt E Frisch,Sarah E Duenwald-Kuehl,Stacey L Brickson,William L Murphy,Geoffrey S Baer,Ray Vanderby

PloS one 8:e79769 PubMed24324582

2013

High MUC2 expression in ovarian cancer is inversely associated with the M1/M2 ratio of tumor-associated macrophages and patient survival time.

Applications

IHC-P

Species

Human

Yi-feng He,Mei-ying Zhang,Xin Wu,Xiang-jun Sun,Ting Xu,Qi-zhi He,Wen Di
View all publications
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