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AB126756

Anti-CD163L1 antibody [EPR6539]

3

(1 Review)

|

(9 Publications)

Rabbit Recombinant Monoclonal CD163L1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications.

View Alternative Names

CD163b, CD163B, M160, UNQ6434/PRO23202, CD163L1, Scavenger receptor cysteine-rich type 1 protein M160, CD163 antigen-like 1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Unpurified ab126756 showing positive staining in Normal liver macrophages tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Unpurified ab126756 showing positive staining in Normal stomach macrophages tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Unpurified ab126756 showing positive staining in Normal tonsil macrophages tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Unpurified ab126756 at 1/100 dilution staining CD163L1 in paraffin-embedded Human spleen tissue by Immunohistochemsitry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Unpurified ab126756 showing negative staining in Normal placenta tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Immunohistochemical staining of paraffin embedded human stomach with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Unpurified ab126756 showing positive staining in Normal lung macrophages tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Unpurified ab126756 at 1/100 dilution staining CD163L1 in paraffin-embedded Human uterus tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163L1 antibody [EPR6539] (AB126756)

Immunohistochemical staining of paraffin embedded human spleen with purified ab126756 at a working dilution of 1/2000. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Carrier free

    Anti-CD163L1 antibody [EPR6539] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6539

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p><strong>For unpurified, use 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD163L1 also known as CD163-like 1 or M160 is a protein that acts as a scavenger receptor. This protein mechanically functions by binding to specific ligands and mediates endocytosis. CD163L1 belongs to the scavenger receptor cysteine-rich (SRCR) family which is involved in the clearance of hemoglobin-haptoglobin complexes. It is a type I membrane protein with an approximate mass of 150 kDa. The expression of CD163L1 is mainly detected in monocytes macrophages and certain dendritic cells usually in tissues associated with the immune response.
Biological function summary

This protein influences immune system processes related to inflammation. CD163L1 is part of the innate immune system and may be involved in the response to bacterial infections. It participates in the recognition and removal of apoptotic cells and pathogens. Though not a part of a protein complex its function aligns with receptors that mediate anti-inflammatory responses and tissue homeostasis in the body.

Pathways

This scavenger receptor associates with the anti-inflammatory pathway particularly in modulating responses to inflammation. It operates within a network involving the clearance and recycling of hemoglobin-heme. CD163L1 is linked with other proteins like CD163 which share similar clearing functions and SRCR domains are evident in their structure confirming their roles in host defense mechanisms.

CD163L1 shows relevance in conditions related to inflammation and immune dysfunction. For instance the receptor's activity is noted in the context of atherosclerosis as it can contribute to plaque stability due to its role in clearing hemoglobin-derived oxidants. It also connects to autoimmune diseases through its involvement with the dampening of inflammatory responses. In these conditions CD163L1 coordinates with proteins such as CD163 and other scavenger receptors that modulate immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CD163L1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

JOR spine 6:e1249 PubMed37361327

2023

Influence of macrophage polarization in herniated nucleus pulposus tissue on clinical efficacy after lumbar discectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Chuan Li,Shao-Jian Luo,Wu Fan,Cheng Jiang,Wei Wang,Jiong-Hui Chen,Yong-Long Chen,Zhen-Wu Zhang,Zhen-Hua Qiu,Dan-Qin Tan,Chun-Ming Huang,Mao-Sheng Wang,Xiao-Chun Bai

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 41:1335-1347 PubMed36370141

2022

Investigation of macrophage polarization in herniated nucleus pulposus of patients with lumbar intervertebral disc herniation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Chuan Li,Shao-Jian Luo,Fan Wu,Qing-Chun Mu,Jun-Hui Yang,Cheng Jiang,Wei Wang,Tian-Li Zhou,Tan-Dan Qin,Rong-Xiong Tan, Jian-Li,Chun-Ming Huang,Mao-Sheng Wang,Xiao-Chun Bai

International journal of molecular sciences 21: PubMed32545915

2020

A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep Dhall,Anne Lerch,Nicholas Johnson,Vimal Jacob,Brielle Jones,Min Sung Park,Malathi Sathyamoorthy

Cell reports 29:3488-3505.e9 PubMed31825831

2019

Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule.

Applications

Unspecified application

Species

Unspecified reactive species

Preethi Vijayaraj,Aspram Minasyan,Abdo Durra,Saravanan Karumbayaram,Mehrsa Mehrabi,Cody J Aros,Sarah D Ahadome,David W Shia,Katherine Chung,Jenna M Sandlin,Kelly F Darmawan,Kush V Bhatt,Chase C Manze,Manash K Paul,Dan C Wilkinson,Weihong Yan,Amander T Clark,Tammy M Rickabaugh,W Dean Wallace,Thomas G Graeber,Robert Damoiseaux,Brigitte N Gomperts

The spine journal : official journal of the North American Spine Society 18:343-356 PubMed29031872

2017

Accumulation and localization of macrophage phenotypes with human intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth R Nakazawa,Benjamin A Walter,Damien M Laudier,Divya Krishnamoorthy,Grace E Mosley,Kara L Spiller,James C Iatridis

Oncotarget 7:51733-51746 PubMed27351132

2016

Overexpression of semaphorin 3A promotes tumor progression and predicts poor prognosis in hepatocellular carcinoma after curative resection.

Applications

IHC-P

Species

Human

Zhi-Qiang Hu,Shao-Lai Zhou,Zheng-Jun Zhou,Chu-Bin Luo,Er-Bao Chen,Hao Zhan,Peng-Cheng Wang,Zhi Dai,Jian Zhou,Jia Fan,Xiao-Wu Huang

Cancer science 107:123-32 PubMed26663681

2016

Role of deltaNp63(pos)CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Shotaro Yamano,Min Gi,Yoshiyuki Tago,Kenichiro Doi,Satoshi Okada,Yukiyoshi Hirayama,Hirokazu Tachibana,Naomi Ishii,Masaki Fujioka,Kumiko Tatsumi,Hideki Wanibuchi

Oncogene 34:2471-82 PubMed24998851

2014

Loss of Cdh1 and Trp53 in the uterus induces chronic inflammation with modification of tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

G R Stodden,M E Lindberg,M L King,M Paquet,J A MacLean,J L Mann,F J DeMayo,J P Lydon,K Hayashi

Arthritis and rheumatism 65:1335-46 PubMed23335246

2013

Thymic stromal lymphopoietin is up-regulated in the skin of patients with systemic sclerosis and induces profibrotic genes and intracellular signaling that overlap with those induced by interleukin-13 and transforming growth factor β.

Applications

ICC/IF

Species

Human

Romy B Christmann,Allison Mathes,Alsya J Affandi,Cristina Padilla,Banafsheh Nazari,Andreea M Bujor,Giuseppina Stifano,Robert Lafyatis
View all publications
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