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AB231262

Anti-CD166 antibody

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(1 Publication)

Rabbit Polyclonal CD166 antibody. Suitable for WB and reacts with Mouse, Cow samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Cow ALCAM aa 1-550.

View Alternative Names

CD166, CD166 antigen, Activated leukocyte cell adhesion molecule, BEN, Protein DM-GRASP, Alcam

3 Images
Western blot - Anti-CD166 antibody (AB231262)
  • WB

Supplier Data

Western blot - Anti-CD166 antibody (AB231262)

All lanes:

Western blot - Anti-CD166 antibody (ab231262) at 2 µg/mL

All lanes:

Mouse cerebrum tissue lysate

Predicted band size: 65 kDa

false

Western blot - Anti-CD166 antibody (AB231262)
  • WB

Supplier Data

Western blot - Anti-CD166 antibody (AB231262)

All lanes:

Western blot - Anti-CD166 antibody (ab231262) at 2 µg/mL

All lanes:

Recombinant cow ALCAM protein.

Predicted band size: 65 kDa

false

Western blot - Anti-CD166 antibody (AB231262)
  • WB

Supplier Data

Western blot - Anti-CD166 antibody (AB231262)

All lanes:

Western blot - Anti-CD166 antibody (ab231262) at 2 µg/mL

All lanes:

Cow Cerebrum tissue lysate.

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Cow

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Cow ALCAM aa 1-550. The exact immunogen used to generate this antibody is proprietary information.

Q9BH13

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD166 also known as ALCAM (activated leukocyte cell adhesion molecule) is a cell surface glycoprotein with a mass of approximately 105 kDa. This protein is widely expressed on various cell types including activated T-cells keratinocytes neurons and endothelial cells. In the immune system CD166 has roles in T-cell activation and migration. It contains five extracellular immunoglobulin-like domains which facilitate cell-cell interactions. Researchers often regard ALCAM-Z as an alternative form sharing structural elements with CD166 protein.
Biological function summary

CD166 mediates cell-cell adhesion and plays roles in neuronal development. It participates in the formation of synaptic junctions and affects neuronal differentiation. CD166 is also a component of cell signaling complexes influencing cell proliferation and migration. In addition it controls interactions between T-cells and dendritic cells impacting immune response. Its interactions with other adhesion molecules contribute to cellular communication and tissue integrity.

Pathways

CD166 functions within signaling pathways related to cell adhesion and migration. It is involved in the Wnt signaling pathway and its interactions regulate cell polarity and morphogenesis. CD166 interacts closely with proteins such as CD6 and Catenins impacting cellular signaling and the organization of the cytoskeleton. These pathways affect processes like embryonic development and immune cell function.

CD166 has implications in cancer and autoimmune diseases. Its dysregulation links to cancer metastasis where altered expression facilitates tumor invasion and spread. CD166 also relates to autoimmune diseases through its role in T-cell activation potentially contributing to conditions like multiple sclerosis. The interaction between CD166 and CD6 influences immune cell activation and may offer therapeutic targets for modulating immune responses in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity). Mediates homotypic interactions with cells that express ALCAM (PubMed : 24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed : 23169771). Required for normal organization of the lymph vessel network (PubMed : 23169771). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed : 24740813). Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow (PubMed : 25730656). Promotes in vitro osteoblast proliferation and differentiation (PubMed : 25730656). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity). Mediates outgrowth and pathfinding for retinal ganglion cell axons (PubMed : 15345243).
See full target information Alcam
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 13: PubMed37238701

2023

Lipoxin A4 (LXA4) Reduces Alkali-Induced Corneal Inflammation and Neovascularization and Upregulates a Repair Transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Jiucheng He,Thang L Pham,Azucena H Kakazu,Abhilash Ponnath,Khanh V Do,Haydee E P Bazan
View all publications
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