Name: Anti-CD166 antibody [EPR2759(2)]
SKU: ab109215
Rating: 4 (3 reviews)

Anti-CD166 antibody [EPR2759(2)] (ab109215) is a rabbit monoclonal antibody that is used to detect CD166 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with CD166 knockout cell line validation

Related conjugates and formulations

ab219139
Conjugated
PE Anti-CD166 antibody [EPR2759(2)]
ab206127
Carrier free
Anti-CD166 antibody [EPR2759(2)] - BSA and Azide free
ab197543
Conjugated
Alexa Fluor® 488 Anti-CD166 antibody [EPR2759(2)]
ab196846
Conjugated
HRP Anti-CD166 antibody [EPR2759(2)]
ab313207
Conjugated
Alexa Fluor® 555 Anti-CD166 antibody [EPR2759(2)]
ab311726
Conjugated
Alexa Fluor® 594 Anti-CD166 antibody [EPR2759(2)]
ab310812
Conjugated
APC Anti-CD166 antibody [EPR2759(2)]
ab311107
Conjugated
Alexa Fluor® 647 Anti-CD166 antibody [EPR2759(2)]
ab313003
Conjugated
Alexa Fluor® 568 Anti-CD166 antibody [EPR2759(2)]
ab320934
Conjugated
Alexa Fluor® 750 Anti-CD166 antibody [EPR2759(2)]

Images

Flow Cytometry - Anti-CD166 antibody [EPR2759(2)] (AB109215), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	Flow Cyt	WB	ICC/IF
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Expected	Tested	Expected
Rat	Expected	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes For unpurified use at 1/10 - 1/100.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/90	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/20000	Notes For unpurified use at 1/1000 - 1/10000.
Species Human	Dilution info 1/10000 - 1/20000	Notes For unpurified use at 1/1000 - 1/10000.
Species Rat	Dilution info 1/10000 - 1/20000	Notes For unpurified use at 1/1000 - 1/10000.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information ALCAM

Function

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:15048703, PubMed:15496415, PubMed:16352806, PubMed:23169771, PubMed:24945728, PubMed:7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed:29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity). Isoform 3. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

Alternative names

CD166, MEMD, ALCAM, CD166 antigen, Activated leukocyte cell adhesion molecule

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR2759(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-CD166 antibody [EPR2759(2)] (ab109215) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CD166?

Anti-CD166 [EPR2759(2)] (ab109215) specifically detects a band for CD166 (UniProt: Q13740) at a molecular weight of 65kDa.

Trusted by the scientific community

Anti-CD166 [EPR2759(2)] (ab109215) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed

The specificity of Anti-CD166 antibody [EPR2759(2)] (ab109215) has been confirmed by Western blot testing in ALCAM Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EPR2759(2)] also available for your convenience:
ab109215, HRP - ab196846, Alexa Fluor® 488 - ab197543, Carrier free - ab206127, PE - ab219139, APC - ab310812, Alexa Fluor® 647 - ab311107, Alexa Fluor® 594 - ab311726, Alexa Fluor® 568 - ab313003, Alexa Fluor® 555 - ab313207, Alexa Fluor® 750 - ab320934

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD166 also known as ALCAM (activated leukocyte cell adhesion molecule) is a cell surface glycoprotein with a mass of approximately 105 kDa. This protein is widely expressed on various cell types including activated T-cells keratinocytes neurons and endothelial cells. In the immune system CD166 has roles in T-cell activation and migration. It contains five extracellular immunoglobulin-like domains which facilitate cell-cell interactions. Researchers often regard ALCAM-Z as an alternative form sharing structural elements with CD166 protein.

Biological function summary

CD166 mediates cell-cell adhesion and plays roles in neuronal development. It participates in the formation of synaptic junctions and affects neuronal differentiation. CD166 is also a component of cell signaling complexes influencing cell proliferation and migration. In addition it controls interactions between T-cells and dendritic cells impacting immune response. Its interactions with other adhesion molecules contribute to cellular communication and tissue integrity.

Pathways

CD166 functions within signaling pathways related to cell adhesion and migration. It is involved in the Wnt signaling pathway and its interactions regulate cell polarity and morphogenesis. CD166 interacts closely with proteins such as CD6 and Catenins impacting cellular signaling and the organization of the cytoskeleton. These pathways affect processes like embryonic development and immune cell function.

Associated diseases and disorders

CD166 has implications in cancer and autoimmune diseases. Its dysregulation links to cancer metastasis where altered expression facilitates tumor invasion and spread. CD166 also relates to autoimmune diseases through its role in T-cell activation potentially contributing to conditions like multiple sclerosis. The interaction between CD166 and CD6 influences immune cell activation and may offer therapeutic targets for modulating immune responses in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Flow Cytometry - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Flow Cytometry analysis of HuT-78 cells labelling CD166 with purified ab109215 at 1/90 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunoprecipitation - Anti-CD166 antibody [EPR2759(2)] (ab109215)
ab109215 (purified) at 1/30 immunoprecipitating CD166 in SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Predicted band size: 65 kDa
Observed band size: 100-105 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Immunocytochemistry/Immunofluorescence analysis of THP-1 (human monocytic leukemia cell line) cells labelling CD166 (green) with purified ab109215 at 1/250. Cells were fixed with 100% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CD166 with purified ab109215 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: CD166 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)
Lane 4: SH-SY5Y whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109215 observed at 100 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
ab109215 was shown to specifically react with CD166 in wild-type HAP1 cells as signal was lost in CD166 knockout cells. Wild-type and CD166 knockout samples were subjected to SDS-PAGE. ab109215 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Predicted band size: 65 kDa
Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215) at 1/10000 dilution
Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 20 µg
Lane 2: HuT-78 cell lysate at 20 µg
Lane 3: HT-1080 (human fibrosarcoma cell line) cell lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 65 kDa
Observed band size: 100-105 kDa
Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215) at 1/10000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 166 kDa, 52 kDa, 65 kDa
Observed band size: 100-105 kDa, 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling CD166 with unpurified ab109215 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215)
All lanes: Western blot - Anti-CD166 antibody [EPR2759(2)] (ab109215) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate at 10 µg
Lane 2: HuT-78 cell lysate at 10 µg
Lane 3: HT1080 (human fibrosarcoma cell line) cell lysate at 10 µg
Lane 4: Daudi (human Burkitt's lymphoma cell line) cell lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa
Observed band size: 100-105 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [EPR2759(2)] (ab109215)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic adenocarcinoma tissue labelling CD166 with unpurified ab109215 at 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.