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AB259544

Anti-CD167a/DDR1 antibody [EPR22316-2] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CD167a/DDR1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, DDR1, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK, MCK-10

1 Images
Sandwich ELISA - Anti-CD167a/DDR1 antibody [EPR22316-2] - BSA and Azide free (Detector) (AB259544)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CD167a/DDR1 antibody [EPR22316-2] - BSA and Azide free (Detector) (AB259544)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab260063).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22316-2

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab259544 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD167a/DDR1. The recommended pair for sandwich ELISA is:
Capture: ab259543, Human CD167a/DDR1 Capture Antibody (unconjugated)
Detector: ab259544, Human CD167a/DDR1 Detector Antibody (unconjugated)
The reference range value is 156.25 - 10000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD167a also known as DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase with a molecular mass of approximately 101 kDa. This protein is expressed widely in various tissues including the thyroid lung kidney and mammary gland. It becomes activated upon collagen binding leading to the regulation of cell adhesion proliferation and extracellular matrix remodeling. The DDR1 protein plays a mechanistic role in responding to unique collagen-rich environments within the body.
Biological function summary

CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.

Pathways

CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.

Mutations or irregular expression of CD167a/DDR1 have implications in cancer and fibrotic diseases. Overexpression or aberration of DDR1 can contribute to tumorigenesis facilitating tumor cell invasion and metastasis. In fibrotic conditions altered DDR1 activity can disrupt normal matrix remodeling linking it to proteins such as collagen I and III which play major roles in these disorders. Understanding DDR1's role offers potential for therapeutic interventions in targeting related pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
See full target information DDR1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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