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Knockout Tested Rabbit Monoclonal CD167a/DDR1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-CD167a/DDR1 antibody [EPR24783-7] (AB288675), expandable thumbnail
  • Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (AB288675), expandable thumbnail
  • Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (AB288675), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

Species
Mouse
Dilution info
10 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Tyrosine kinase that functions as a cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.

Alternative names

CD167a, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, DDR1, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2, Mammary carcinoma kinase 10, Protein-tyrosine kinase 3A, Protein-tyrosine kinase RTK-6, TRK E, Tyrosine kinase DDR, Tyrosine-protein kinase CAK, MCK-10

Recommended products

Knockout Tested Rabbit Monoclonal CD167a/DDR1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24783-7
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD167a also known as DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase with a molecular mass of approximately 101 kDa. This protein is expressed widely in various tissues including the thyroid lung kidney and mammary gland. It becomes activated upon collagen binding leading to the regulation of cell adhesion proliferation and extracellular matrix remodeling. The DDR1 protein plays a mechanistic role in responding to unique collagen-rich environments within the body.

Biological function summary

CD167a/DDR1 modulates interactions between cells and their surrounding matrix. It is involved in a complex with collagen mediating signal transduction processes. This interaction is important for cellular biophysical responses driving processes like cell migration and differentiation. In marmoset thyroid tissue DDR1 helps maintain structural integrity and regulates cellular activities important for normal thyroid function.

Pathways

CD167a/DDR1 participates in key signaling pathways including those related to cell adhesion and extracellular matrix remodeling. Specifically it operates within the MAPK/ERK pathway influencing cell cycle dynamics and responses to stress signals. DDR1 also interacts with proteins like SHC and Ras enabling communication between the cell surface and intracellular targets necessary for growth and adaptation.

Associated diseases and disorders

Mutations or irregular expression of CD167a/DDR1 have implications in cancer and fibrotic diseases. Overexpression or aberration of DDR1 can contribute to tumorigenesis facilitating tumor cell invasion and metastasis. In fibrotic conditions altered DDR1 activity can disrupt normal matrix remodeling linking it to proteins such as collagen I and III which play major roles in these disorders. Understanding DDR1's role offers potential for therapeutic interventions in targeting related pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675), expandable thumbnail

    Western blot - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675)

    Western blot: Anti-DDR1 antibody [EPR24783-7] (ab288675) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab288675 was shown to bind specifically to DDR1. A band was observed at 100-125 kDa in wild-type HeLa cell lysates with no signal observed at this size in DDR1 knockout cell line Human CD167a/DDR1 knockout MCF7 cell line ab280048 (knockout cell lysate Human CD167a/DDR1 knockout MCF7 cell lysate ab280107). To generate this image, wild-type and DDR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 20 µg

    Lane 2: NIH/3T3 cell lysate at 20 µg

    Lane 3: T-47D cell lysate at 20 µg

    Lane 4: Human Brain cell lysate at 20 µg

    Lane 5: Human Liver cell lysate at 20 µg

    Lane 6: Mouse Brain cell lysate at 20 µg

    Lane 7: Mouse Liver cell lysate at 20 µg

    Lane 8: Wild-type MCF7 Serum Starved (24 h) Vehicle Control Collagen I (0 ug/mL, 16 h) ab288242 cell lysate at 20 µg

    Lane 9: Wild-type MCF7 Serum Starved (24 h) Treated Collagen I (25 ug/mL, 16 h) ab288241 cell lysate at 20 µg

    Lane 10: DDR1 knockout MCF7 Serum Starved (24 h) Vehicle Control Collagen I (0 ug/mL, 16 h) ab288240 cell lysate at 20 µg

    Lane 11: DDR1 knockout MCF7 Serum Starved (24 h) Treated Collagen I (25 ug/mL, 16 h) ab288238 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 125 kDa

    Observed band size: 100 kDa, 125 kDa

  • Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675), expandable thumbnail

    Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675)

    Epithelial discoidin domain-containing receptor 1 was immunoprecipitated from 0.5 mg NIH/3T3 lysates with 5 μg ab288675. Western blot was performed on the immunoprecipitate using ab288675 at 1/1000.

    Lane 1: NIH/3T3 lysate at 10 µg

    Lane 2: NIH/3T3 Pulldown with 10 µg/mL ab288675

    Lane 3: NIH/3T3 Pulldown with 10 µg/mL Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 isotype control

    Observed band size: 120 kDa

    For Western blot, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % NFDM in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature in 3 % NFDM block, washed again four times then imaged.

    All lanes: Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675) at 10 µg/mL

    All lanes: NIH/3T3 lysate with 3 % NFDM in TBS-0.1% Tween® 20 (TBS-T)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Exposure time: 4min

  • Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675), expandable thumbnail

    Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675)

    Epithelial discoidin domain-containing receptor 1 was immunoprecipitated from 0.5 mg T-47D lysates with 5 μg ab288675. Western blot was performed on the immunoprecipitate using ab288675 at 1/1000.

    Lane 1: T-47D lysate at 10 µg

    Lane 2: T-47D Pulldown with 10 µg/mL ab288675

    Lane 3: T-47D Pulldown with 10 µg/mL Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 isotype control

    Observed band size: 120 kDa

    For Western blot, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % NFDM in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature in 3 % NFDM block, washed again four times then imaged.

    All lanes: Immunoprecipitation - Anti-CD167a/DDR1 antibody [EPR24783-7] (ab288675) at 10 µg/mL

    All lanes: T-47D lysate with 3 % NFDM in TBS-0.1% Tween® 20 (TBS-T)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Exposure time: 20min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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