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Rabbit Recombinant Monoclonal CD168 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications.


Images

Immunoprecipitation - Anti-CD168 antibody [EPR4054] (AB124729), expandable thumbnail
  • Western blot - Anti-CD168 antibody [EPR4054] (AB124729), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] (AB124729), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] (AB124729), expandable thumbnail
  • Western blot - Anti-CD168 antibody [EPR4054] (AB124729), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Expected
Not recommended
Tested
Expected
Rat
Not recommended
Expected
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

For unpurified use at 1/1000 - 1/10000.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

Species
Rat
Dilution info
1/1000
Notes

For unpurified use at 1/1000 - 1/10000.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/1000 - 1/10000.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

Tested
Tested

Species
Human
Dilution info
1/250
Notes

For unpurified use at 1/50 - 1/100.

Antigen retrieval is recommended

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

1 product for Alternative Version

Target data

Function

Receptor for hyaluronic acid (HA) (By similarity). Involved in cell motility (By similarity). When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. May act as a regulator of adipogenisis (By similarity).

Alternative names

CD168, IHABP, RHAMM, HMMR, Hyaluronan mediated motility receptor, Intracellular hyaluronic acid-binding protein, Receptor for hyaluronan-mediated motility

Recommended products

Rabbit Recombinant Monoclonal CD168 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 19 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4054
Purification technique
Affinity purification Protein A
Dissociation constant
1.23 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD168 also known as HMMR (hyaluronan-mediated motility receptor) is a protein that functions mechanically as a receptor involved in cell motility proliferation and adhesion. Its molecular weight is approximately 95 kDa. CD168 expression can be prominently found in tissues that contain a high density of hyaluronan such as tumor cells and certain types of epithelial cells. Additionally CD168 is often used as a marker for certain macrophage populations illustrating its role in the immune landscape.

Biological function summary

Hyaluronan-mediated motility receptor plays an important role in the regulation of the extracellular matrix (ECM) by binding to hyaluronan a significant component of the ECM. CD168 has a vital function in cell surface signaling processes facilitating communication between cells and their environment. The protein operates in a complex with elements like growth factor receptors enhancing its ability to modulate cellular responses. This interaction allows CD168 to significantly influence processes like wound healing and tissue remodeling.

Pathways

Hyaluronan-mediated motility receptor participates in the Rho signaling pathway important for cytoskeletal organization and cell movement. Through this pathway CD168 interacts directly with proteins such as GTPases including members of the Rho family like RhoA which play key roles in the reorganization of the actin cytoskeleton. CD168 also relates to the PI3K/AKT pathway influencing cellular survival and proliferation by interacting with proteins such as PI3K integrally involved in cellular growth and response to extracellular signals.

Associated diseases and disorders

Increased expression of hyaluronan-mediated motility receptor has been observed in several cancers including breast and ovarian cancers where it often correlates with advanced disease stages and poor prognosis. CD168's role in cancer is linked with its interaction with the epidermal growth factor receptor (EGFR) which promotes aggressive tumor behavior. In arthritis CD168 expression contributes to synovial inflammation connecting with inflammatory cytokines that escalate the disease progression. These associations underline the importance of CD168 in both oncology and inflammatory disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-CD168 antibody [EPR4054] (ab124729), expandable thumbnail

    Immunoprecipitation - Anti-CD168 antibody [EPR4054] (ab124729)

    ab124729 (purified) at 1:100 dilution (2ug) immunoprecipitating CD168 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2 (+): ab124729 & MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab124729 in MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CD168 antibody [EPR4054] (ab124729)

    Predicted band size: 84 kDa

    Observed band size: 85 kDa

  • Western blot - Anti-CD168 antibody [EPR4054] (ab124729), expandable thumbnail

    Western blot - Anti-CD168 antibody [EPR4054] (ab124729)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: Empty
    Lane 3: CD168 knockout HAP1 whole cell lysate (20 μg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab124729 observed at 90 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab124729 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and CD168 knockout samples were subjected to SDS-PAGE. ab124729 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD168 antibody [EPR4054] (ab124729)

    Predicted band size: 84 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] (ab124729), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] (ab124729)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric carcinoma tissue sections labeling CD168 with purified ab124729 at 1:250 dilution (7.7 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] (ab124729), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4054] (ab124729)

    Immunohistochemical analysis of paraffin-embedded human testis tissue using unpurified ab124729 at 1/50 - 1/100 dilution

  • Western blot - Anti-CD168 antibody [EPR4054] (ab124729), expandable thumbnail

    Western blot - Anti-CD168 antibody [EPR4054] (ab124729)

    5% NFDM/TBST

    All lanes: Western blot - Anti-CD168 antibody [EPR4054] (ab124729) at 1/1000 dilution

    Lane 1: MDA-MB-435 (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 20 µg

    Lane 2: RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 125 kDa, 84 kDa

  • Western blot - Anti-CD168 antibody [EPR4054] (ab124729), expandable thumbnail

    Western blot - Anti-CD168 antibody [EPR4054] (ab124729)

    All lanes: Western blot - Anti-CD168 antibody [EPR4054] (ab124729)

    Lane 1: MDA-MB-435 cell lysate at 10 µg

    Lane 2: LnCaP cell lysate at 10 µg

    Lane 3: T47-D cell lysate at 10 µg

    Predicted band size: 84 kDa

  • OI-RD Scanning - Anti-CD168 antibody [EPR4054] (ab124729), expandable thumbnail

    OI-RD Scanning - Anti-CD168 antibody [EPR4054] (ab124729)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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