Anti-CD168 antibody [EPR4055]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 at 1/100 staining CD168 in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 showing positive staining in Normal stomach tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 showing positive staining in Normal thymus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 showing positive staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 at 1/100 staining CD168 in paraffin-embedded human testis tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Lab

Immunoprecipitation - Anti-CD168 antibody [EPR4055] (AB108339)

Purified ab108339 at 1/50 dilution (2μg) immunoprecipitating CD168 in T-47D whole cell lysate.
Lane 1 (input) : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab108339 + T-47D whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108339 in T-47D whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 85 kDa

All lanes:

Immunoprecipitation - Anti-CD168 antibody [EPR4055] (ab108339)

Predicted band size: 84 kDa

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• WB

Unknown

Western blot - Anti-CD168 antibody [EPR4055] (AB108339)

All lanes:

Western blot - Anti-CD168 antibody [EPR4055] (ab108339) at 1/1000 dilution

Lane 1:

T47-D cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

SKBR-3 cell lysate at 10 µg

Lane 4:

LnCaP cell lysate at 10 µg

Predicted band size: 84 kDa

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• WB

Lab

Western blot - Anti-CD168 antibody [EPR4055] (AB108339)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Empty knockout HAP1 whole cell lysate (20 μg)
Lane 3 : CD168 whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - ab108339 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab108339 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and Empty knockout samples were subjected to SDS-PAGE. ab108339 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD168 antibody [EPR4055] (ab108339)

Predicted band size: 84 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD168 antibody [EPR4055] (AB108339)

ab108339 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

CiteAb

Western blot - Anti-CD168 antibody [EPR4055] (AB108339)

CD168 western blot using anti-CD168 antibody [EPR4055] ab108339. Publication image and figure legend from Picaud, L., Thibault, B., et al., 2014, J Ovarian Res, PubMed 24739440.

ab108339 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108339 please see the product overview.

CD44 and CD168 expression in ovarian cancer cell lines. (A) The CD44 expression was measured by flow cytometry and analyzed with Cell Quest Pro software in ovarian cancer cell lines OVCAR-3, IGROV-1 and SKOV-3 as well as in REH lymphoblasts (negative control) and HeLa cells (positive controls). The auto fluorescence level was determined with an isotype control antibody (empty area). The CD44 expression was determined with an anti-CD44 antibody (full area) leading to the determination of the percentage of CD44 positive cells (M1). (B) The CD168 level was determined by western blot using a specific antibody in ovarian cancer cell lines OVCAR-3, IGROV-1 and SKOV-3 as well as in REH lymphoblasts and HeLa (positive controls). Immunocomplexes were visualized with a photon camera.

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