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Rabbit Recombinant Monoclonal CD16a antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-CD16a antibody [SP189] - BSA and Azide free (AB243936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (AB243936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (AB243936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (AB243936), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (AB243936), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at
room temperature for 20 minutes.

Associated Products

Select an associated product type

15 products for Alternative Product

9 products for Alternative Version

Target data

Function

Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Alternative names

Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma RIII-alpha, FcR-10, IgG Fc receptor III-2, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FCGR3A, FCGR3, FCG3, IGFR3, CD16A

Recommended products

Rabbit Recombinant Monoclonal CD16a antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

SP189

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab243936 is the carrier-free version of Anti-CD16a antibody [SP189] - C-terminal ab227665.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The CD16a protein also known as Fc gamma receptor IIIa (FcγRIIIa) plays an important role in the immune system. This receptor is about 50-70 kDa in mass and is expressed on the surface of natural killer (NK) cells macrophages and some T cells. CD16a facilitates antibody-dependent cellular cytotoxicity (ADCC) by binding to the Fc region of immunoglobulin G (IgG). This interaction allows effector immune cells to recognize and destroy antibody-coated target cells.

Biological function summary

CD16a is important for immune responses and occurs as part of a receptor complex. It signals downstream effects through its association with the CD3 zeta chain and the Fc epsilon receptor gamma chain which possess immunoreceptor tyrosine-based activation motifs (ITAMs). Upon binding to IgG CD16a triggers a cascade of events leading to the release of cytokines and cytotoxic granules. This action helps in eliminating infected or cancerous cells demonstrating its role in both innate and adaptive immunity.

Pathways

CD16a contributes significantly to the immunological synapse formation and NK cell activation pathway. This receptor cooperates with other proteins such as CD3 and CD19 to modulate the immune response. It also participates in signaling pathways like the PI3K-Akt pathway which regulates cell survival and proliferation. CD16a's interaction with these pathways highlights its importance in immune cell communication and function.

Associated diseases and disorders

CD16a is linked to autoimmune diseases and certain cancers. In autoimmune disorders like systemic lupus erythematosus (SLE) abnormal CD16a function can contribute to the pathogenesis by altering immune cell targeting. Additionally in cancers such as lymphoma CD16a's interaction with IgG-coated cancer cells can influence the efficacy of monoclonal antibody therapies. The receptor's association with proteins like CD3 and CD19 in these disease contexts highlights its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Flow Cytometry (Intracellular) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 (green) compared with a rabbit isotype control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution (1.47 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution (1.47 μg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human liver tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16 with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antiody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - BSA and Azide free (ab243936)

    Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD16a with Anti-CD16a antibody [SP189] - C-terminal ab227665 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD16a antibody [SP189] - C-terminal ab227665).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com