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Rabbit Recombinant Monoclonal CD16a antibody. C-terminal. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (AB228278), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (AB228278), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (AB228278), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (AB228278), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (AB228278), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/1

Notes

Ready to use.

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Associated Products

Select an associated product type

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7 products for Alternative Version

Target data

Function

Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Alternative names

Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma RIII-alpha, FcR-10, IgG Fc receptor III-2, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FCGR3A, FCGR3, FCG3, IGFR3, CD16A

Recommended products

Rabbit Recombinant Monoclonal CD16a antibody. C-terminal. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP189

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The CD16a protein also known as Fc gamma receptor IIIa (FcγRIIIa) plays an important role in the immune system. This receptor is about 50-70 kDa in mass and is expressed on the surface of natural killer (NK) cells macrophages and some T cells. CD16a facilitates antibody-dependent cellular cytotoxicity (ADCC) by binding to the Fc region of immunoglobulin G (IgG). This interaction allows effector immune cells to recognize and destroy antibody-coated target cells.

Biological function summary

CD16a is important for immune responses and occurs as part of a receptor complex. It signals downstream effects through its association with the CD3 zeta chain and the Fc epsilon receptor gamma chain which possess immunoreceptor tyrosine-based activation motifs (ITAMs). Upon binding to IgG CD16a triggers a cascade of events leading to the release of cytokines and cytotoxic granules. This action helps in eliminating infected or cancerous cells demonstrating its role in both innate and adaptive immunity.

Pathways

CD16a contributes significantly to the immunological synapse formation and NK cell activation pathway. This receptor cooperates with other proteins such as CD3 and CD19 to modulate the immune response. It also participates in signaling pathways like the PI3K-Akt pathway which regulates cell survival and proliferation. CD16a's interaction with these pathways highlights its importance in immune cell communication and function.

Associated diseases and disorders

CD16a is linked to autoimmune diseases and certain cancers. In autoimmune disorders like systemic lupus erythematosus (SLE) abnormal CD16a function can contribute to the pathogenesis by altering immune cell targeting. Additionally in cancers such as lymphoma CD16a's interaction with IgG-coated cancer cells can influence the efficacy of monoclonal antibody therapies. The receptor's association with proteins like CD3 and CD19 in these disease contexts highlights its potential as a therapeutic target.

Product promise

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Full details and terms and conditions can be found here:
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11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human lung tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human liver tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16 using ab228278 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - C-terminal, prediluted (ab228278)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16 using ab228278 in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com