AB246798

Anti-CD16a antibody [SP189] - Low endotoxin, Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD16a antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD16a, CD16A, FCG3, FCGR3, IGFR3, FCGR3A, Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, FcR-10, IgG Fc receptor III-2, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human liver tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

Flow Cytometry (Intracellular) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD16a with ab227665 at 1/100 (green) compared with a rabbit isotype control (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227665).

Unconjugated

Anti-CD16a antibody [SP189] - C-terminal
Carrier free

Anti-CD16a antibody [SP189] - BSA and Azide free
578 PE

PE Anti-CD16 antibody [SP189] (C-terminal)
660 APC

APC Anti-CD16 antibody [SP189] (C-terminal)
HRP

HRP Anti-CD16 antibody [SP189] (C-terminal)
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD16 antibody [SP189] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD16 antibody [SP189] - C-terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD16 antibody [SP189] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD16 antibody [SP189] - C-terminal
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD16 antibody [SP189] - C-terminal
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD16a antibody [SP189] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP189

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab246798 is the carrier-free version of ab227665.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Endotoxin level is less than 1 EU/ml as determined by the TAL test

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CD16a protein also known as Fc gamma receptor IIIa (FcγRIIIa) plays an important role in the immune system. This receptor is about 50-70 kDa in mass and is expressed on the surface of natural killer (NK) cells macrophages and some T cells. CD16a facilitates antibody-dependent cellular cytotoxicity (ADCC) by binding to the Fc region of immunoglobulin G (IgG). This interaction allows effector immune cells to recognize and destroy antibody-coated target cells.

Biological function summary

CD16a is important for immune responses and occurs as part of a receptor complex. It signals downstream effects through its association with the CD3 zeta chain and the Fc epsilon receptor gamma chain which possess immunoreceptor tyrosine-based activation motifs (ITAMs). Upon binding to IgG CD16a triggers a cascade of events leading to the release of cytokines and cytotoxic granules. This action helps in eliminating infected or cancerous cells demonstrating its role in both innate and adaptive immunity.

Pathways

CD16a contributes significantly to the immunological synapse formation and NK cell activation pathway. This receptor cooperates with other proteins such as CD3 and CD19 to modulate the immune response. It also participates in signaling pathways like the PI3K-Akt pathway which regulates cell survival and proliferation. CD16a's interaction with these pathways highlights its importance in immune cell communication and function.

CD16a is linked to autoimmune diseases and certain cancers. In autoimmune disorders like systemic lupus erythematosus (SLE) abnormal CD16a function can contribute to the pathogenesis by altering immune cell targeting. Additionally in cancers such as lymphoma CD16a's interaction with IgG-coated cancer cells can influence the efficacy of monoclonal antibody therapies. The receptor's association with proteins like CD3 and CD19 in these disease contexts highlights its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger (PubMed : 11711607, PubMed : 21768335, PubMed : 22023369, PubMed : 24412922, PubMed : 25786175, PubMed : 25816339, PubMed : 28652325, PubMed : 8609432, PubMed : 9242542). Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. Involved in the generation of memory-like adaptive NK cells capable to produce high amounts of IFNG and to efficiently eliminate virus-infected cells via ADCC (PubMed : 24412922, PubMed : 25786175). Regulates NK cell survival and proliferation, in particular by preventing NK cell progenitor apoptosis (PubMed : 29967280, PubMed : 9916693). Following the engagement of antigen-IgG complexes, triggers phosphorylation of immunoreceptor tyrosine-based activation motif (ITAM)-containing adapters with subsequent activation of phosphatidylinositol 3-kinase signaling and sustained elevation of intracellular calcium that ultimately drive NK cell activation. The ITAM-dependent signaling coupled to receptor phosphorylation by PKC mediates robust intracellular calcium flux that leads to production of pro-inflammatory cytokines, whereas in the absence of receptor phosphorylation it mainly activates phosphatidylinositol 3-kinase signaling leading to cell degranulation (PubMed : 1825220, PubMed : 23024279, PubMed : 2532305). Costimulates NK cells and trigger lysis of target cells independently of IgG binding (PubMed : 10318937, PubMed : 23006327). Mediates the antitumor activities of therapeutic antibodies. Upon ligation on monocytes triggers TNFA-dependent ADCC of IgG-coated tumor cells (PubMed : 27670158). Mediates enhanced opsonisation and ADCC in response to afucosylated IgGs (PubMed : 34485821, PubMed : 28566370).. (Microbial infection) Involved in Dengue virus pathogenesis via antibody-dependent enhancement (ADE) mechanism. Secondary infection with Dengue virus triggers elevated levels of afucosylated non-neutralizing IgG1s with reactivity to viral envelope/E protein. Viral antigen-IgG1 complexes bind with high affinity to FCGR3A, facilitating virus entry in myeloid cells and subsequent viral replication.

See full target information FCGR3A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 17:268 PubMed31412888

2019

B7-H3 participates in human salivary gland epithelial cells apoptosis through NF-κB pathway in primary Sjögren's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Ying Yang,Yi Jin,Rui Zhao,Chen Dong,Wenjie Zheng,Tianyi Zhang,Jing Li,Zhifeng Gu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD16a antibody [SP189] - Low endotoxin, Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Flow Cytometry (Intracellular) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16a antibody [SP189] - Low endotoxin, Azide free (AB246798)

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Supplementary information

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Publications (1)

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