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AB89207

Anti-CD16b antibody [MM0272-5L11]

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(3 Publications)

Mouse Monoclonal CD16b antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FCGR3B.

View Alternative Names

CD16b, CD16B, FCG3, FCGR3, IGFR3, FCGR3B, Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, FcR-10, IgG Fc receptor III-1, CD16-I, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb

2 Images
Western blot - Anti-CD16b antibody [MM0272-5L11] (AB89207)
  • WB

Supplier Data

Western blot - Anti-CD16b antibody [MM0272-5L11] (AB89207)

All lanes:

Western blot - Anti-CD16b antibody [MM0272-5L11] (ab89207) at 1/500 dilution

All lanes:

Human placenta tissue lysate

Predicted band size: 26 kDa

Observed band size: 23 kDa

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Western blot - Anti-CD16b antibody [MM0272-5L11] (AB89207)
  • WB

Supplier Data

Western blot - Anti-CD16b antibody [MM0272-5L11] (AB89207)

All lanes:

Western blot - Anti-CD16b antibody [MM0272-5L11] (ab89207) at 1/500 dilution

All lanes:

Human embryonic tissue lysate

Predicted band size: 26 kDa

Observed band size: 23 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0272-5L11

Isotype

IgG2

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human FCGR3B. The exact immunogen used to generate this antibody is proprietary information.

O75015

Specificity

In recent direct ELISA testing, we found that this antibody cross-reacts ~50% with Human Fc gamma RIIIA/CD16a.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/mouse-igg2a-kappa-monoclonal-mg2a-53-low-endotoxin-azide-free-isotype-control-ab18414'>ab18414</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Reconstituted antibody should be aliquoted and can be stored frozen at < -20°C for at least for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with PBS at 200µL
Purification technique
Affinity purification Protein G
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-80°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD16b also known as FCGR3B is a low-affinity receptor for the Fc region of IgG with a molecular weight of around 50-65 kDa. It is a glycosylphosphatidylinositol (GPI)-anchored protein expressed specifically on the surface of human neutrophils. CD16b plays a role in antibody-dependent cellular cytotoxicity (ADCC) by interacting with immune complexes and its expression can be detected by ELISA. This protein shares similarities with CD16a which is expressed on NK cells and macrophages but they differ in their cellular distribution and functions.
Biological function summary

CD16b functions as an important receptor in the immune response by facilitating interactions between neutrophils and opsonized pathogens. This protein does not operate in isolation but works with other components of the immune system to trigger a sequence of reactions leading to the destruction of target cells and pathogens. The engagement of CD16b initiates signal transduction pathways that activate effector functions including phagocytosis and the release of inflammatory mediators. It helps the immune system in removing pathogens efficiently.

Pathways

CD16b integrates into signaling pathways that contribute to immune defense. It is part of the Fc gamma R-mediated phagocytosis pathway which involves the collaboration with spleen tyrosine kinase (Syk) and Src family kinases. This pathway is critical in phagocytosis a process by which cells engulf and destroy pathogens. CD16b associates with other Fc gamma receptors such as CD32 to adjust and amplify the immune response allowing for targeted immune activity in detecting and eliminating infected or altered cells.

CD16b connects to autoimmune and inflammatory conditions. Altered CD16b expression or function may associate with diseases like systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). These conditions can involve dysregulation of immune complexes and Fc receptor pathways including those influenced by CD32 contributing to the pathogenic process. In these diseases CD16b might play a role in the pathogenic autoimmune response by influencing the clearance of immune complexes and modulating inflammatory processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
See full target information FCGR3B
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 25:70 PubMed39987176

2025

Compound kushen injection improves radiation enteritis via cannabinoid receptor 1 in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Xu,Liping Gao,Wenjuan Zou,Xiaohui Tang,Weiqi Nian,Weiqin Zheng,Rongzhong Huang,Pei Wang

Nature immunology 22:1107-1117 PubMed34385713

2021

Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Pengchong Li,Mengdi Jiang,Ketian Li,Hao Li,Yangzhong Zhou,Xinyue Xiao,Yue Xu,Suzanne Krishfield,Peter E Lipsky,George C Tsokos,Xuan Zhang

American journal of physiology. Heart and circulatory physiology 319:H730-H743 PubMed32795184

2020

Endothelial deficiency of insulin-like growth factor-1 receptor reduces endothelial barrier function and promotes atherosclerosis in -deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Higashi,Sergiy Sukhanov,Shaw-Yung Shai,Svitlana Danchuk,Patricia Snarski,Zhaohui Li,Xuwei Hou,Milton H Hamblin,T Cooper Woods,Meifang Wang,Derek Wang,Hong Yu,Ronald J Korthuis,Tadashi Yoshida,Patrice Delafontaine
View all publications
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