Rat Monoclonal FCGR3 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, BL and reacts with Mouse, Human samples. Cited in 17 publications.
Images
![Flow Cytometry - Anti-CD16+CD32 antibody [93] - BSA and Azide free (AB25235), expandable thumbnail](https://content.abcam.com/products/images/cd16cd32-antibody-93-bsa-and-azide-free-ab25235--flow-cytometry-img105918.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD16+CD32 antibody [93] - BSA and Azide free (AB25235)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [93] - BSA and Azide free (AB25235), expandable thumbnail](https://content.abcam.com/products/images/cd16cd32-antibody-93-bsa-and-azide-free-ab25235--immunocytochemistry-immunofluorescence-img174728.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [93] - BSA and Azide free (AB25235)")
Publications
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- Journal of neuroinflammation 20:2442023ASK1-K716R reduces neuroinflammation and white matter injury via preserving blood-brain barrier integrity after traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Meng,Hui Cao,Yichen Huang,Ziyu Shi,Jiaying Li,Yana Wang,Yue Zhang,Shuning Chen,Hong Shi,Yanqin GaoPubMed 37875988
- Aging 15:2705-27202023Berberine mitigates intracerebral hemorrhage-induced neuroinflammation in a gut microbiota-dependent manner in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Liu,Haotian Chen,Ting Yu,Xiongjie Fu,Cong Qian,Xiuqin FengPubMed 37036515
- Neurotoxicity research 40:1393-14042022Polydatin Prevents Neuroinflammation and Relieves Depression via Regulating Sirt1/HMGB1/NF-κB Signaling in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHetao Bian,Ling Xiao,Liang Liang,Yinping Xie,Huiling Wang,Mark Slevin,Wen-Jun Tu,Gaohua WangPubMed 35986876
- Frontiers in neuroscience 15:7013172021Raloxifene Modulates Microglia and Rescues Visual Deficits and Pathology After Impact Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcia G Honig,Nobel A Del Mar,Desmond L Henderson,Dylan O'Neal,John B Doty,Rachel Cox,Chunyan Li,Aaron M Perry,Bob M Moore,Anton ReinerPubMed 34776838
- Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 17:350-3662021Intranasal Salvinorin A Improves Long-term Neurological Function via Immunomodulation in a Mouse Ischemic Stroke Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDilidaer Misilimu,Wei Li,Di Chen,Pengju Wei,Yichen Huang,Sicheng Li,John Grothusen,Yanqin GaoPubMed 34596819
- Journal of neuroinflammation 18:432021Ceria nanoparticles ameliorate white matter injury after intracerebral hemorrhage: microglia-astrocyte involvement in remyelination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwei Zheng,Jia'nan Lu,Shuhao Mei,Haijian Wu,Zeyu Sun,Yuanjian Fang,Shenbin Xu,Xiaoyu Wang,Ligen Shi,Weilin Xu,Sheng Chen,Jun Yu,Feng Liang,Jianmin ZhangPubMed 33588866
- Journal of neuroinflammation 18:22021Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaijian Wu et. al.PubMed 33402181
- OncoTargets and therapy 13:1011-10252020The Ectopic Expression of SurvivinT34A and FilC Can Enhance the Oncolytic Effects of Vaccinia Virus in Murine Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinglong Wang,Yanxi Luo,Ting Sun,Chenyu Mao,Yili Jiang,Xiongfei Yu,Zhongqi Li,Tian Xie,Fusheng Wu,Hui Yan,Lisong TengPubMed 32099404
- Aging 11:12097-121132019Aerosol inhalation of a hydrogen-rich solution restored septic renal function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeifeng Yao et. al.PubMed 31841441
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 40:1048-10602019Extracellular ASC exacerbated the recurrent ischemic stroke in an NLRP3-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Fei He,Yi-Xuan Zeng,Ge Li,Yu-Kun Feng,Cheng Wu,Feng-Yin Liang,Yu Zhang,Yue Lan,Guang-Qing Xu,Zhong PeiPubMed 31216943
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | BL | |
|---|---|---|---|
| Human | Expected | Expected | Expected |
| Mouse | Tested | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
BL
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab25235 can be used for blocking of Fc gamma receptors. |
Species Mouse | Dilution info - | Notes ab25235 can be used for blocking of Fc gamma receptors. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b (PubMed:17558411). Mediates neutrophil activation by IgG complexes redundantly with Fcgr4 (PubMed:18097064).
Additional Targets
Fcgr2
Alternative names
CD16, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, Fcgr3
Recommended products
Rat Monoclonal FCGR3 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt, BL and reacts with Mouse, Human samples. Cited in 17 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 93
- Purification technique
- Affinity purification Protein G
- Epitope
- ab25235 reacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Do Not Freeze
Notes
This product was changed from ascites to tissue culture supernatant on 02/08/2019. Lot numbers higher than GR3269503 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD16 and CD32 also referred to as FCγRIII and FCγRII are proteins expressed on the surface of various immune cells like natural killer cells macrophages and some subsets of T cells. CD16 has a mass ranging between 50-80 kDa while CD32 exhibits variability in size between 32-40 kDa due to different isoforms. These proteins play pivotal roles in immune response by binding to the Fc region of immunoglobulins influencing cell activation and signaling.
- Biological function summary
CD16 and CD32 serve important roles in mediating immune cell interactions. They work as part of immune synapses facilitating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis processes. Due to their involvement in these complex activities they are included in immunological synapse formation and modulation extending their influence over overall immune regulation and response. These proteins are known to contribute to both innate and adaptive immune mechanisms as receptors for the Fc portion of immunoglobulin G.
- Pathways
CD16 and CD32 interact within signaling pathways like the Fc gamma receptor-mediated phagocytosis pathway and the immune response-regulating signaling pathway. These receptors work in coordination with other immunoglobulin family members to initiate targeted cellular reactions necessary for immune challenge responses. CD32 often partners with CD19 and CD21 within pathways to modulate B cell receptor-mediated signaling.
- Associated diseases and disorders
CD16 and CD32 have connections to autoimmune diseases and infectious diseases. Aberrant expression or dysfunction in these proteins can contribute to the development of conditions such as systemic lupus erythematosus where improper immune complex clearance occurs and chronic inflammation results. CD16's involvement with NK cells can also play a role in viral infection control highlighting their importance in pathogen defense mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CD16+CD32 antibody [93] - BSA and Azide free (ab25235), expandable thumbnail](https://content.abcam.com/products/images/cd16cd32-antibody-93-bsa-and-azide-free-ab25235--flow-cytometry-img105918.jpg "Flow Cytometry - Anti-CD16+CD32 antibody [93] - BSA and Azide free (ab25235)")
Flow Cytometry - Anti-CD16+CD32 antibody [93] - BSA and Azide free (ab25235)
C57BL/6 mouse splenocytes stained with ab25235 (right) or Rat IgG2aκ (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab25235) or Rat IgG2aκ (Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control ab18450) (1x106 in 100μl at 5 μg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD19 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
This image was generated using the ascites version of the product.
![Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [93] - BSA and Azide free (ab25235), expandable thumbnail](https://content.abcam.com/products/images/cd16cd32-antibody-93-bsa-and-azide-free-ab25235--immunocytochemistry-immunofluorescence-img174728.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [93] - BSA and Azide free (ab25235)")
Immunocytochemistry/ Immunofluorescence - Anti-CD16+CD32 antibody [93] - BSA and Azide free (ab25235)
ab25235 staining CD16 + CD32 in Raw264.7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab25235 at 10µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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