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Rabbit Recombinant Monoclonal CD177 antibody. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (AB255296), expandable thumbnail
  • Western blot - Anti-CD177 antibody [EPR22813-205] (AB255296), expandable thumbnail
  • Western blot - Anti-CD177 antibody [EPR22813-205] (AB255296), expandable thumbnail
  • Western blot - Anti-CD177 antibody [EPR22813-205] (AB255296), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (AB255296), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).

Alternative names

CD177, NB1, PRV1, UNQ595/PRO1181, CD177 antigen, Human neutrophil alloantigen 2a, NB1 glycoprotein, Polycythemia rubra vera protein 1, HNA-2a, NB1 GP, PRV-1

Recommended products

Rabbit Recombinant Monoclonal CD177 antibody. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22813-205
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD177 also known as neutrophil antigen NB1 is a glycoprotein with an approximate mass of 58 kDa. It is primarily expressed on the surface of neutrophils a type of white blood cell involved in immune response. The expression of CD177 can vary among individuals where some people might have a higher or lower presence on their neutrophils. CD177 has been identified to play a role in cell adhesion and signaling functions contributing to various cellular processes within the immune system.

Biological function summary

CD177 functions by mediating interactions between neutrophils and endothelial cells during inflammation. This protein is not known to be part of a multi-protein complex but it plays a significant role in facilitating neutrophil transmigration from blood vessels to sites of tissue damage or infection. This process supports the body's defense mechanisms by enabling neutrophils to quickly respond to external threats and participate in the inflammatory response.

Pathways

The involvement of CD177 in chemotactic activity and cell migration highlights its contribution to both the immune response and inflammation pathways. Its interaction with proteins such as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) enhances neutrophil transmigration across the endothelium. CD177 acts within these pathways to ensure a precise and rapid movement of neutrophils which is essential for an effective immune response.

Associated diseases and disorders

CD177 levels have associations with conditions like polycythemia vera and anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis. In polycythemia vera CD177 can affect the proliferation of blood cells due to its role in neutrophil function. In ANCA-associated vasculitis CD177's interaction with proteins such as PR3 (Proteinase 3) on the surface of neutrophils could contribute to the pathophysiology of the disease leading to inappropriate activation of the immune system and subsequent tissue damage.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)

    Immunohistochemical analysis of paraffin-embedded human bone marrow labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human bone marrow (PMID: 22434870). The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296), expandable thumbnail

    Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)

    CD177 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 17580308).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 secs; Lane 2: 3 mins.

    All lanes: Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/1000 dilution

    Lane 1: Human lung lysate at 10 µg

    Lane 2: Human spleen lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa

  • Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296), expandable thumbnail

    Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)

    CD177 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 17580308). Negative control: U-937 (PMID: 17580308).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/1000 dilution

    Lane 1: Human liver lysate at 20 µg

    Lane 2: U-937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa

    Exposure time: 3min

  • Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296), expandable thumbnail

    Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD177 antibody [EPR22813-205] (ab255296) at 1/5000 dilution

    All lanes: Recombinant his-tagged human CD177 protein (aa22-407), 20 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 46 kDa

    Exposure time: 59s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on neutrophils in human spleen (PMID: 24562309) is observed. The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD177 antibody [EPR22813-205] (ab255296)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD177 with ab255296 at 1/2000 dilution (0.252 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on neutrophils in human placenta. The section was incubated with ab255296 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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