AB119347

Anti-CD18 antibody [2E6]

Name: Anti-CD18 antibody [2E6] (ab119347) | Abcam
Brand: Abcam Limited
SKU: AB119347
Price: 500 USD
Availability: InStock

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(4 Publications)

Hamster Monoclonal CD18 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human ITGB2 aa 1-50.

View Alternative Names

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

1 Images

Flow Cytometry - Anti-CD18 antibody [2E6] (AB119347)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD18 antibody [2E6] (AB119347)

Flow cytometry analysis of CD18 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (ab119347) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.

Key facts

Host species

Hamster

Clonality

Monoclonal

Clone number

2E6

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human ITGB2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P05107

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/armenian-hamster-igg-monoclonal-htk888-isotype-control-low-endotoxin-azide-free-ab18474'>ab18474</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 99% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.

Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407).

See full target information ITGB2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

American journal of respiratory and critical care medicine 166:53-60 PubMed12091171

2002

Very late antigen-4 in CD18-independent neutrophil emigration during acute bacterial pneumonia in mice.

Applications

Species

Mouse

Sadatomo Tasaka,Sarah E Richer,Joseph P Mizgerd,Claire M Doerschuk

The Journal of cell biology 152:857-66 PubMed11238444

2001

alpha(4)-integrin mediates neutrophil-induced free radical injury to cardiac myocytes.

Applications

Species

Mouse

B Y Poon,C A Ward,C B Cooper,W R Giles,A R Burns,P Kubes

Toxicological sciences : an official journal of the Society of Toxicology 54:509-16 PubMed10774834

2000

The hepatic inflammatory response after acetaminophen overdose: role of neutrophils.

Applications

Species

Mouse

J A Lawson,A Farhood,R D Hopper,M L Bajt,H Jaeschke

Circulation research 84:1245-51 PubMed10364561

1999

Emigrated neutrophils regulate ventricular contractility via alpha4 integrin.

Applications

BL, Flow Cyt

Species

Mouse, Mouse

B Y Poon,C A Ward,W R Giles,P Kubes

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CD18 antibody [2E6]

Flow Cytometry - Anti-CD18 antibody [2E6] (AB119347)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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