JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131044

Anti-CD18 antibody

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Polyclonal CD18 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human ITGB2.

View Alternative Names

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 antibody (AB131044)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 antibody (AB131044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Human Mammary Cancer Tissue at 1μg/ml.

Western blot - Anti-CD18 antibody (AB131044)
  • WB

Unknown

Western blot - Anti-CD18 antibody (AB131044)

All lanes:

Western blot - Anti-CD18 antibody (ab131044) at 0.1 µg/mL

Lane 1:

Jurkat cell lysate

Lane 2:

CEM cell lysate

Lane 3:

HT1080 cell lysate

Lane 4:

SMMc cell lysate

Lane 5:

HeLa cell lysate

Predicted band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ITGB2. The exact immunogen used to generate this antibody is proprietary information.

P05107

style
left-column

Alternative Products

Primary Antibodies

AB8220

Anti-CD18 antibody [MEM-148]

Flow Cytometry - Anti-CD18 antibody [MEM-148] (AB8220)

  • 4
  • 1
Primary Antibodies

AB657

Anti-CD18 antibody [MEM-48]

Flow Cytometry - Anti-CD18 antibody [MEM-48] (AB657)

  • 4
  • 1
Primary Antibodies

AB119830

Anti-CD18 antibody [M18/2]

Immunocytochemistry - Anti-CD18 antibody [M18/2] (AB119830)

  • 5
  • 1
Primary Antibodies

AB52920

Anti-CD18 (phospho S745) antibody [EP1288Y]

RabMAb|Recombinant

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

  • 0
  • 0
Primary Antibodies

AB232579

Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free (AB232579)

  • 0
  • 0

Complementary Products

Assay Kits

AB315433

Human CD18 ELISA Kit

SimpleStep

Sandwich ELISA - Human CD18 ELISA Kit (AB315433)

  • 0
  • 0
Primary Antibodies

AB13219

Anti-CD11+CD18 antibody [24]

  • 4
  • 5
Primary Antibodies

AB275964

Anti-ALK antibody [EPR24029-128]

RabMAb|Recombinant

Western blot - Anti-ALK antibody [EPR24029-128] (AB275964)

  • 0
  • 0
Primary Antibodies

AB233396

Anti-CD69 antibody [EPR21814]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD69 antibody [EPR21814] (AB233396)

  • 5
  • 4
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.
Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Integrin ITGAL : ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed : 1676048, PubMed : 23775590, PubMed : 38195629). Integrin ITGAL : ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM : ITGB2 and ITGAX : ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX : ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM : ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM : ITGB2 is also a receptor for factor X. Integrin ITGAD : ITGB2 is a receptor for ICAM3 and VCAM1 (PubMed : 10438935, PubMed : 8777714, PubMed : 9841932). Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL : ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrins ITGAX : ITGB2 functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis (PubMed : 16985175). Integrins ITGAX : ITGB2 functions as a receptor of the neuron-specific adhesion molecule ICAM5 ensuring neuron cell-leukocyte adhesion (PubMed : 10741396). Integrin ITGAL : ITGB2 functions as a receptor of ICAM1 by acting as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed : 38195629). Integrin ITGAM : ITGB2/MAC-1 complex functions as a signaling receptor for the ligand receptor ICAM1, ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). Integrin ITGAL/ITGB2 that functions as a signaling receptor of ICAM2, ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 1676048).
See full target information ITGB2
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 9:183-197 PubMed34820565

2021

Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Bao,Geng Dou,Ran Tian,Yajie Lv,Feng Ding,Siying Liu,Ruifeng Zhao,Lu Zhao,Jun Zhou,Lin Weng,Yan Dong,Bei Li,Shiyu Liu,Xin Chen,Yan Jin

Frontiers in medicine 8:674523 PubMed34355002

2021

Expression and Pathogenic Analysis of Integrin Family Genes in Systemic Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Xu,Ting Li,Ruikang Wang,Rong Mu

Journal of asthma and allergy 14:585-593 PubMed34079296

2021

Cell Adhesion Molecules are Upregulated and May Drive Inflammation in Chronic Rhinosinusitis with Nasal Polyposis.

Applications

Unspecified application

Species

Unspecified reactive species

Brennan J Blight,Amarbir S Gill,Jorgen S Sumsion,Chelsea E Pollard,Shaelene Ashby,Gretchen M Oakley,Jeremiah A Alt,Abigail Pulsipher

International journal of molecular sciences 19: PubMed29949925

2018

Physiological Hypoxia (Physioxia) Impairs the Early Adhesion of Single Lymphoma Cell to Marrow Stromal Cell and Extracellular Matrix. Optical Tweezers Study.

Applications

Unspecified application

Species

Unspecified reactive species

Kamila Duś-Szachniewicz,Sławomir Drobczyński,Piotr Ziółkowski,Paweł Kołodziej,Kinga M Walaszek,Aleksandra K Korzeniewska,Anil Agrawal,Piotr Kupczyk,Marta Woźniak

Cellular physiology and biochemistry : internation 46:36-45 PubMed29566360

2018

The Antimuscarinic Agent Tolterodine Regulates Bladder Extracellular Matrix in Partial Bladder Outlet Obstruction in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tong-Xin Yang,De-Yi Luo,Yi-Fei Lin,Qiang Liu,Xiang Cai,Jian-Zhong Ai,Hong Li,Hong Shen,Kun-Jie Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com