Anti-CD18 antibody [EPR26929-26] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling CD18 with ab307406 at 1/100 (5.22 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control : no staining on human cardiac muscle. The section was incubated with ab307406 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD18 with ab307406 at 1/100 (5.22 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human spleen. The section was incubated with ab307406 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling CD18 with ab307406 at 1/100 (5.22 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on (A) HEK-293T transfected with a ITGB2 his tag construct, no staining on (B) HEK-293T transfected with empty plasmid.The section was incubated with ab307406 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling CD18 with ab307406 at 1/100 (5.22 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on macrophages of human lung. The section was incubated with ab307406 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized U-937 (human histiocytic lymphoma monocyte) cells lABelling CD18 with ab307406 at 1/1000 (0.522 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in U-937 cell line.Negative control : HeLa.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt

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Flow Cytometry - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Flow cytometric analysis of HeLa (human cervix adenocarcinoma epithelial cell, Left) / U937 (human histiocytic lymphoma monocyte, Right) cells labelling CD18 with ab307406 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Negative control : HeLa (PMID : 31109948).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab307406 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Cells were stained with Rabbit IgG or ab307406. Then stained with anti-CD4 conjugated to Alexa Fluor^® 647.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using ab307406, the same antibody clone in a different buffer formulation. Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells lABelling CD18 with ab307406 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Cells were stained with Rabbit IgG or ab307406. Then stained with anti-CD14 conjugated to Alexa Fluor^® 647.

• WB

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Western blot - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using 307406, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : human heart The MW is consistent to what has been described in the literature (PMID : 31109948) Samples are non-boiled as boiling may cause protein aggregation. Lanes 1 : 37 seconds, Lanes 2-3 : 59 seconds, Lanes 4-5 : 103 seconds, Exposure time :

All lanes:

Western blot - Anti-CD18 antibody [EPR26929-26] (<a href='/en-us/products/primary-antibodies/cd18-antibody-epr26929-26-ab307406'>ab307406</a>) at 1/1000 dilution

Lane 1:

Human lymphoma tissue 20 μg

Lane 2:

Human heart tissue 20 μg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 90 kDa,110 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-CD18 antibody [EPR26929-26] - BSA and Azide free (AB307407)

This data was developed using 307406, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : Hela The MW is consistent to what has been described in the literature (PMID : 31109948) Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Samples are non-boiled as boiling may cause protein aggregation. 26 seconds Exposure time :

All lanes:

Western blot - Anti-CD18 antibody [EPR26929-26] (<a href='/en-us/products/primary-antibodies/cd18-antibody-epr26929-26-ab307406'>ab307406</a>) at 1/1000 dilution

Lane 1:

U-937 (human histiocytic lymphoma monocyte) whole cell lysate 20 μg

Lane 2:

Hela (human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa,110 kDa

false

Exposure time: 26s