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AB119830

Anti-CD18 antibody [M18/2]

5

(1 Review)

|

(11 Publications)

Rat Monoclonal CD18 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples. Cited in 11 publications. Immunogen corresponding to Full Length Protein corresponding to Mouse Itgb2.

View Alternative Names

CD18, Integrin beta-2, Complement receptor C3 subunit beta, Itgb2

3 Images
Immunocytochemistry - Anti-CD18 antibody [M18/2] (AB119830)
  • ICC

Collaborator

Immunocytochemistry - Anti-CD18 antibody [M18/2] (AB119830)

ICC/IF image of CD18 staining on RAW264.7 cells using ab119830 (1 : 300). The cells were fixed using formaldehyde and later permeabilized using 0.2% Triton X-100. The cells were blocked using 10% goat serum for 1 hour at 20°C. ab119830 was diluted to 1 : 300 and incubated with the cells for 1 hour at 20°C. The secondary antibody used was Anti-rat goat polyclonal conjugated to Alexa Fluor 647 (1 : 350).

This image is courtesy of an anonymous abreview.

Flow Cytometry - Anti-CD18 antibody [M18/2] (AB119830)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD18 antibody [M18/2] (AB119830)

Flow Cytometry analysis of mouse myeloid cells labeling CD18 with ab119830 at 16 μg/mL (red-filled).

Mouse myeloid cells unstained by primary antibody (DAR APC, black-dashed).

Flow Cytometry - Anti-CD18 antibody [M18/2] (AB119830)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD18 antibody [M18/2] (AB119830)

Flow cytometry analysis of mouse splenocytes labeling CD18 with ab119830 at 16 μg/mL.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M18/2

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Full Length Protein corresponding to Mouse Itgb2. The exact immunogen used to generate this antibody is proprietary information.

P11835

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-16 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Dog": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-16 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
> 95% (by SDS-PAGE)
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.
Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (By similarity). Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (By similarity). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (By similarity). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (By similarity). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). Integrin ITGAM/ITGB2 plays a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the ITGAM subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.
See full target information Itgb2
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34066088

2021

Alternative C3 Complement System: Lipids and Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Maisa Garcia-Arguinzonis,Elisa Diaz-Riera,Esther Peña,Rafael Escate,Oriol Juan-Babot,Pedro Mata,Lina Badimon,Teresa Padro

Cell death and differentiation 28:2728-2744 PubMed33934104

2021

PP2Acα promotes macrophage accumulation and activation to exacerbate tubular cell death and kidney fibrosis through activating Rap1 and TNFα production.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Xiaoli Sun,Mingjie Wang,Qingmiao Lu,Mengru Gu,Lu Zhou,Qing Hou,Mengzhu Tan,Sudan Wang,Xian Xue,Chunsun Dai

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33876755

2021

Immune cells fold and damage fungal hyphae.

Applications

Unspecified application

Species

Unspecified reactive species

Judith M Bain,M Fernanda Alonso,Delma S Childers,Catriona A Walls,Kevin Mackenzie,Arnab Pradhan,Leanne E Lewis,Johanna Louw,Gabriela M Avelar,Daniel E Larcombe,Mihai G Netea,Neil A R Gow,Gordon D Brown,Lars P Erwig,Alistair J P Brown

Experimental and therapeutic medicine 18:1953-1960 PubMed31410157

2019

Acute remote ischemic preconditioning alleviates free radical injury and inflammatory response in cerebral ischemia/reperfusion rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Liang Meng,Dong-Lin Zhang,Shi-Hua Sui

Journal of cellular and molecular medicine 23:3402-3416 PubMed30869196

2019

The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-κB p65 translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Sheng Liu,Li-Fen Wang,Xiao-Shen Cheng,Ya-Ni Huo,Xiao-Mei Ouyang,Lai-Ying Liang,Ying Lin,Jian-Feng Wu,Jian-Lin Ren,Bayasi Guleng

Journal of cellular and molecular medicine 21:3445-3452 PubMed28661042

2017

CD11b promotes the differentiation of osteoclasts induced by RANKL through the spleen tyrosine kinase signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxi Yang,Xiaoyong Chen,Zhao Yan,Qingsheng Zhu,Chongfei Yang

Translational psychiatry 7:e1120 PubMed28485733

2017

Maternal immune activation results in complex microglial transcriptome signature in the adult offspring that is reversed by minocycline treatment.

Applications

IHC

Species

Mouse

D Mattei,A Ivanov,C Ferrai,P Jordan,D Guneykaya,A Buonfiglioli,W Schaafsma,P Przanowski,W Deuther-Conrad,P Brust,S Hesse,M Patt,O Sabri,T L Ross,B J L Eggen,E W G M Boddeke,B Kaminska,D Beule,A Pombo,H Kettenmann,S A Wolf

PloS one 10:e0116270 PubMed25643152

2015

Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ∆133p53α mimic.

Applications

WB

Species

Mouse

Sonal Sawhney,Kylie Hood,Alisha Shaw,Antony W Braithwaite,Richard Stubbs,Noelyn A Hung,Janice A Royds,Tania L Slatter

Blood 111:3591-8 PubMed18216298

2008

Neutrophil-selective CD18 silencing using RNA interference in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Cullere,Michael Lauterbach,Naotake Tsuboi,Tanya N Mayadas

Journal of immunology (Baltimore, Md. : 1950) 174:3668-75 PubMed15749905

2005

Manifestations of inflammatory arthritis are critically dependent on LFA-1.

Applications

Unspecified application

Species

Unspecified reactive species

Gerald M Watts,Frank J M Beurskens,Ines Martin-Padura,Christie M Ballantyne,Lloyd B Klickstein,Michael B Brenner,David M Lee
View all publications
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