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AB176540

Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free

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(2 Publications)

Mouse Monoclonal CD18 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, FuncS and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing ITGB2 protein.

View Alternative Names

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

3 Images
Flow Cytometry - Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (AB176540)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (AB176540)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab176540.

Flow Cytometry - Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (AB176540)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (AB176540)

Separation of human monocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab176540.

Western blot - Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (AB176540)
  • WB

Supplier Data

Western blot - Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (AB176540)

The reactivity of MEM-148 antibody was assessed by comparing binding signals in a panel of various human cell samples. Western blotting analysis was performed on RIPA buffer extracts of THP-1, Kg1a, U937, HPB-ALL, Raji, Jurkat, leukocytes, and HeLa cells, mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with ab176540 (2 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). For multiplex fluorescent Western blot detection, mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red).

CD18 was detected at ~90-100 kDa in the respective cell lines.

All lanes:

Western blot - Anti-CD18 antibody [MEM148] - Low endotoxin, Azide free (ab176540) at 2 µg/mL

Lane 1:

THP-1

Lane 2:

Kg1a

Lane 3:

U937

Lane 4:

HPB-ALL

Lane 5:

Raji

Lane 6:

Jurkat

Lane 7:

Leukocytes

Lane 8:

HeLa

Secondary

All lanes:

IRDye 800CW Goat-anti-Mouse IgG

Observed band size: 90-100 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM148

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, FuncS, Flow Cyt

applications

Immunogen

Cell preparation containing ITGB2 protein. The exact immunogen used to generate this antibody is proprietary information.

P05107

Epitope

ab176540 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p>ab176540 is an excellent marker of activated myeloid cells.</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p>Non-reducing conditions.</p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>ab176540 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.</p>" }, "Pig": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>ab176540 is an excellent marker of activated myeloid cells.</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p>Non-reducing conditions.</p>", "FuncS-species-checked": "notRecommended", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>ab176540 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.</p>" } } }
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Product details

Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purity > 95% (by SDS-PAGE).
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.
Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM/ITGB2 is also a receptor for factor X. Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1. Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407).
See full target information ITGB2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology research 2024:6343757 PubMed38715844

2024

Bystander Effects and Profibrotic Interactions in Hepatic Stellate Cells during HIV and HCV Coinfection.

Applications

Unspecified application

Species

Unspecified reactive species

Cintia Cevallos,Patricio Jarmoluk,Franco Sviercz,Cinthya A M López,Rosa N Freiberger,M Victoria Delpino,Jorge Quarleri

The Journal of biological chemistry 287:19585-98 PubMed22511754

2012

Signal-dependent slow leukocyte rolling does not require cytoskeletal anchorage of P-selectin glycoprotein ligand-1 (PSGL-1) or integrin αLβ2.

Applications

Unspecified application

Species

Unspecified reactive species

Bojing Shao,Tadayuki Yago,Phillip A Coghill,Arkadiusz G Klopocki,Padmaja Mehta-D'souza,David W Schmidtke,William Rodgers,Rodger P McEver
View all publications
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