AB52920

Anti-CD18 (phospho S745) antibody [EP1288Y]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal CD18 phospho S745 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

5 Images

Unknown

Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

ab52920 (purified) at 1 : 50 dilution (2μg) immunoprecipitating CD18 in Jurkat whole cell lysate.
Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab52920 & Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52920 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920)

Predicted band size: 84 kDa

false

Unknown

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/mL

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 84 kDa

false

Unknown

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/mL

Lane 1:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 15 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates 15ug. Then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 84 kDa

false

Unknown

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 0.9 µg/mL

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates. Then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 84 kDa

false

Unknown

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

All lanes:

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (ab52920) at 1/500 dilution

Lane 1:

Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Lane 2:

AP-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 84 kDa

Observed band size: 85 kDa

false

Carrier free

Anti-CD18 (phospho S745) antibody [EP1288Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1288Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD18 also known as ITGB2 is a protein with a molecular weight of approximately 95 kDa. It is part of the beta-2 integrin family and partners with different alpha subunits to form heterodimers. CD18 is primarily expressed on leukocytes including neutrophils monocytes macrophages and lymphocytes. As a surface marker CD18 plays a significant role in immune cell adhesion and migration enabling cells to respond to inflammation signals effectively.

Biological function summary

The CD18 protein functions in conjunction with associated alpha subunit partners forming a part of the wider integrin family complex. ItgB2 as it is sometimes referred to is essential for cell adhesion processes. These heterodimers facilitate binding between leukocytes and various ligands on endothelial cells which subsequently controls leukocyte transmigration through vascular walls. CD18's activities are critical in immune system responses making it a prominent focus in immunological research.

Pathways

The role of CD18 extends into inflammatory and immune response signaling pathways. It contributes critically to the leukocyte adhesion cascade. This cascade engagement involves interaction with other proteins such as ICAM-1 on endothelial surfaces. CD18 through its association in integrin-mediated signaling influences processes like phagocytosis and lymphocyte activation giving it a central place in cellular immunity.

Deficiencies or mutations affecting CD18 function correlate with disorders such as Leukocyte Adhesion Deficiency type I (LAD-I). This condition results from impaired leukocyte function due to defective integrin expression. The role of CD18 in arthritis is also significant primarily by mediating leukocyte infiltration into inflamed tissues. Its interaction with proteins like ICAM-1 becomes exceptionally relevant in these disease dynamics indicating potential therapeutic targets for modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin ITGAL : ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed : 1676048, PubMed : 23775590, PubMed : 38195629). Integrin ITGAL : ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Integrins ITGAM : ITGB2 and ITGAX : ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin ITGAX : ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin ITGAM : ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin ITGAM : ITGB2 is also a receptor for factor X. Integrin ITGAD : ITGB2 is a receptor for ICAM3 and VCAM1 (PubMed : 10438935, PubMed : 8777714, PubMed : 9841932). Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992, PubMed : 28807980). Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation (PubMed : 18587400). Integrin ITGAL : ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrins ITGAX : ITGB2 functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis (PubMed : 16985175). Integrins ITGAX : ITGB2 functions as a receptor of the neuron-specific adhesion molecule ICAM5 ensuring neuron cell-leukocyte adhesion (PubMed : 10741396). Integrin ITGAL : ITGB2 functions as a receptor of ICAM1 by acting as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed : 38195629). Integrin ITGAM : ITGB2/MAC-1 complex functions as a signaling receptor for the ligand receptor ICAM1, ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). Integrin ITGAL/ITGB2 that functions as a signaling receptor of ICAM2, ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 1676048).

See full target information ITGB2 pS745

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0331900 PubMed40939011

2025

C3AR1 may aggravate diabetic nephropathy by mediating oxidative stress via ITGB2 regulation in renal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alex Abura,Shan Gao

BMC biology 23:268 PubMed40859304

2025

Cranial base synostosis in mice caused by upregulation of Wnt following partial inhibition of Shh.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangping Chen,Chengyan Ren,Chuanqing Mao,Yongzhen Lai,Meng Lu,Yuanjing Jiang,Weihui Chen

Journal of nanobiotechnology 23:134 PubMed39987112

2025

Engineered neutrophil membrane-camouflaged nanocomplexes for targeted siRNA delivery against myocardial ischemia reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yaohui Jiang,Rongyan Jiang,Zequn Xia,Meng Guo,Yanan Fu,Xiaocheng Wang,Jun Xie

Frontiers in pharmacology 12:759027 PubMed35095486

2022

QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Ye,Quan Li,Chun-Shui Pan,Li Yan,Kai Sun,Xiao-Yi Wang,Shu-Qi Yao,Jing-Yu Fan,Jing-Yan Han

Virulence 12:1980-1996 PubMed34338598

2021

Complement receptor 3 mediates internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Xuelin Han,Xueting Su,Zhiqian Li,Yanxi Liu,Shuo Wang,Miao Zhu,Changjian Zhang,Fan Yang,Jingya Zhao,Xianping Li,Fangyan Chen,Li Han

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD18 (phospho S745) antibody [EP1288Y]

Immunoprecipitation - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

Western blot - Anti-CD18 (phospho S745) antibody [EP1288Y] (AB52920)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise