Rat Monoclonal CD19 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 26 publications. Immunogen corresponding to Cell preparation containing Cd19 protein.
Images
![Flow Cytometry - Anti-CD19 antibody [6D5] (AB25232), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-6d5-ab25232--flow-cytometry-img59063.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD19 antibody [6D5] (AB25232)")
Publications
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- Nature communications 14:80482023Leukemia-intrinsic determinants of CAR-T response revealed by iterative in vivo genome-wide CRISPR screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAzucena Ramos,Catherine E Koch,Yunpeng Liu-Lupo,Riley D Hellinger,Taeyoon Kyung,Keene L Abbott,Julia Fröse,Daniel Goulet,Khloe S Gordon,Keith P Eidell,Paul Leclerc,Charles A Whittaker,Rebecca C Larson,Audrey J Muscato,Kathleen B Yates,Juan Dubrot,John G Doench,Aviv Regev,Matthew G Vander Heiden,Marcela V Maus,Robert T Manguso,Michael E Birnbaum,Michael T HemannPubMed 38052854
- Frontiers in cardiovascular medicine 10:11521242023Injury to the tunica media initiates atherogenesis in the presence of hyperlipidemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanane Belhoul-Fakir,Jiansha Wu,Yen L Yeow,Gabrielle C Musk,Helen Kershaw,Evan Ingley,Bichen Sophie Zhao,Christopher M Reid,Christopher Lagat,Brian Evans,Peter L Thompson,Michael L Brown,Juliana Hamzah,Shirley JansenPubMed 37063951
- Molecular cancer research : MCR 21:155-1692022Gut-derived Endotoxin-TLR4 Signaling Drives MYC-Ig Translocation to Promote Lymphoproliferation through c-JUN and STAT3 Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed Rokan,Juan Carlos Hernandez,Rajeshwar Nitiyanandan,Zi-Ying Lin,Chia-Lin Chen,Tatsuya Machida,Meng Li,Jasleen Khanuja,Mo Li Chen,Stanley M Tahara,Imran Siddiqi,Keigo MachidaPubMed 36287175
- JCI insight 7:2022CXCL13-producing CD4+ T cells accumulate in the early phase of tertiary lymphoid structures in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasayo Ukita,Junzo Hamanishi,Hiroyuki Yoshitomi,Koji Yamanoi,Shiro Takamatsu,Akihiko Ueda,Haruka Suzuki,Yuko Hosoe,Yoko Furutake,Mana Taki,Kaoru Abiko,Ken Yamaguchi,Hidekatsu Nakai,Tsukasa Baba,Noriomi Matsumura,Akihiko Yoshizawa,Hideki Ueno,Masaki MandaiPubMed 35552285
- Brain : a journal of neurology 142:2000-20122019Behaviour and neuropathology in mice injected with human contactin-associated protein 2 antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Pia Giannoccaro,David A Menassa,Leslie Jacobson,Ester Coutinho,Gennaro Prota,Bethan Lang,M Isabel Leite,Vincenzo Cerundolo,Rocco Liguori,Angela VincentPubMed 31079141
- EBioMedicine 37:38-462018In situ administration of cytokine combinations induces tumor regression in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinyu Zhang et. al.PubMed 30297145
- Experimental and therapeutic medicine 16:4225-42312018Tanshinone IIA improves diabetes mellitus via the NF-κB-induced AMPK signal pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng-Yi Yuan et. al.PubMed 30344697
- FASEB journal : official publication of the Federa 32:3336-33452018Depletion of microglia augments the dopaminergic neurotoxicity of MPTP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxia Yang et. al.PubMed 29401614
- Journal of orthopaedic research : official publica 35:2425-24342017Diminished bone regeneration after debridement of posttraumatic osteomyelitis is accompanied by altered cytokine levels, elevated B cell activity, and increased osteoclast activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohannes M Wagner et. al.PubMed 28263017
- Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale 37:207-2132016An "ex vivo model" contributing to the diagnosis and evaluation of new drugs in cystic fibrosis.Applications:ICCReactive species:HumanA M Di Lullo,M Scorza,F Amato,M Comegna,V Raia,L Maiuri,G Ilardi,E Cantone,G Castaldo,M IengoPubMed 27897275
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
pH: 8.2
Constituents: 100% Borate buffered saline- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Cell preparation containing Cd19 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P25918
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Associated Products
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8 products for Alternative Product
Target data
Function
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:20101619, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:7542548, PubMed:7543183, PubMed:9317126). Required for normal differentiation of B-1 cells (PubMed:12387743, PubMed:7542548, PubMed:7543183). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:12387743, PubMed:7542548, PubMed:9317126). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:7542548, PubMed:7543183).
Alternative names
B-lymphocyte antigen CD19, Differentiation antigen CD19, Cd19
Recommended products
Rat Monoclonal CD19 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 26 publications. Immunogen corresponding to Cell preparation containing Cd19 protein.
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
pH: 8.2
Constituents: 100% Borate buffered saline- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Cell preparation containing Cd19 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P25918
- Clone number
6D5
- Purification technique
Affinity purification
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.
- Biological function summary
CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.
- Pathways
The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.
- Associated diseases and disorders
CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - Anti-CD19 antibody [6D5] (ab25232), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-6d5-ab25232--flow-cytometry-img59063.jpg "Flow Cytometry - Anti-CD19 antibody [6D5] (ab25232)")
Flow Cytometry - Anti-CD19 antibody [6D5] (ab25232)
Flow Cytometry analysis of BALB/c mouse splenocyte cells labeling CD19 with ab25232 at 1 μg/106 cells dilution, followed by Rat Anti-Mouse CD3ε-FITC then by Mouse Anti-Rat IgG2a-PE.
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