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Rabbit Polyclonal CD19 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD19.


Images

Flow Cytometry - Anti-CD19 antibody (AB227019), expandable thumbnail
  • Western blot - Anti-CD19 antibody (AB227019), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD19 antibody (AB227019), expandable thumbnail
  • Western blot - Anti-CD19 antibody (AB227019), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human CD19. The exact immunogen used to generate this antibody is proprietary information. Database link P15391

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Expected
Tested
Tested
Mouse
Tested
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/50.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126).

Alternative names

Recommended products

Rabbit Polyclonal CD19 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD19.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human CD19. The exact immunogen used to generate this antibody is proprietary information. Database link P15391
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

Associated diseases and disorders

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry - Anti-CD19 antibody (ab227019), expandable thumbnail

    Flow Cytometry - Anti-CD19 antibody (ab227019)

    Flow cytometric analysis of 4% paraformaldehyde-fixed mouse splenocytes labeling CD19 with ab227019 at 1/50 dilution (red) compared with an unlabeled sample as a control (black).

    Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

  • Western blot - Anti-CD19 antibody (ab227019), expandable thumbnail

    Western blot - Anti-CD19 antibody (ab227019)

    7.5% SDS-PAGE gel.

    All lanes: Western blot - Anti-CD19 antibody (ab227019) at 1/1000 dilution

    All lanes: Mouse leukocyte extract at 50 µg

    Predicted band size: 61 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD19 antibody (ab227019), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD19 antibody (ab227019)

    Raji (human Burkitt's lymphoma cell line) cells stained for CD19 (green) using ab227019 at 1/1000 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.

    Blue: Hoechst 33342 staining.

  • Western blot - Anti-CD19 antibody (ab227019), expandable thumbnail

    Western blot - Anti-CD19 antibody (ab227019)

    7.5 % SDS-PAGE gel.

    All lanes: Western blot - Anti-CD19 antibody (ab227019) at 1/1000 dilution

    All lanes: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Predicted band size: 61 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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