AB254170

Anti-CD19 antibody [BU12]

Recombinant
Lab Essentials
What is this?

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(5 Publications)

Mouse Recombinant Monoclonal CD19 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

1 Images

Flow Cytometry - Anti-CD19 antibody [BU12] (AB254170)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD19 antibody [BU12] (AB254170)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD19 with ab254170 at 1.07 μg/mL, Right compared with a Mouse monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with mouse IgG (Left) or ab254170 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor^® 647. Gated on viable cells.

Carrier free

Anti-CD19 antibody [BU12] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD19 antibody [BU12]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BU12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.07 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed : 29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed : 1373518, PubMed : 16672701, PubMed : 2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 1373518, PubMed : 2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126).

See full target information CD19

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:17416-17434 PubMed40352528

2025

Mercury Can Bond to α-Carbon of Curcumin Increasing Stability in Aqueous Medium and Demonstrated Selective Cytotoxicity against Acute Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Sougata Mondal,Upasana Das,Oyendrila Ghosh,Bidisha Maiti,Shuvam Halder,Uttam Pal,Kalyan Kusum Mukherjee,Supratim Ghosh

Toxins 8: PubMed27338475

2016

High in Vitro Anti-Tumor Efficacy of Dimeric Rituximab/Saporin-S6 Immunotoxin.

Applications

Unspecified application

Species

Unspecified reactive species

Massimo Bortolotti,Andrea Bolognesi,Maria Giulia Battelli,Letizia Polito

British journal of haematology 134:157-70 PubMed16771848

2006

The anti-CD20 antibody rituximab augments the immunospecific therapeutic effectiveness of an anti-CD19 immunotoxin directed against human B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

David J Flavell,Sarah L Warnes,Christine J Bryson,Sarah A Field,Armorel L Noss,Graham Packham,Sopsamorn U Flavell

The Journal of experimental medicine 182:1191-9 PubMed7595190

1995

Engaging CD19 or target of an antiproliferative antibody 1 on human B lymphocytes induces binding of B cells to the interfollicular stroma of human tonsils via integrin alpha 4/beta 1 and fibronectin.

Applications

Unspecified application

Species

Unspecified reactive species

S Behr,F Schriever

Journal of immunology (Baltimore, Md. : 1950) 148:2983-7 PubMed1374445

1992

CD19 regulation of human B cell responses. B cell proliferation and antibody secretion are inhibited or enhanced by ligation of the CD19 surface glycoprotein depending on the stimulating signal used.

Applications

Unspecified application

Species

Unspecified reactive species

R E Callard,K P Rigley,S H Smith,S Thurstan,J G Shields

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD19 antibody [BU12]

Flow Cytometry - Anti-CD19 antibody [BU12] (AB254170)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise