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AB1255

Anti-CD19 antibody [CB19]

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(5 Publications)

Mouse Monoclonal CD19 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing CD19 protein.

View Alternative Names

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

  • 519 FITC

    FITC Anti-CD19 antibody [CB19]

  • 660 APC

    APC Anti-CD19 antibody [CB19]

  • 578 PE

    PE Anti-CD19 antibody [CB19]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CB19

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD19 protein. The exact immunogen used to generate this antibody is proprietary information.

P15391

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p><p>Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.1% Sodium azide Constituents: 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.
Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed : 29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed : 1373518, PubMed : 16672701, PubMed : 2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 1373518, PubMed : 2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 16672701, PubMed : 9317126).
See full target information CD19
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 24:1499-1503 PubMed30275568

2018

Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Ruella,Jun Xu,David M Barrett,Joseph A Fraietta,Tyler J Reich,David E Ambrose,Michael Klichinsky,Olga Shestova,Prachi R Patel,Irina Kulikovskaya,Farzana Nazimuddin,Vijay G Bhoj,Elena J Orlando,Terry J Fry,Hans Bitter,Shannon L Maude,Bruce L Levine,Christopher L Nobles,Frederic D Bushman,Regina M Young,John Scholler,Saar I Gill,Carl H June,Stephan A Grupp,Simon F Lacey,J Joseph Melenhorst

Journal of immunology (Baltimore, Md. : 1950) 199:1003-1011 PubMed28667164

2017

Immune Cell Dynamics in Rhesus Macaques Infected with a Brazilian Strain of Zika Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo L V Silveira,Kenneth A Rogers,Sanjeev Gumber,Praveen Amancha,Peng Xiao,Shawna M Woollard,Siddappa N Byrareddy,Mauro Martins Teixeira,Francois Villinger

Xenotransplantation 22:221-30 PubMed25847130

2015

Pre-transplant antibody screening and anti-CD154 costimulation blockade promote long-term xenograft survival in a pig-to-primate kidney transplant model.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Higginbotham,Dave Mathews,Cynthia A Breeden,Mingqing Song,Alton Brad Farris,Christian P Larsen,Mandy L Ford,Andrew J Lutz,Matthew Tector,Kenneth A Newell,A Joseph Tector,Andrew B Adams

Journal of immunology (Baltimore, Md. : 1950) 149:2841-50 PubMed1383329

1992

The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules.

Applications

Unspecified application

Species

Unspecified reactive species

L E Bradbury,G S Kansas,S Levy,R L Evans,T F Tedder

Science (New York, N.Y.) 256:105-7 PubMed1373518

1992

CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

R H Carter,D T Fearon
View all publications
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