Anti-CD19 antibody [EPR28949-559] (ab320735)

Rabbit Recombinant Monoclonal CD19 antibody. Suitable for mIHC, IHC-P and reacts with Human samples.

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Images

Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (AB320735), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IHC-P	Flow Cyt
Human	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Target data

See full target information CD19

Function

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126).

Alternative names

CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12

Recommended products

Rabbit Recombinant Monoclonal CD19 antibody. Suitable for mIHC, IHC-P and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28949-559
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

Associated diseases and disorders

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (ab320735)
CD19 Multiplex immunohistochemistry staining of human liver tissue using rabbit Anti-CD19 antibody
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, ab320735 anti-CD19 used at 1:2000 (0.255 ug/ml) dilution and Anti-CD3 epsilon antibody [CAL57] ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.
Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™570) and anti-CD3 (magenta; Opal™690) on human liver.

Panel B: anti-MARCO staining macrophages in human liver.

Panel C: ant-CD19 staining B lymphocytes in human liver.

Panel D: ant-CD3 staining T lymphocytes in human liver.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, ab320735 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (ab320735)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human colon staining of PVRIG/CD112R with Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348 at a 1/100 (5.16 µg/ml) dilution, CD19 with ab320735 at 1/2000 (0.255 µg/ml) dilution and CD8A with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/1000 ( 0.375 µg/ml) dilution.
Panel A: merged staining of anti-PVRIG/CD112R (green; Opal™520), anti-CD8A (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on Human colon.
Panel B: anti-PVRIG/CD112R staining memory and effector CD8+ T lymphocytes in Human colon.
Panel C: anti-CD8A staining CD8+ T lymphocytes in Human colon.
Panel D: anti-CD19 staining B lymphocytes in Human colon.
Nuclear DNA was labelled with DAPI (shown in blue). ).
The section was incubated in three rounds of staining: in the order of Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348, Anti-CD8 alpha antibody [EPR22483-288] ab245118 and ab320735 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (ab320735)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human spleen staining of PVRIG/CD112R with Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348 at a 1/100 (5.16 µg/ml) dilution, CD19 with ab320735 at 1/2000 (0.255 µg/ml) dilution and CD8A with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/1000 ( 0.375 µg/ml) dilution.
Panel A: merged staining of anti-PVRIG/CD112R (green; Opal™520), anti-CD8A (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on human spleen.
Panel B: anti-PVRIG/CD112R staining memory and effector CD8+ T lymphocytes in human spleen.
Panel C: anti-CD8A staining CD8+ T lymphocytes in human spleen.
Panel D: anti-CD19 staining B lymphocytes in human spleen.
Nuclear DNA was labelled with DAPI (shown in blue). ).
The section was incubated in three rounds of staining: in the order of Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348, Anti-CD8 alpha antibody [EPR22483-288] ab245118 and ab320735 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (ab320735)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil staining of PVRIG/CD112R with Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348 at a 1/100 (5.16 µg/ml) dilution, CD19 with ab320735 at 1/2000 (0.255 µg/ml) dilution and CD8A with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/1000 ( 0.375 µg/ml) dilution.
Panel A: merged staining of anti-PVRIG/CD112R (green; Opal™520), anti-CD8A (magenta; Opal™570) and anti-CD19 (yellow; Opal™690) on human tonsil.
Panel B: anti-PVRIG/CD112R staining memory and effector CD8+ T lymphocytes in human tonsil.
Panel C: anti-CD8A staining CD8+ T lymphocytes in human tonsil.
Panel D: anti-CD19 staining B lymphocytes in human tonsil.
Nuclear DNA was labeled with DAPI (shown in blue). ).
The section was incubated in three rounds of staining: in the order of Anti-PVRIG/CD112R antibody [EPR29052-91] ab322348, Anti-CD8 alpha antibody [EPR22483-288] ab245118 and ab320735 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (ab320735)
Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human tonsil.
Panel B: anti-CD19 staining the B lymphocytes in human tonsil.
Panel C: anti-CD19 staining the B lymphocytes in human tonsil.
Panel D: anti-CD3 staining the T lymphocytes in human tonsil.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab320735 at 1/2000 dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (ab320735)
Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma.
Panel B: anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma.
Panel C: anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma.
Panel D: anti-CD3 staining the T lymphocytes in human Hodgkin's lymphoma.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab320735 at 1/2000 dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-CD19 antibody [EPR28949-559] (ab320735)
Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (green; Opal™570) and anti-CD3 (yellow; Opal™690) on human cerebrum.
Panel B: anti-CD19 showed no staining in human cerebrum.
Panel C: anti-CD19 showed no staining in human cerebrum.
Panel D: anti-CD3 showed no staining in human cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab320735 at 1/2000 dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR28949-559] (ab320735)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CD19 with ab320735 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control: No staining in human liver.
The section was incubated with ab320735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR28949-559] (ab320735)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling CD19 with ab320735 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control: No staining in human cerebrum (PMID: 32961131).
The section was incubated with ab320735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR28949-559] (ab320735)
Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD19 with ab320735 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human Hodgkin's lymphoma.
The section was incubated with ab320735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [EPR28949-559] (ab320735)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD19 with ab320735 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human tonsil.
The section was incubated with ab320735 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins