Rabbit Recombinant Monoclonal CD19 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (AB324080), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-epr29472-38-bsa-and-azide-free-capture-ab324080--sandwich-elisa-img446589.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (AB324080)")
![Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (AB324080), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-epr29472-38-bsa-and-azide-free-capture-ab324080--sandwich-elisa-img446590.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (AB324080)")
![Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (AB324080), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-epr29472-38-bsa-and-azide-free-capture-ab324080--sandwich-elisa-img446591.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (AB324080)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126).
Alternative names
CD19, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12
Rabbit Recombinant Monoclonal CD19 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29472-38
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab324080 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CD19. The recommended pair for sandwich ELISA is:
Capture: ab324080, Human CD19 Capture Antibody (unconjugated)
Detector: Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) ab324082, Human CD19 Detector Antibody (unconjugated)
The reference range value is 19.5-5000 pg/mL.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-epr29472-38-bsa-and-azide-free-capture-ab324080--sandwich-elisa-img446589.jpg "Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080)")
Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080)
Sandwich ELISA using Human CD19 immunogen protein standard curve with the capture antibody ab324080 at 2 ug/mL and detector antibody Anti-CD19 antibody [EPR29472-65] - BSA and Azide free (Detector) ab324082 at 0.5 ug/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)
![Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-epr29472-38-bsa-and-azide-free-capture-ab324080--sandwich-elisa-img446590.jpg "Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080)")
Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080)
Interpolated concentration of native CD19 was measured at different sample concentrations. Undiluted sample is as follows: Raji cell extract (0.25 mg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
![Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080), expandable thumbnail](https://content.abcam.com/products/images/cd19-antibody-epr29472-38-bsa-and-azide-free-capture-ab324080--sandwich-elisa-img446591.jpg "Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080)")
Sandwich ELISA - Anti-CD19 antibody [EPR29472-38] - BSA and Azide free (Capture) (ab324080)
Interpolated concentration of native CD19 was measured at different sample concentrations. Undiluted sample is as follows: Daudi cell extract (0.25 mg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com