JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB25177

Anti-CD19 antibody [MB19-1]

Be the first to review this product! Submit a review

|

(5 Publications)

Mouse Monoclonal CD19 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 5 publications.

View Alternative Names

CD19, B-lymphocyte antigen CD19, Differentiation antigen CD19, Cd19

1 Images
Flow Cytometry - Anti-CD19 antibody [MB19-1] (AB25177)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD19 antibody [MB19-1] (AB25177)

C57BL6 mouse splenocytes were incubated with Mouse IgA kappa monoclonal [S107] (ab37322) (left) or anti-CD19 [MB19-1] antibody (ab25177) (right) at 1ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Mouse IgA alpha chain (FITC) was used at 1/200 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD19 APC (10ug/ml).

Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes

  • 578 PE

    PE Anti-CD19 antibody [MB19-1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MB19-1

Isotype

IgA

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB274888

Alexa Fluor® 555 Anti-CD19 antibody [EPR5906]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-CD19 antibody [EPR5906] (AB274888)

  • 5
  • 1
Primary Antibodies

AB225717

Anti-CD19 antibody [SP110] - C-terminal

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP110] - C-terminal (AB225717)

  • 0
  • 0
Primary Antibodies

AB1168

PE Anti-CD19 antibody [CB19]

Lab Essentials

Flow Cytometry - PE Anti-CD19 antibody [CB19] (AB1168)

  • 5
  • 1
Primary Antibodies

AB134114

Anti-CD19 antibody [EPR5906]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-CD19 antibody [EPR5906] (AB134114)

  • 4
  • 5

Complementary Products

Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 79
Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

  • 5
  • 21
Primary Antibodies

AB217344

Anti-CD8 alpha antibody [EPR21769]

RabMAb|Recombinant

Flow Cytometry - Anti-CD8 alpha antibody [EPR21769] (AB217344)

  • 5
  • 6
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-iga-kappa-s107-isotype-control-ab37322'>ab37322</a> - Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purification notes
Diafiltration.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.
Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (By similarity). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed : 12387743, PubMed : 20101619, PubMed : 9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed : 7542548, PubMed : 7543183, PubMed : 9317126). Required for normal differentiation of B-1 cells (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed : 12387743, PubMed : 7542548, PubMed : 9317126). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed : 12387743, PubMed : 7542548, PubMed : 7543183).
See full target information Cd19
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of 24:6015-6027 PubMed30262507

2018

A Designer Cross-reactive DNA Immunotherapeutic Vaccine that Targets Multiple MAGE-A Family Members Simultaneously for Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth K Duperret,Shujing Liu,Megan Paik,Aspen Trautz,Regina Stoltz,Xiaoming Liu,Kan Ze,Alfredo Perales-Puchalt,Charles Reed,Jian Yan,Xiaowei Xu,David B Weiner

The Journal of experimental medicine 186:1923-31 PubMed9382890

1998

CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

M Inaoki,S Sato,B C Weintraub,C C Goodnow,T F Tedder

Proceedings of the National Academy of Sciences of the United States of America 94:13158-62 PubMed9371816

1997

CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling.

Applications

Unspecified application

Species

Unspecified reactive species

S Sato,P J Jansen,T F Tedder

Journal of immunology (Baltimore, Md. : 1950) 159:3278-87 PubMed9317126

1997

Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19.

Applications

Unspecified application

Species

Unspecified reactive species

S Sato,A S Miller,M C Howard,T F Tedder

Immunity 5:551-62 PubMed8986715

1996

CD22 is both a positive and negative regulator of B lymphocyte antigen receptor signal transduction: altered signaling in CD22-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

S Sato,A S Miller,M Inaoki,C B Bock,P J Jansen,M L Tang,T F Tedder
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com