Anti-CD19 antibody [SP291] - BSA and Azide free

25 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD19 with Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/100 dilution (1.50 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with Anti-CD19 antibody [SP291] - C-terminal ab227688 for 10 mins at room temperature. This image was generated using Anti-CD19 antibody [SP291] - C-terminal ab227688, the same clone, but with a different buffer formulation.

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  Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Intracellular Flow Cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/20 dilution (7.5μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

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  Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma cell line) cells, labeling CD19 with Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Formalin-fixed, paraffin-embedded human colon tissue stained for CD19 using Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Formalin-fixed, paraffin-embedded human spleen tissue stained for CD19 using Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Formalin-fixed, paraffin-embedded human thymus tissue stained for CD19 using Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Formalin-fixed, paraffin-embedded human B-Cell lymphoma tissue stained for CD19 using Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD19 using Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/100 dilution in immunohistochemical analysis.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD19 using Anti-CD19 antibody [SP291] - C-terminal ab227688 at 1/100 dilution in immunohistochemical analysis.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD19 antibody [SP291] - C-terminal ab227688).

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon cancer.

  Panel B: anti-CD208 staining dendritic cells in human colon cancer.

  Panel C: anti-CD3E staining T lymphocytes in human colon cancer.

  Panel D: anti-CD19 staining B lymphocytes of human colon cancer.

  The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon.

  Panel B: anti-CD208 staining dendritic cells in human colon.

  Panel C: anti-CD3E staining T lymphocytes in human colon.

  Panel D: anti-CD19 staining B lymphocytes of human colon.

  The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human tonsil.

  Panel B: anti-CD208 staining dendritic cells in human tonsil.

  Panel C: anti-CD3E staining T lymphocytes in human tonsil.

  Panel D: anti-CD19 staining B lymphocytes of human tonsil.

  The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining TCF7 with Anti-TCF7 antibody [EPR28579-32] ab315390 at a 1:2000 (0.26 ug/ml) dilution, Anti-CD3 epsilon antibody [CAL54] ab237707 anti-CD3E used at 1:500 (1.01 ug/ml) dilution and ab237772 anti-CD19 used at a 1:5000 (0.21 ug/ml) dilution.

  Panel A: merged staining of anti-TCF7 (magenta; Opal™690), anti-CD3E (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
  
  Panel B: anti-TCF7 staining T lymphocytes of human tonsil.
  
  Panel C: anti-CD3E staining T lymphocytes of human tonsil.
  
  Panel D: anti-CD19 staining B lymphocytes of human tonsil.

  The section was incubated in three rounds of staining: in the order of Anti-TCF7 antibody [EPR28579-32] ab315390, Anti-CD3 epsilon antibody [CAL54] ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at a 1:2000 (0.26 ug/ml) dilution, Anti-CD3 epsilon antibody [CAL54] ab237707 anti-CD3E used at 1:500 (1.01 ug/ml) dilution and ab237772 anti-CD19 used at a 1:5000 (0.21 ug/ml) dilution.

  Panel A: merged staining of anti-TCF7 (magenta; Opal™690), anti-CD3E (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
  
  Panel B: anti-TCF7 staining T lymphocytes of human tonsil.
  
  Panel C: anti-CD3E staining T lymphocytes of human tonsil.
  
  Panel D: anti-CD19 staining B lymphocytes of human tonsil.

  The section was incubated in three rounds of staining: in the order of Anti-TCF7 antibody [EPR28579-5] ab315392, Anti-CD3 epsilon antibody [CAL54] ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling CD8 alpha with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/500 dilution, CD4 with Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 at 1/500, and CD19 with ab237772 at 1/5000 dilution.
  
  Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
  Panel B: anti-CD8 alpha stained on cytotoxic T cells.
  Panel C: anti-CD4 stained on T helper cells.
  Panel D: anti-CD19 stained on B cells.
  
  Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of Anti-CD8 alpha antibody [EPR22483-288] ab245118 for 30 mins, then Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 and ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  DAPI was used as a nuclear counterstain.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling CD8 alpha with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/500 dilution, CD4 with Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 at 1/500, and CD19 with ab237772 at 1/5000 dilution.
  
  Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
  Panel B: anti-CD8 alpha stained on cytotoxic T cells.
  Panel C: anti-CD4 stained on T helper cells.
  Panel D: anti-CD19 stained on B cells.
  
  Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of Anti-CD8 alpha antibody [EPR22483-288] ab245118 for 30 mins, then Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 and ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  DAPI was used as a nuclear counterstain.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human tonsil.
  Panel B: anti-CD19 staining the B lymphocytes in human tonsil.
  Panel C: anti-CD19 staining the B lymphocytes in human tonsil.
  Panel D: anti-CD3 staining the T lymphocytes in human tonsil.
  Nuclear DNA was labeled with DAPI (shown in blue).
  The section was incubated in three rounds of staining: in the order of Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 dilution, ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma.
  Panel B: anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma.
  Panel C: anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma.
  Panel D: anti-CD3 staining the T lymphocytes in human Hodgkin's lymphoma.
  Nuclear DNA was labeled with DAPI (shown in blue).
  The section was incubated in three rounds of staining: in the order of Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 dilution, ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (green; Opal™570) and anti-CD3 (yellow; Opal™690) on human cerebrum.
  Panel B: anti-CD19 showed no staining in human cerebrum.
  Panel C: anti-CD19 showed no staining in human cerebrum.
  Panel D: anti-CD3 showed no staining in human cerebrum.
  Nuclear DNA was labeled with DAPI (shown in blue).
  The section was incubated in three rounds of staining: in the order of Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 dilution, ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  This data was developed using Anti-CD3 epsilon antibody [SP7] ab16669, the same antibody clone in a different buffer formulation.
  
  Panel A: merged staining of anti-CD68 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, and counterstaining was with DAPI.
  
  Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
  Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
  Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution
  
  The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  This image was generated using Anti-CD19 antibody [SP291] - C-terminal ab227688, the same clone, but with a different buffer formulation.
  Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, and couterstaining was with DAPI.
  Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
  Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
  Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution
  The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  This image was generated using Anti-CD19 antibody [SP291] - C-terminal ab227688, the same clone, but with a different buffer formulation.
  
  Panel A: merged staining of anti-CD31 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, nuclear counterstain was DAPI.
  Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
  Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
  Panel D: anti-CD31 stained on endothelial cells and immune cell subsets with Anti-CD31 antibody [EPR3094] - BSA and Azide free ab207090 at 1/500 dilution
  The section was incubated in three rounds of staining: in the order of Anti-CD31 antibody [EPR3094] - BSA and Azide free ab207090 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Panel A: merged staining of anti-CD31 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, nuclear counterstain was DAPI.
  Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
  Panel C: anti-CD19 stained on B cells with ab237772 at 1/5000 dilution
  Panel D: anti-CD31 stained on endothelial cells with Anti-CD31 antibody [EPR3094] - BSA and Azide free ab207090 at 1/500 dilution
  
  The section was incubated in three rounds of staining: in the order of Anti-CD31 antibody [EPR3094] - BSA and Azide free ab207090 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins"

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at a 1:50 (10.56 ug/ml) dilution, ab237772 anti-CD19 used at 1:5000 (0.21 ug/ml) dilution and Anti-CD3 epsilon antibody [CAL54] ab237707 anti-CD3E used at a 1:500 (1.01 ug/ml) dilution.

  Panel A: merged staining of anti-CD21 (green; Opal™690), anti-CD3E (grey; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
  
  Panel B: anti-CD21 staining mature B lymphocytes and T-lymphocytes of human tonsil.
  
  Panel C: anti-CD19 staining B lymphocytes of human tonsil.
  
  Panel D: anti-CD3E staining T lymphocytes of human tonsil.

  The section was incubated in three rounds of staining: in the order of Anti-CD21 antibody [EPR27369-9] ab315160, ab237772, and Anti-CD3 epsilon antibody [CAL54] ab237707 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Nuclear counter stain with DAPI.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - BSA and Azide free (ab237772)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human liver cancer.

  Panel B: anti-CD208 staining dendritic cells in human liver cancer.

  Panel C: anti-CD3E staining T lymphocytes in human liver cancer.

  Panel D: anti-CD19 staining B lymphocytes of human liver cancer.

  The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.