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Rabbit Recombinant Monoclonal CD19 antibody. C-terminal. Suitable for mIHC, Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (AB227688), expandable thumbnail
  • Western blot - Anti-CD19 antibody [SP291] - C-terminal (AB227688), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (AB227688), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (AB227688), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCFlow Cyt (Intra)WBIHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/20 - 1/400

Notes

-

Tested
Tested

Species

Human

Dilution info

1/400

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Associated Products

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Target data

Function

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD19 antibody. C-terminal. Suitable for mIHC, Flow Cyt (Intra), WB, IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP291

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD19 is a transmembrane protein also known as B4 or B-lymphocyte antigen CD19 with an approximate molecular weight of 95 kDa. It is expressed on the surface of B cells throughout their development from pro-B cells to mature B cells. CD19 functions mechanically as a coreceptor enhancing the sensitivity of B cell receptor (BCR) signaling. This protein plays a significant role in promoting the activation and proliferation of B cells by lowering the threshold for antigen receptor engagement.

Biological function summary

CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.

Pathways

The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.

Associated diseases and disorders

CD19 is connected to B cell malignancies and autoimmune diseases. B cell acute lymphoblastic leukemia (ALL) often shows aberrant CD19 expression making it a valuable target for therapeutic interventions like anti-CD19 monoclonal antibodies. Additionally overexpression or mutations of CD19 may contribute to autoimmune diseases by disrupting normal B cell regulation. CD19's connection with proteins like CD22 and CD20 in these pathological contexts highlights its relevance in developing targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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22 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD19 with ab227688 at 1/100 dilution (1.50 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Membranous staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227688 for 10 mins at room temperature.

  • Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Flow cytometry analysis of Raji (human Burkitt's lymphoma) labeling CD19 with purified ab227688 at 1/20 dilution (7.5 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Western blot - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Western blot - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    All lanes: Western blot - Anti-CD19 antibody [SP291] - C-terminal (ab227688) at 1/400 dilution

    All lanes: Ramos (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Flow Cytometry analysis of Ramos (human Burkitt's lymphoma cell line) cells, labeling CD19 with ab227688 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human B-Cell lymphoma tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD19 using ab227688 at 1/100 dilution in immunohistochemical analysis.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human tonsil.

    Panel B: anti-CD208 staining dendritic cells in human tonsil.

    Panel C: anti-CD3E staining T lymphocytes in human tonsil.

    Panel D: anti-CD19 staining B lymphocytes of human tonsil.

    The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.


    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human spleen tissue labeling CD8 alpha with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/500 dilution, CD4 with Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 at 1/500, and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution.

    Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human spleen.
    Panel B: anti-CD8 alpha stained on cytotoxic T cells.
    Panel C: anti-CD4 stained on T helper cells.
    Panel D: anti-CD19 stained on B cells.

    Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of Anti-CD8 alpha antibody [EPR22483-288] ab245118 for 30 mins, then Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    DAPI was used as a nuclear counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.


    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human tonsil tissue labeling CD8 alpha with Anti-CD8 alpha antibody [EPR22483-288] ab245118 at 1/500 dilution, CD4 with Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 at 1/500, and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution.

    Panel A: merged staining of anti-CD8 alpha (magenta; Opal™690), anti-CD4 (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
    Panel B: anti-CD8 alpha stained on cytotoxic T cells.
    Panel C: anti-CD4 stained on T helper cells.
    Panel D: anti-CD19 stained on B cells.

    Sections  were treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins before antibody incubation. The section was incubated in three rounds of staining: in the order of Anti-CD8 alpha antibody [EPR22483-288] ab245118 for 30 mins, then Anti-CD4 antibody [SP35] - BSA and Azide free ab238798 and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    DAPI was used as a nuclear counterstain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.


    Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human tonsil.
    Panel B: anti-CD19 staining the B lymphocytes in human tonsil.
    Panel C: anti-CD19 staining the B lymphocytes in human tonsil.
    Panel D: anti-CD3 staining the T lymphocytes in human tonsil.
    Nuclear DNA was labeled with DAPI (shown in blue).
    The section was incubated in three rounds of staining: in the order of Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.


    Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma.
    Panel B: anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma.
    Panel C: anti-CD19 staining the B lymphocytes in human Hodgkin's lymphoma.
    Panel D: anti-CD3 staining the T lymphocytes in human Hodgkin's lymphoma.
    Nuclear DNA was labeled with DAPI (shown in blue).
    The section was incubated in three rounds of staining: in the order of Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.


    Panel A: merged staining of anti-CD19 (green; Opal™520), anti-CD19 (green; Opal™570) and anti-CD3 (yellow; Opal™690) on human cerebrum.
    Panel B: anti-CD19 showed no staining in human cerebrum.
    Panel C: anti-CD19 showed no staining in human cerebrum.
    Panel D: anti-CD3 showed no staining in human cerebrum.
    Nuclear DNA was labeled with DAPI (shown in blue).
    The section was incubated in three rounds of staining: in the order of Anti-CD19 antibody [EPR28949-559] ab320735 at 1/2000 dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution and Anti-CD3 epsilon antibody [SP7] ab16669 at 1/150 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Panel A: merged staining of anti-CD68 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, and counterstaining was with DAPI.

    Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
    Panel C: anti-CD19 stained on B cells with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution
    Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution

    The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Panel A: merged staining of anti-CD68 (magenta; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human spleen. Secondary antibody was Opal Polymer HRP Ms + Rb, and couterstaining was with DAPI.
    Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
    Panel C: anti-CD19 stained on B cells with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution
    Panel D: anti-CD68 stained on macrophages with Anti-CD68 antibody [EPR20545] ab213363 1/500 dilution
    The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR20545] ab213363 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    Panel A: merged staining of anti-CD31 (gray; Opal™690), anti-CD3 epsilon (green; Opal™520) and anti-CD19 (red; Opal™570) on Formalin/PFA-fixed paraffin-embedded sections of human tonsil. Secondary antibody was Opal Polymer HRP Ms + Rb, nuclear counterstain was DAPI.
    Panel B: anti-CD3 epsilon stained on T cells with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/500 dilution
    Panel C: anti-CD19 stained on B cells with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/5000 dilution
    Panel D: anti-CD31 stained on endothelial cells and immune cell subsets with Anti-CD31 antibody [EPR3094] - BSA and Azide free ab207090 at 1/500 dilution

    The section was incubated in three rounds of staining: in the order of Anti-CD31 antibody [EPR3094] - BSA and Azide free ab207090 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins, then Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 10 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human liver cancer.

    Panel B: anti-CD208 staining dendritic cells in human liver cancer.

    Panel C: anti-CD3E staining T lymphocytes in human liver cancer.

    Panel D: anti-CD19 staining B lymphocytes of human liver cancer.

    The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon cancer.

    Panel B: anti-CD208 staining dendritic cells in human colon cancer.

    Panel C: anti-CD3E staining T lymphocytes in human colon cancer.

    Panel D: anti-CD19 staining B lymphocytes of human colon cancer.

    The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD19 antibody [SP291] - C-terminal (ab227688)

    This data was developed using Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with Anti-CD3 epsilon antibody [CAL54] ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon.

    Panel B: anti-CD208 staining dendritic cells in human colon.

    Panel C: anti-CD3E staining T lymphocytes in human colon.

    Panel D: anti-CD19 staining B lymphocytes of human colon.

    The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, Anti-CD3 epsilon antibody [CAL54] ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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