Anti-CD1a antibody [EP3091] - BSA and Azide free

• WB

Lab

Western blot - Anti-CD1a antibody [EP3091] - BSA and Azide free (AB284760)

This data was developed using ab76531, the same antibody clone in a different buffer formulation.

False colour image of Western blot : Anti-CD1a antibody [EP3091] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76531 was shown to bind specifically to CD1a.

A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line ab274926 (knockout cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD1a antibody [EP3091] (<a href='/en-us/products/primary-antibodies/cd1a-antibody-ep3091-ab76531'>ab76531</a>) at 1/1000 dilution

Lane 1:

Wild-type Jurkat cell lysate at 20 µg

Lane 2:

CD1A knockout Jurkat cell lysate at 20 µg

Lane 2:

Western blot - Human CD1A knockout Jurkat cell line (<a href='/en-us/products/cell-lines/human-cd1a-knockout-jurkat-cell-line-ab274926'>ab274926</a>)

Lane 3:

MOLT-4 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat anti-Mouse IgG H&L preabsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 45-50 kDa

false

• WB

Unknown

Western blot - Anti-CD1a antibody [EP3091] - BSA and Azide free (AB284760)

This data was developed using ab76531, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD1a antibody [EP3091] (<a href='/en-us/products/primary-antibodies/cd1a-antibody-ep3091-ab76531'>ab76531</a>) at 1/5000 dilution

Lane 1:

MOLT4 lysate at 10 µg

Lane 2:

K562 lysate at 10 µg

Lane 3:

Fetal thymus lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

Observed band size: 47 kDa

false