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Rabbit Recombinant Monoclonal CD1A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-CD1a antibody [EP3622] - BSA and Azide free (AB217214), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] - BSA and Azide free (AB217214), expandable thumbnail
  • Western blot - Anti-CD1a antibody [EP3622] - BSA and Azide free (AB217214), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PWB
Human
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

2 products for Alternative Version

Target data

Function

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Alternative names

CD1a, T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

Recommended products

Rabbit Recombinant Monoclonal CD1A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP3622
Purification technique
Affinity purification Protein A
Dissociation constant
1.3 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab217214 is the carrier-free version of Anti-CD1a antibody [EP3622] ab108309.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD1a also known as T6 antigen or CD1 is a protein with an estimated molecular mass of 37 kDa. It is expressed mainly on the surface of Langerhans cells in the skin and dendritic cells in the thymus. CD1a belongs to the CD1 family which presents lipid antigens to T cells. The protein is an important marker in immunophenotyping of these dendritic cells making it useful in flow cytometry applications.

Biological function summary

CD1a participates in the immune system by presenting lipid and glycolipid antigens to T lymphocytes including natural killer T (NKT) cells. It forms a complex with β2-microglobulin which is essential for its proper function and antigen presentation. CD1a expression helps differentiate between types of dendritic cells with CD1a-positive cells often engaging in specific responses to microbial lipid antigens.

Pathways

CD1a plays a significant role in the antigen presentation pathway particularly in lipid antigen presentation to T cells. This is important for initiating immune responses. It interacts with related proteins such as CD1b and CD1c which also present lipid antigens. CD1a has implications in innate and adaptive immunity and can influence pathways involved in microbial recognition and elimination.

Associated diseases and disorders

CD1a is associated with certain skin conditions like Langerhans Cell Histiocytosis and psoriasis. It is involved in the pathogenesis of these diseases by affecting immune responses in the skin. In Langerhans Cell Histiocytosis CD1a-positive cells accumulate abnormally. Additionally CD1a is often studied alongside related proteins like CD207 (Langerin) to understand these conditions better.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-CD1a antibody [EP3622] - BSA and Azide free (ab217214), expandable thumbnail

    Western blot - Anti-CD1a antibody [EP3622] - BSA and Azide free (ab217214)

    False colour image of Western blot: Anti-CD1a antibody [EP3622] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD1a antibody [EP3622] ab108309 was shown to bind specifically to CD1a. A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line Human CD1A knockout Jurkat cell line ab274926 (knockout cell lysate Human CD1A knockout Jurkat cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-CD1a antibody [EP3622] (Anti-CD1a antibody [EP3622] ab108309) at 1/1000 dilution

    Lane 1: Wild-type Jurkat cell lysate at 20 µg

    Lane 2: CD1A knockout Jurkat cell lysate at 20 µg

    Lane 2: Western blot - Human CD1A knockout Jurkat cell line (Human CD1A knockout Jurkat cell line ab274926)

    Lane 3: MOLT-4 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 37 kDa

    Observed band size: 45-50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] - BSA and Azide free (ab217214), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] - BSA and Azide free (ab217214)

    This data was developed using Anti-CD1a antibody [EP3622] ab108309, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thymoma tissue sections labeling CD1a with purified Anti-CD1a antibody [EP3622] ab108309 at 1/1000 dilution (0.14 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-CD1a antibody [EP3622] - BSA and Azide free (ab217214), expandable thumbnail

    Western blot - Anti-CD1a antibody [EP3622] - BSA and Azide free (ab217214)

    This data was developed using Anti-CD1a antibody [EP3622] ab108309, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18178838).

    All lanes: Western blot - Anti-CD1a antibody [EP3622] (Anti-CD1a antibody [EP3622] ab108309) at 1/1000 dilution

    All lanes: Human thymus lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 37 kDa

    Observed band size: 49 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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