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Knockout Tested Rabbit Recombinant Monoclonal CD1A antibody. Carrier free. Suitable for Flow Cyt, IHC-P, WB, IP, mIHC and reacts with Human samples.

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Images

Western blot - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (AB313876), expandable thumbnail
  • Flow Cytometry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (AB313876), expandable thumbnail
  • Flow Cytometry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (AB313876), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (AB313876), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (AB313876), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PWBIPmIHCICC/IF
Human
Tested
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Alternative names

T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CD1A antibody. Carrier free. Suitable for Flow Cyt, IHC-P, WB, IP, mIHC and reacts with Human samples.

Alternative names

T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, hTa1 thymocyte antigen, CD1A

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR26526-62

Purification technique

Affinity purification Protein A

Specificity

This antibody does not cross-react with human CD1B.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab313876 is the carrier-free version of Anti-CD1a antibody [EPR26526-62] ab313875.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD1a also known as T6 antigen or CD1 is a protein with an estimated molecular mass of 37 kDa. It is expressed mainly on the surface of Langerhans cells in the skin and dendritic cells in the thymus. CD1a belongs to the CD1 family which presents lipid antigens to T cells. The protein is an important marker in immunophenotyping of these dendritic cells making it useful in flow cytometry applications.

Biological function summary

CD1a participates in the immune system by presenting lipid and glycolipid antigens to T lymphocytes including natural killer T (NKT) cells. It forms a complex with β2-microglobulin which is essential for its proper function and antigen presentation. CD1a expression helps differentiate between types of dendritic cells with CD1a-positive cells often engaging in specific responses to microbial lipid antigens.

Pathways

CD1a plays a significant role in the antigen presentation pathway particularly in lipid antigen presentation to T cells. This is important for initiating immune responses. It interacts with related proteins such as CD1b and CD1c which also present lipid antigens. CD1a has implications in innate and adaptive immunity and can influence pathways involved in microbial recognition and elimination.

Associated diseases and disorders

CD1a is associated with certain skin conditions like Langerhans Cell Histiocytosis and psoriasis. It is involved in the pathogenesis of these diseases by affecting immune responses in the skin. In Langerhans Cell Histiocytosis CD1a-positive cells accumulate abnormally. Additionally CD1a is often studied alongside related proteins like CD207 (Langerin) to understand these conditions better.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Western blot - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: HeLa.
    In Western blot, Anti-CD1a antibody [EPR26526-62] ab313875 was shown to bind specifically to CD1A. Target of interest was observed at 45-50 kDa in wild-type Jurkat cell lysates (lane 1) with no signal observed at this size in CD1A knockout cell line (lane 2) (lane 2, knockout cell line Human CD1A knockout Jurkat cell line ab274926).
    Samples are non-boiled as boiling may cause protein aggregation.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CD1a antibody [EPR26526-62] (Anti-CD1a antibody [EPR26526-62] ab313875) at 1/1000 dilution

    Lane 1: Wild-type Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 60 µg

    Lane 2: CD1A knockout Jurkat whole cell lysate at 60 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 60 µg

    Lane 4: MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate at 60 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 45-50 kDa

    Exposure time: 8s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: HeLa.
    In Western blot, Anti-CD1a antibody [EPR26526-62] ab313875 was shown to bind specifically to CD1A. Target of interest was observed at 45-50 kDa in wild-type Jurkat cell lysates (lane 1) with no signal observed at this size in CD1A knockout cell line (lane 2) (lane 2, knockout cell line Human CD1A knockout Jurkat cell line ab274926).
    Samples are non-boiled as boiling may cause protein aggregation.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  • Flow Cytometry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Flow Cytometry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa (human cervical adenocarcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CD1a with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: HeLa. Gated on viable cells.

  • Flow Cytometry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Flow Cytometry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.Flow cytometric analysis of CD1A KO Jurkat (CD1A knockout human T cell leukemia T lymphocyte from peripheral blood, Left) / Parental Jurkat (Right) cells labelling CD1a with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Gated on viable cells.

  • Multiplex immunohistochemistry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.

    Fluorescence multiplex immunohistochemical analysis of the human skin (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-CD1A (green; Opal™520) and anti-CD207 (red; Opal™570) on human skin.
    Panel B: anti-CD1A stained on Langerhans cells subsets.
    Panel C: anti-CD207 stained on Langerhans cells subsets.

    The section was incubated in two rounds of staining: in the order of Anti-Langerin antibody [EPR24685-12] ab283686 and Anti-CD1a antibody [EPR26526-62] ab313875 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling CD1a with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human cerebrum (PMID: 27666391). The section was incubated with Anti-CD1a antibody [EPR26526-62] ab313875 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling CD1a with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human thymoma (PMID: 35547871). The section was incubated with Anti-CD1a antibody [EPR26526-62] ab313875 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling CD1a with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on cortical thymocytes of human thymus (PMID: 28400115; PMID: 21565561). (A) Low‑powered (magnification, x100) and (B) high‑powered (magnification, x400) microscopic images. The section was incubated with Anti-CD1a antibody [EPR26526-62] ab313875 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD1a with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/10000 (0.052 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on epidermal Langerhans cells of human skin (PMID: 20514853). The section was incubated with Anti-CD1a antibody [EPR26526-62] ab313875 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunoprecipitation - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876), expandable thumbnail

    Immunoprecipitation - Anti-CD1a antibody [EPR26526-62] - BSA and Azide free (ab313876)

    This data was developed using Anti-CD1a antibody [EPR26526-62] ab313875, the same antibody clone in a different buffer formulation.
    CD1a was immunoprecipitated from 0.35 mg Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD1a antibody [EPR26526-62] ab313875 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
    Lane 2: Anti-CD1a antibody [EPR26526-62] ab313875 IP in Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD1a antibody [EPR26526-62] ab313875 in Jurkat whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CD1a antibody [EPR26526-62] (Anti-CD1a antibody [EPR26526-62] ab313875) at 1/30 dilution

    All lanes: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

    CD1a was immunoprecipitated from 0.35 mg Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate with Anti-CD1a antibody [EPR26526-62] ab313875 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD1a antibody [EPR26526-62] ab313875 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
    Lane 2: Anti-CD1a antibody [EPR26526-62] ab313875 IP in Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD1a antibody [EPR26526-62] ab313875 in Jurkat whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

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Product protocols

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