AB173576

Anti-CD1b antibody [EP7251]

RabMAb
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(3 Publications)

Rabbit Monoclonal CD1B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD1b, T-cell surface glycoprotein CD1b, CD1B

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

ab173576 showing -ve staining in Human normal pancreas tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

ab173576 showing -ve staining in Human endometrial adenocarcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

ab173576 showing -ve staining in Human skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] (AB173576)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] (AB173576)

Immunofluorescent staining of Jurkat cells labeling CD1b with ab173576 at 1/50 dilution

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1b with ab173576 at 1/100 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

ab173576 showing +ve staining in Human normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

ab173576 showing +ve staining in Human normal thymus tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-CD1b antibody [EP7251] (AB173576)

All lanes:

Western blot - Anti-CD1b antibody [EP7251] (ab173576) at 1/1000 dilution

Lane 1:

Human fetal thymus lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Predicted band size: 37 kDa

false

Carrier free

Anti-CD1b antibody [EP7251] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7251

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1b also known as CD1B is a member of the CD1 family of glycoproteins with a molecular mass around 37 to 40 kilodaltons. It is expressed on the surface of antigen-presenting cells such as dendritic cells and some monocytes. CD1b plays a mechanical role in binding lipid-based antigens. This binding facilitates the presentation of these antigens to T cells. Its presence on the cell surface is essential for this antigen presentation function.

Biological function summary

CD1b functions in the immune system as part of the antigen presentation complex. It specifically presents lipid antigens derived from microbial sources to T cells helping create an immune response. The protein associates with beta-2 microglobulin which stabilizes its structure on the cell surface. CD1b can effectively capture and present mycobacterial lipid antigens which are important for the immune response against certain bacterial infections.

Pathways

CD1b actively contributes to immunity by participating in the lipid antigen presentation pathway. This pathway involves the trafficking of endogenous and exogenous lipids aided by proteins like saposins. CD1b interacts with other CD1 family proteins such as CD1a and CD1c which are involved in similar pathways for presenting different types of lipid antigens to T cells therefore diversifying the immune response.

CD1b is implicated in tuberculosis as Mycobacterium tuberculosis interacts with this protein to modulate the host's immune defenses. Defective CD1b function can influence the body's response to this pathogen potentially leading to a chronic infection state. It also relates to autoimmune diseases such as multiple sclerosis where dysregulated lipid antigen presentation might influence disease progression. In these conditions interactions with proteins like HLA-DR can modify the immune landscape and disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

See full target information CD1B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:186 PubMed37344704

2023

Omentin-1 drives cardiomyocyte cell cycle arrest and metabolic maturation by interacting with BMP7.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun Yang,Shen Song,Jiacheng Li,Yandong Li,Jie Feng,Quan Sun,Xueting Qiu,Ziwei Chen,Xue Bai,Xinchang Liu,Hong Lian,Lihui Liu,Yongping Bai,Guogang Zhang,Yu Nie

International journal of general medicine 15:3809-3826 PubMed35418778

2022

CD1B is a Potential Prognostic Biomarker Associated with Tumor Mutation Burden and Promotes Antitumor Immunity in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou Li,Yanqi Feng,Piao Li,Shennan Wang,Xinyue Liu,Shu Xia

Frontiers in genetics 12:619821 PubMed34122500

2021

Comprehensive Profiling Reveals Distinct Microenvironment and Metabolism Characterization of Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Li,Chen Tian,Yangyang Liu,Jinyan Liang,Yulan Zeng,Qifan Yang,Yuting Liu,Di Wu,Jingjing Wu,Juanjuan Wang,Kai Zhang,Feifei Gu,Yue Hu,Li Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD1b antibody [EP7251]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] (AB173576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] (AB173576)

Western blot - Anti-CD1b antibody [EP7251] (AB173576)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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