Rabbit Monoclonal CD1B antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25256.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78354.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135233.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107841.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132005.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Alternative names
CD1b, T-cell surface glycoprotein CD1b, CD1B
Recommended products
Rabbit Monoclonal CD1B antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP7251
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab284822 is the carrier-free version of Anti-CD1b antibody [EP7251] ab173576
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD1b also known as CD1B is a member of the CD1 family of glycoproteins with a molecular mass around 37 to 40 kilodaltons. It is expressed on the surface of antigen-presenting cells such as dendritic cells and some monocytes. CD1b plays a mechanical role in binding lipid-based antigens. This binding facilitates the presentation of these antigens to T cells. Its presence on the cell surface is essential for this antigen presentation function.
- Biological function summary
CD1b functions in the immune system as part of the antigen presentation complex. It specifically presents lipid antigens derived from microbial sources to T cells helping create an immune response. The protein associates with beta-2 microglobulin which stabilizes its structure on the cell surface. CD1b can effectively capture and present mycobacterial lipid antigens which are important for the immune response against certain bacterial infections.
- Pathways
CD1b actively contributes to immunity by participating in the lipid antigen presentation pathway. This pathway involves the trafficking of endogenous and exogenous lipids aided by proteins like saposins. CD1b interacts with other CD1 family proteins such as CD1a and CD1c which are involved in similar pathways for presenting different types of lipid antigens to T cells therefore diversifying the immune response.
- Associated diseases and disorders
CD1b is implicated in tuberculosis as Mycobacterium tuberculosis interacts with this protein to modulate the host's immune defenses. Defective CD1b function can influence the body's response to this pathogen potentially leading to a chronic infection state. It also relates to autoimmune diseases such as multiple sclerosis where dysregulated lipid antigen presentation might influence disease progression. In these conditions interactions with proteins like HLA-DR can modify the immune landscape and disease outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25256.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1b with Anti-CD1b antibody [EP7251] ab173576 at 1/100 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78354.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.Anti-CD1b antibody [EP7251] ab173576 showing +ve staining in Human normal thymus tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135233.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.Anti-CD1b antibody [EP7251] ab173576 showing +ve staining in Human normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107841.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.Anti-CD1b antibody [EP7251] ab173576 showing -ve staining in Human normal pancreas tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132005.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.Anti-CD1b antibody [EP7251] ab173576 showing -ve staining in Human skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88480.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.Anti-CD1b antibody [EP7251] ab173576 showing -ve staining in Human endometrial adenocarcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunocytochemistry-immunofluorescence-img23123.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.Immunofluorescent staining of Jurkat cells labeling CD1b with Anti-CD1b antibody [EP7251] ab173576 at 1/50 dilution
![Western blot - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--western-blot-img147135.jpg "Western blot - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
Western blot - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)
This data was developed using Anti-CD1b antibody [EP7251] ab173576, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CD1b antibody [EP7251] (Anti-CD1b antibody [EP7251] ab173576) at 1/1000 dilution
Lane 1: Human fetal thymus lysate at 10 µg
Lane 2: Jurkat lysate at 10 µg
Predicted band size: 37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88480.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--immunocytochemistry-immunofluorescence-img23123.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
![Western blot - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822), expandable thumbnail](https://content.abcam.com/products/images/cd1b-antibody-ep7251-bsa-and-azide-free-ab284822--western-blot-img147135.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD1b antibody [EP7251] - BSA and Azide free (ab284822)")
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