AB284822

Anti-CD1b antibody [EP7251] - BSA and Azide free

RabMAb
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Rabbit Monoclonal CD1B antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.

View Alternative Names

CD1b, T-cell surface glycoprotein CD1b, CD1B

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.ab173576 showing -ve staining in Human normal pancreas tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.ab173576 showing -ve staining in Human endometrial adenocarcinoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.ab173576 showing -ve staining in Human skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.Immunofluorescent staining of Jurkat cells labeling CD1b with ab173576 at 1/50 dilution

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1b with ab173576 at 1/100 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.ab173576 showing +ve staining in Human normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.ab173576 showing +ve staining in Human normal thymus tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

This data was developed using ab173576, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD1b antibody [EP7251] (<a href='/en-us/products/primary-antibodies/cd1b-antibody-ep7251-ab173576'>ab173576</a>) at 1/1000 dilution

Lane 1:

Human fetal thymus lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Predicted band size: 37 kDa

false

Unconjugated

Anti-CD1b antibody [EP7251]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP7251

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab284822 is the carrier-free version of ab173576

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1b also known as CD1B is a member of the CD1 family of glycoproteins with a molecular mass around 37 to 40 kilodaltons. It is expressed on the surface of antigen-presenting cells such as dendritic cells and some monocytes. CD1b plays a mechanical role in binding lipid-based antigens. This binding facilitates the presentation of these antigens to T cells. Its presence on the cell surface is essential for this antigen presentation function.

Biological function summary

CD1b functions in the immune system as part of the antigen presentation complex. It specifically presents lipid antigens derived from microbial sources to T cells helping create an immune response. The protein associates with beta-2 microglobulin which stabilizes its structure on the cell surface. CD1b can effectively capture and present mycobacterial lipid antigens which are important for the immune response against certain bacterial infections.

Pathways

CD1b actively contributes to immunity by participating in the lipid antigen presentation pathway. This pathway involves the trafficking of endogenous and exogenous lipids aided by proteins like saposins. CD1b interacts with other CD1 family proteins such as CD1a and CD1c which are involved in similar pathways for presenting different types of lipid antigens to T cells therefore diversifying the immune response.

CD1b is implicated in tuberculosis as Mycobacterium tuberculosis interacts with this protein to modulate the host's immune defenses. Defective CD1b function can influence the body's response to this pathogen potentially leading to a chronic infection state. It also relates to autoimmune diseases such as multiple sclerosis where dysregulated lipid antigen presentation might influence disease progression. In these conditions interactions with proteins like HLA-DR can modify the immune landscape and disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

See full target information CD1B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD1b antibody [EP7251] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

Immunocytochemistry/ Immunofluorescence - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

Western blot - Anti-CD1b antibody [EP7251] - BSA and Azide free (AB284822)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise