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AB252262

Anti-CD1b antibody [WM25] - BSA and Azide free

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Mouse Recombinant Monoclonal CD1B antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD1b, T-cell surface glycoprotein CD1b, CD1B

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Flow Cytometry - Anti-CD1b antibody [WM25] - BSA and Azide free (AB252262)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD1b antibody [WM25] - BSA and Azide free (AB252262)

This data was developed using ab78220 the same antibody clone in a different buffer formulation.

Flow cytometric analysis of human PBMC+GM-CSF (100 ng/ml 3 days) + IL-4 (50 ng/ml 3 days) then GM-CSF (100 ng/ml 3 days) + IL-4 (50 ng/ml 3 days) (+) labelling CD1b with ab78220 at 1/1000 dilution (0.1 μg)/ Right compared with a Mouse monoclonal IgG / Left isotype control.

Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WM25

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab252262 is the carrier-free version of ab78220.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1b also known as CD1B is a member of the CD1 family of glycoproteins with a molecular mass around 37 to 40 kilodaltons. It is expressed on the surface of antigen-presenting cells such as dendritic cells and some monocytes. CD1b plays a mechanical role in binding lipid-based antigens. This binding facilitates the presentation of these antigens to T cells. Its presence on the cell surface is essential for this antigen presentation function.
Biological function summary

CD1b functions in the immune system as part of the antigen presentation complex. It specifically presents lipid antigens derived from microbial sources to T cells helping create an immune response. The protein associates with beta-2 microglobulin which stabilizes its structure on the cell surface. CD1b can effectively capture and present mycobacterial lipid antigens which are important for the immune response against certain bacterial infections.

Pathways

CD1b actively contributes to immunity by participating in the lipid antigen presentation pathway. This pathway involves the trafficking of endogenous and exogenous lipids aided by proteins like saposins. CD1b interacts with other CD1 family proteins such as CD1a and CD1c which are involved in similar pathways for presenting different types of lipid antigens to T cells therefore diversifying the immune response.

CD1b is implicated in tuberculosis as Mycobacterium tuberculosis interacts with this protein to modulate the host's immune defenses. Defective CD1b function can influence the body's response to this pathogen potentially leading to a chronic infection state. It also relates to autoimmune diseases such as multiple sclerosis where dysregulated lipid antigen presentation might influence disease progression. In these conditions interactions with proteins like HLA-DR can modify the immune landscape and disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1B
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Product promise

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