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AB246520

Anti-CD1c antibody [EPR23189-196]

5

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal CD1C antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD1c, T-cell surface glycoprotein CD1c, CD1C

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (AB246520)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (AB246520)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human Hodgkin's lymphoma. The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (AB246520)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (AB246520)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. (PMID : 15191950)The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Western blot - Anti-CD1c antibody [EPR23189-196] (AB246520)
  • WB

Lab

Western blot - Anti-CD1c antibody [EPR23189-196] (AB246520)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1385541).

Exposure times : Lane 1 : 15 seconds; Lane 2 : 37 seconds; Lanes 3 : 3 minutes.

All lanes:

Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520) at 1/1000 dilution

Lane 1:

Human thymus tissue lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 38 kDa

Observed band size: 43 kDa

false

Western blot - Anti-CD1c antibody [EPR23189-196] (AB246520)
  • WB

Lab

Western blot - Anti-CD1c antibody [EPR23189-196] (AB246520)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1385541).

Negative control : THP-1 (PMID : 24935257)

There is an unidentifiable protein at high molecular weight.

Exposure time : 26 seconds.

All lanes:

Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 2:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Lane 3:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 38 kDa

Observed band size: 43 kDa

false

  • Carrier free

    Anti-CD1c antibody [EPR23189-196] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23189-196

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1c also known as CD1c molecule or R7 is a transmembrane glycoprotein with a molecular weight of approximately 43 kDa. It belongs to the CD1 family of glycoproteins which share structural similarity to major histocompatibility complex (MHC) proteins. CD1c presents lipid and glycolipid antigens to T cells. It is expressed on professional antigen-presenting cells (APCs) like dendritic cells B cells and some monocytes. This expression allows CD1c to function in the immune response by presenting specialized antigens to T cells.
Biological function summary

The function of CD1c involves the presentation of lipid antigens which plays a role in immune recognition. CD1c is a part of a complex that includes β2-microglobulin which stabilizes its structure for antigen presentation. CD1c contributes to the immune surveillance by enabling the recognition of microbial lipid antigens as well as self-lipids by specific T cell subsets. This function supports the body's immune defense by identifying and reacting to potential pathogenic lipids.

Pathways

CD1c is involved in immune response pathways that center around antigen presentation. It interacts with other proteins like CD1a CD1b and CD1d expanding the range of antigens that T cells can recognize. CD1c participates in the lipid antigen presentation pathway influencing both innate and adaptive immunity. The presentation of lipid antigens by CD1c to T cells can trigger signaling pathways that affect both immune activation and resolution.

CD1c is implicated in autoimmune diseases and infections. Its role in antigen presentation makes it a potential factor in autoimmune conditions where immune recognition errors might occur. Additionally CD1c may relate to infectious diseases where mycobacteria or other pathogens present unique lipid antigens. Through its interaction with T cell receptors CD1c can impact how well immune responses are mounted against these challenges making it a notable target for therapeutic development and research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1C
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2152 PubMed40038288

2025

Single cell immunoprofile of synovial fluid in rheumatoid arthritis with TNF/JAK inhibitor treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xuyang Xia,Chenjia He,Zhinan Xue,Yuelan Wang,Yun Qin,Zhixiang Ren,Yupeng Huang,Han Luo,Hai-Ning Chen,Wei-Han Zhang,Li-Bin Huang,Yunying Shi,Yangjuan Bai,Bei Cai,Lanlan Wang,Feng Zhang,Maoxiang Qian,Wei Zhang,Yang Shu,Geng Yin,Heng Xu,Qibing Xie

Journal of immunology (Baltimore, Md. : 1950) 213:1023-1032 PubMed39132986

2024

Cell-surface Milieu Remodeling in Human Dendritic Cell Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Namrata D Udeshi,Charles Xu,Zuzhi Jiang,Shihong Max Gao,Qian Yin,Wei Luo,Steven A Carr,Mark M Davis,Jiefu Li

Journal for immunotherapy of cancer 12: PubMed38964786

2024

Hypoxia inhibits the iMo/cDC2/CD8+ TRMs immune axis in the tumor microenvironment of human esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanqiang Wu,Huan Yu,Fuxiang Liang,Xiancong Huang,Bin Jiang,Zhiling Lou,Yafei Liu,Zixiang Wu,Qi Wang,Hong Shen,Ming Chen,Pin Wu,Ming Wu

The Journal of experimental medicine 218: PubMed34279540

2021

Single-cell analysis of human skin identifies CD14+ type 3 dendritic cells co-producing IL1B and IL23A in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Nakamizo,Charles-Antoine Dutertre,Ahad Khalilnezhad,Xiao Meng Zhang,Shawn Lim,Josephine Lum,Geraldine Koh,Charlene Foong,Pearly Jean Ai Yong,Kahbing Jasmine Tan,Reiko Sato,Kaori Tomari,Laurent Yvan-Charvet,Helen He,Emma Guttman-Yassky,Benoit Malleret,Rintaro Shibuya,Masashi Iwata,Baptiste Janela,Tsuyoshi Goto,Tan Siyun Lucinda,Mark B Y Tang,Colin Theng,Valerie Julia,Feriel Hacini-Rachinel,Kenji Kabashima,Florent Ginhoux
View all publications
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Product promise

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