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Rabbit Recombinant Monoclonal CD1C antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (AB246520), expandable thumbnail
  • Western blot - Anti-CD1c antibody [EPR23189-196] (AB246520), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (AB246520), expandable thumbnail
  • Western blot - Anti-CD1c antibody [EPR23189-196] (AB246520), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species

Rat

Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Alternative names

CD1c, T-cell surface glycoprotein CD1c, CD1C

Recommended products

Rabbit Recombinant Monoclonal CD1C antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23189-196

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD1c also known as CD1c molecule or R7 is a transmembrane glycoprotein with a molecular weight of approximately 43 kDa. It belongs to the CD1 family of glycoproteins which share structural similarity to major histocompatibility complex (MHC) proteins. CD1c presents lipid and glycolipid antigens to T cells. It is expressed on professional antigen-presenting cells (APCs) like dendritic cells B cells and some monocytes. This expression allows CD1c to function in the immune response by presenting specialized antigens to T cells.

Biological function summary

The function of CD1c involves the presentation of lipid antigens which plays a role in immune recognition. CD1c is a part of a complex that includes β2-microglobulin which stabilizes its structure for antigen presentation. CD1c contributes to the immune surveillance by enabling the recognition of microbial lipid antigens as well as self-lipids by specific T cell subsets. This function supports the body's immune defense by identifying and reacting to potential pathogenic lipids.

Pathways

CD1c is involved in immune response pathways that center around antigen presentation. It interacts with other proteins like CD1a CD1b and CD1d expanding the range of antigens that T cells can recognize. CD1c participates in the lipid antigen presentation pathway influencing both innate and adaptive immunity. The presentation of lipid antigens by CD1c to T cells can trigger signaling pathways that affect both immune activation and resolution.

Associated diseases and disorders

CD1c is implicated in autoimmune diseases and infections. Its role in antigen presentation makes it a potential factor in autoimmune conditions where immune recognition errors might occur. Additionally CD1c may relate to infectious diseases where mycobacteria or other pathogens present unique lipid antigens. Through its interaction with T cell receptors CD1c can impact how well immune responses are mounted against these challenges making it a notable target for therapeutic development and research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520)

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human Hodgkin's lymphoma. The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520), expandable thumbnail

    Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1385541).

    Exposure times: Lane 1: 15 seconds; Lane 2: 37 seconds; Lanes 3: 3 minutes.

    All lanes: Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520) at 1/1000 dilution

    Lane 1: Human thymus tissue lysate at 20 µg

    Lane 2: Human tonsil tissue lysate at 20 µg

    Lane 3: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 38 kDa

    Observed band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] (ab246520)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human spleen. (PMID: 15191950)The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520), expandable thumbnail

    Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 1385541).

    Negative control: THP-1 (PMID: 24935257)

    There is an unidentifiable protein at high molecular weight.

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-CD1c antibody [EPR23189-196] (ab246520) at 1/1000 dilution

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 2: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

    Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa

    Observed band size: 43 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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