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AB229903

Anti-CD1d antibody [EPR22526-72]

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(1 Publication)

Rabbit Recombinant Monoclonal CD1D antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD1d, Antigen-presenting glycoprotein CD1d, R3G1, CD1D

2 Images
Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] (AB229903)
  • IP

Unknown

Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] (AB229903)

CD1d was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole lysate with ab229903 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229903 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : Jurkat whole cell lysate 10 μg (Input).

Lane 2 : ab229903 IP in Jurkat whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229903 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CD1d antibody [EPR22526-72] (ab229903)

Predicted band size: 38 kDa

Observed band size: 48 kDa

false

Western blot - Anti-CD1d antibody [EPR22526-72] (AB229903)
  • WB

Unknown

Western blot - Anti-CD1d antibody [EPR22526-72] (AB229903)

Exposure time : Lane 1 : 3 minutes; Lanes 2-6 : 48 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 10809957).

Negative control : SH-SY5Y (PMID : 26749374), K562 (PMID : 21037579).

All lanes:

Western blot - Anti-CD1d antibody [EPR22526-72] (ab229903) at 1/1000 dilution

Lane 1:

Human lymph node lysate at 20 µg

Lane 2:

Human thymus lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 4:

MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

Lane 5:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg

Lane 6:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 38 kDa

Observed band size: 48 kDa

false

  • Carrier free

    Anti-CD1d antibody [EPR22526-72] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22526-72

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1d is a non-classical major histocompatibility complex (MHC) class I-like molecule also known as the CD1d molecule. This protein has a mass of approximately 37 kDa and plays an important role in the presentation of lipid antigens to T cells. CD1d is mainly expressed on the surface of antigen-presenting cells including dendritic cells B cells and epithelial cells in the intestine. The expression pattern highlights its relevance in immune responses at mucosal surfaces.
Biological function summary

CD1d functions by presenting lipid and glycolipid antigens to a specialized subset of T cells called invariant Natural Killer T (iNKT) cells. CD1d forms a complex with these antigens in the endosomal compartment before migrating to the cell surface. This interaction is critical for activating iNKT cells which then produce cytokines that influence both innate and adaptive immune responses. It is distinct in its role compared to classical MHC molecules in antigen presentation.

Pathways

CD1d's role in antigen presentation influences numerous immune signaling pathways. Its interaction with iNKT cells is part of the broader immune regulatory network impacting the Th1/Th2 cytokine balance and contributing to immune surveillance. CD1d works alongside proteins involved in these pathways such as CD1b and CD1c which also present lipids to T cells highlighting their involvement in maintaining immune system homeostasis.

CD1d has been linked to autoimmune diseases and cancers. Within autoimmune disorders the interaction between CD1d and iNKT cells affects disease pathogenesis possibly through cytokine dysregulation. In some cancers CD1d expression changes can disrupt normal immune function allowing tumors to progress. Through these diseases CD1d interacts with proteins such as Nor32 and Nor3 impacting how immune cells recognize and respond to diseased or cancerous cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
See full target information CD1D
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102217 PubMed40834855

2025

Genomic landscape and immunological profile of glioblastoma in East Asian patients.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Zhong,Bo Wu,Frank Dubois,Davy Deng,Johann S Bergholz,Wanming Hu,Hao Duan,Yujie Shao,Jingyi Liu,Luwei Liu,Sitong Chen,Yongfei Hu,Zheng Zhao,Maojin Yao,Ke Sai,Jian Wang,Yinsheng Chen,Chao Ke,Xiangheng Zhang,Qunying Yang,Chengcheng Guo,Ji Zhang,Fuhua Lin,Zhenghe Chen,Deipei Li,Zhenqiang He,Chang Li,Xiaobing Jiang,Jie Lu,Wenzhuo Yang,Tao Jiang,Zhongping Chen,Rameen Beroukhim,Jean J Zhao,Yonggao Mou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com