Rabbit Recombinant Monoclonal CD1D antibody. Carrier free. Suitable for IP, Flow Cyt, WB and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357), expandable thumbnail](https://content.abcam.com/products/images/cd1d-antibody-epr22526-9-bsa-and-azide-free-ab256357--flow-cytometry-img67775.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)")
![Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357), expandable thumbnail](https://content.abcam.com/products/images/cd1d-antibody-epr22526-9-bsa-and-azide-free-ab256357--flow-cytometry-img17734.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)")
![Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357), expandable thumbnail](https://content.abcam.com/products/images/cd1d-antibody-epr22526-9-bsa-and-azide-free-ab256357--immunoprecipitation-img138817.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
Alternative names
CD1d, Antigen-presenting glycoprotein CD1d, R3G1, CD1D
Recommended products
Rabbit Recombinant Monoclonal CD1D antibody. Carrier free. Suitable for IP, Flow Cyt, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22526-9
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256357 is the carrier-free version of Anti-CD1d antibody [EPR22526-9] ab256344.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD1d is a non-classical major histocompatibility complex (MHC) class I-like molecule also known as the CD1d molecule. This protein has a mass of approximately 37 kDa and plays an important role in the presentation of lipid antigens to T cells. CD1d is mainly expressed on the surface of antigen-presenting cells including dendritic cells B cells and epithelial cells in the intestine. The expression pattern highlights its relevance in immune responses at mucosal surfaces.
- Biological function summary
CD1d functions by presenting lipid and glycolipid antigens to a specialized subset of T cells called invariant Natural Killer T (iNKT) cells. CD1d forms a complex with these antigens in the endosomal compartment before migrating to the cell surface. This interaction is critical for activating iNKT cells which then produce cytokines that influence both innate and adaptive immune responses. It is distinct in its role compared to classical MHC molecules in antigen presentation.
- Pathways
CD1d's role in antigen presentation influences numerous immune signaling pathways. Its interaction with iNKT cells is part of the broader immune regulatory network impacting the Th1/Th2 cytokine balance and contributing to immune surveillance. CD1d works alongside proteins involved in these pathways such as CD1b and CD1c which also present lipids to T cells highlighting their involvement in maintaining immune system homeostasis.
- Associated diseases and disorders
CD1d has been linked to autoimmune diseases and cancers. Within autoimmune disorders the interaction between CD1d and iNKT cells affects disease pathogenesis possibly through cytokine dysregulation. In some cancers CD1d expression changes can disrupt normal immune function allowing tumors to progress. Through these diseases CD1d interacts with proteins such as Nor32 and Nor3 impacting how immune cells recognize and respond to diseased or cancerous cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357), expandable thumbnail](https://content.abcam.com/products/images/cd1d-antibody-epr22526-9-bsa-and-azide-free-ab256357--flow-cytometry-img67775.jpg "Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357)")
Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357)
Flow cytometric analysis of K562 (human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling CD1d with Anti-CD1d antibody [EPR22526-9] ab256344 at 1/600 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Negative control: K562 (PMID: 21037579).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD1d antibody [EPR22526-9] ab256344).
![Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357), expandable thumbnail](https://content.abcam.com/products/images/cd1d-antibody-epr22526-9-bsa-and-azide-free-ab256357--flow-cytometry-img17734.jpg "Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357)")
Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD1d with Anti-CD1d antibody [EPR22526-9] ab256344 at 1/600 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or Anti-CD1d antibody [EPR22526-9] ab256344 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD1d antibody [EPR22526-9] ab256344).
![Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357), expandable thumbnail](https://content.abcam.com/products/images/cd1d-antibody-epr22526-9-bsa-and-azide-free-ab256357--immunoprecipitation-img138817.jpg "Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357)")
Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (ab256357)
CD1d was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with Anti-CD1d antibody [EPR22526-9] ab256344 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD1d antibody [EPR22526-9] ab256344 at 1/1000 dilution (0.6 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate (10μg)
Lane 2: Anti-CD1d antibody [EPR22526-9] ab256344 IP in Jurkat whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD1d antibody [EPR22526-9] ab256344 in Jurkat whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD1d antibody [EPR22526-9] ab256344).
All lanes: Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] (Anti-CD1d antibody [EPR22526-9] ab256344)
Predicted band size: 38 kDa
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