AB256357

Anti-CD1d antibody [EPR22526-9] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD1D antibody. Carrier free. Suitable for IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD1d, Antigen-presenting glycoprotein CD1d, R3G1, CD1D

3 Images

Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Flow Cyt

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Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia lymphoblast, Left) / Jurkat (human T cell leukemia T lymphocyte, Right) cells labeling CD1d with ab256344 at 1/600 dilution (0.1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Negative control : K562 (PMID : 21037579).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256344).

Flow Cyt

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Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD1d with ab256344 at 1/600 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150097) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab256344 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor^® 647. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256344).

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Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

CD1d was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate with ab256344 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256344 at 1/1000 dilution (0.6 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : Jurkat (human T cell leukemia T lymphocyte) whole cell lysate (10μg)
Lane 2 : ab256344 IP in Jurkat whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256344 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256344).

All lanes:

Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] (<a href='/en-us/products/primary-antibodies/cd1d-antibody-epr22526-9-ab256344'>ab256344</a>)

Predicted band size: 38 kDa

false

Unconjugated

Anti-CD1d antibody [EPR22526-9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22526-9

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab256357 is the carrier-free version of ab256344.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD1d is a non-classical major histocompatibility complex (MHC) class I-like molecule also known as the CD1d molecule. This protein has a mass of approximately 37 kDa and plays an important role in the presentation of lipid antigens to T cells. CD1d is mainly expressed on the surface of antigen-presenting cells including dendritic cells B cells and epithelial cells in the intestine. The expression pattern highlights its relevance in immune responses at mucosal surfaces.

Biological function summary

CD1d functions by presenting lipid and glycolipid antigens to a specialized subset of T cells called invariant Natural Killer T (iNKT) cells. CD1d forms a complex with these antigens in the endosomal compartment before migrating to the cell surface. This interaction is critical for activating iNKT cells which then produce cytokines that influence both innate and adaptive immune responses. It is distinct in its role compared to classical MHC molecules in antigen presentation.

Pathways

CD1d's role in antigen presentation influences numerous immune signaling pathways. Its interaction with iNKT cells is part of the broader immune regulatory network impacting the Th1/Th2 cytokine balance and contributing to immune surveillance. CD1d works alongside proteins involved in these pathways such as CD1b and CD1c which also present lipids to T cells highlighting their involvement in maintaining immune system homeostasis.

CD1d has been linked to autoimmune diseases and cancers. Within autoimmune disorders the interaction between CD1d and iNKT cells affects disease pathogenesis possibly through cytokine dysregulation. In some cancers CD1d expression changes can disrupt normal immune function allowing tumors to progress. Through these diseases CD1d interacts with proteins such as Nor32 and Nor3 impacting how immune cells recognize and respond to diseased or cancerous cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

See full target information CD1D

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2664:185-199 PubMed37423991

2023

Quantitative Imaging of Podocyte Foot Processes in the Kidney Using Confocal and STED Microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

David Unnersjö-Jess

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD1d antibody [EPR22526-9] - BSA and Azide free

Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Flow Cytometry - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Immunoprecipitation - Anti-CD1d antibody [EPR22526-9] - BSA and Azide free (AB256357)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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