AB212988

Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free

Name: Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (ab212988) | Abcam
Brand: Abcam Limited
SKU: AB212988

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Mouse Monoclonal CD2 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD2 protein.

View Alternative Names

CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

3 Images

Flow Cytometry - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Flow Cytometric Analysis of MOLT4 cells using ab193344 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193344).

Immunocytochemistry/ Immunofluorescence - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

Immunofluorescence staining of MOLT-4 cells using ab193344 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193344).

Supplier Data

Western blot - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (AB212988)

All lanes:

Western blot - Anti-CD2 antibody [1E7E8.G4] - BSA and Azide free (ab212988) at 2 µg/mL

All lanes:

Jurkat cell lysate

Predicted band size: 39 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1E7E8.G4

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

Cell preparation containing CD2 protein. The exact immunogen used to generate this antibody is proprietary information.

P06729

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

ab212988 is a carrier free version of ab193344.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.

Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

See full target information CD2

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Product promise

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