AB219411

Anti-CD2 antibody [EPR21825]

20ul selling size
RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal CD2 antibody. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

CD2, Ly-37, Cd2, T-cell surface antigen CD2, LFA-2, LFA-3 receptor, Lymphocyte antigen 37, T-cell surface antigen T11/Leu-5

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD2 with ab219411 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse thymus (PMID : 2901356) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Flow Cytometry - Anti-CD2 antibody [EPR21825] (AB219411)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD2 antibody [EPR21825] (AB219411)

Flow cytometric analysis of mouse primary splenocytes labeling CD2 with ab219411 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were surface stained with anti-mouse CD19-Alexa Fluor^®647 and rabbit IgG (left) / ab219411 (right). Gated on total viable cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD2 with ab219411 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse spleen (PMID : 2901356) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Immunoprecipitation - Anti-CD2 antibody [EPR21825] (AB219411)

CD2 was immunoprecipitated from 0.35 mg of mouse thymus lysate with ab219411 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219411 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : Mouse thymus lysate 10 μg (Input).

Lane 2 : ab219411 IP in mouse thymus lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219411 in mouse thymus lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-CD2 antibody [EPR21825] (ab219411)

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-CD2 antibody [EPR21825] (AB219411)

Blocking/Dilution buffer : 5% NFDM/TBST.

Molecular mass is consistent with the literature (PMID 10510361).

All lanes:

Western blot - Anti-CD2 antibody [EPR21825] (ab219411) at 1/1000 dilution

All lanes:

Mouse thymus lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 45-58 kDa

true

Exposure time: 3min

Carrier free

Anti-CD2 antibody [EPR21825] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21825

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

Flow Cyt, WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.

Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

See full target information Cd2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 13: PubMed40953922

2025

Pancreatic cancer cells escape T/NK cell immune surveillance through the expressional separation of CD58.

Applications

Unspecified application

Species

Unspecified reactive species

Yalu Zhang,Huaijin Zheng,Ronghua Zhang,Jiayi Li,Sen Yang,Yuze Hua,Jorg Kleeff,Quan Liao,Qiaofei Liu

Molecular and cellular biochemistry 480:6225-6238 PubMed40859095

2025

NETs accelerate aortic valve calcification by promoting M1 macrophage polarization through the TLR9 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Luyao Liu,Zhenzhen Lan,Xincan Liu,Yun Chen,Zhihao Chen,Li Cheng,Ting Hu

Frontiers in immunology 16:1482070 PubMed40129982

2025

Generation and characterization of a tamoxifen-inducible lineage tracing tool knock-in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Guo,Mengyan Zhu,Zhilan Yu,Qing Li,Yanjuan Chen,Lei Ci,Ruilin Sun,Ruling Shen

Frontiers in medicine 9:827695 PubMed35602471

2022

Identification and Validation of Immune Molecular Subtypes and Immune Landscape Based on Colon Cancer Cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Qi,Qian Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD2 antibody [EPR21825]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

Flow Cytometry - Anti-CD2 antibody [EPR21825] (AB219411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [EPR21825] (AB219411)

Immunoprecipitation - Anti-CD2 antibody [EPR21825] (AB219411)

Western blot - Anti-CD2 antibody [EPR21825] (AB219411)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise