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Rabbit Recombinant Monoclonal CD2 antibody. Suitable for IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (AB227698), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (AB227698), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (AB227698), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (AB227698), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (AB227698), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Incubate with primary antibody for 10 minutes at room temperature.

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Alternative names

CD2, SRBC, T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5

Recommended products

Rabbit Recombinant Monoclonal CD2 antibody. Suitable for IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SP304
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD2 also known as T11 or LFA-2 acts as a cell surface glycoprotein expressed mainly on T-cells natural killer cells and thymocytes. This protein has a molecular mass of approximately 50-55 kDa. CD2 plays a critical role in facilitating cell-cell adhesion and signal transduction. It serves as a receptor for the CD58 ligand on antigen-presenting cells supporting the interaction between T-cells and these cells. By enhancing adhesion CD2 contributes to the formation of the immunological synapse necessary for effective immune response.

Biological function summary

The functioning of CD2 helps in the modulation of immune responses and T-cell activation. CD2 participates in signal transduction leading to cytokine production and proliferation. It is associated with the immunological synapse complex working closely with other surface molecules like Lck and CD3 to promote T-cell responses. This collaboration is vital for the development and function of immune cells driving adaptive immunity.

Pathways

CD2 engages in pathways integral to T-cell activation and signal transduction. A well-known pathway involving CD2 is the TCR (T-cell Receptor) signaling pathway where it partners with CD3 to initiate T-cell activation. Additionally CD2 influences the LAT (Linker for Activation of T-cells) pathway contributing to downstream signaling cascades that involve various signaling proteins such as Lck and ZAP-70 ensuring proper T-cell function.

Associated diseases and disorders

CD2 shows connections to autoimmune diseases and cancers. For instance its involvement in abnormal T-cell activation links CD2 to autoimmune conditions like rheumatoid arthritis. Also various leukemias demonstrate altered CD2 expression suggesting a role in cancer pathogenesis. Through these disease states CD2's interaction with the CD58 protein becomes critical as this interaction affects the activity of T-cells in the disease milieu influencing disease progression and treatment outcomes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human thymus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human uterus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD2 antibody [SP304] (ab227698)

    Immunohistochemistry analysis of paraffin-embedded human tonsil tissue sections labelling CD2 with ab227698 at 1/100 dilution. The section was incubated with ab227698 for 10 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
    Positive staining on the human tonsil tissue, performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com